Navegando por Autor "Tim, Carla Roberta"

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Abordagens inovadoras no reparo de feridas com óleo ozonizado e terapia por fotobiomodulação
(Universidade Brasil, 2024) Pimentel, Priscila Cristina Oliveira Zignani; Tim, Carla Roberta
Hard-to-heal wounds present significant challenges in their therapeutic context due to the presence of pathogenic microorganisms and associated infections, thus resulting in delays for the tissue-healing process. The present study aimed to carry out a clinical trial to identify the microbial agent present in hard-to-heal wounds on the lower limbs, assess the antimicrobial effects of ozonated oil and examine the benefits of photobiomodulation combined with ozonated oil in the treatment of those wounds. Then we examined the antimicrobial kinetics of ozonated oil on microorganisms found in wounds by an in vitro study. For this study, some commercial ozonated sunflower oil was used as well as the LED photobiomodulation therapy, 660 nm, 30 seconds, punctual 2 cm away on the wound bed and on the margins in contact with the skin, 120 mW, 3.6 J per point. The procedure was performed three times a week, for a period of 12 weeks. The clinical results evidenced a significant healing of injuries treated with ozonated oil and photobiomodulation, evidenced by a reduction in traces of infection, presence of granulation tissue, wound area reduction and, in some cases, full wound closure. Among the microorganisms identified are Klebsiella sp., Staphylococcus epidermidis and Escherichia coli, all proved resistant to the antibiotics tested. The results obtained in vitro revealed that the ozonated oil was effective in reducing and eliminating colony-forming units in all microorganisms tested when subjected to concentrations deemed low, ranging from 1.6% to 12.5%, demonstrating antibacterial activity. In conclusion, ozonized oil, associated with photobiomodulation therapy, demonstrated efficacy in the treatment of wounds that are difficult to repair. However, new clinical trials are necessary to establish more improved treatment protocols.
Análises epidemiológica e espacial da Covid-19 no Estado do Piauí
(Universidade Brasil, 2021) Macedo, Glauber Bezerra; Tim, Carla Roberta; Garcia, Lívia Assis
The emergence of a new coronavirus in humans, first diagnosed in 2019, has many deaths and serious survival and psychosocial consequences. Through statistical tools and techniques associated with geographic technologies, this ecological, analytical and exploratory study is being developed, whose general objective is to carry out epidemiological and spatial analyzes of the distribution of confirmed cases and deaths from Coronavirus 2019 disease (Coronavirus Disease 2019 - COVID-19), in the year 2020, in the state of Piauí, Brazil. The variables listed are being assigned via the IBGE and SESAPI database. All confirmed cases and deaths with COVID-19 infection that were reported in 2020 in that state were included in the study. In the epidemiological analyzes descriptive statistics were carried out and in the spatial analyses, maps are being constructed using geoprocessing techniques, through the statistical analysis of Moran Global and Local. Preliminary results showed that, in 2020, 143,179 confirmed cases and 2,840 deaths caused by COVID-19 were reported in Piauí. A higher percentage of cases was observed in relation to females, young adults, and deaths in elderly, males, with chronic diseases. The capital Piauiense led the number of cases and deaths from the disease, probably due to the high population density. However, when considering the incidence and mortality coefficients of COVID-19, the highest rates in the state were considered in the municipalities of União and Água Branca, respectively. The spatial analysis of the cases showed clusters of high-high pattern of cases of the disease in the metropolitan region of Teresina, the region between Rios and the region of Tabuleiros do Alto do Parnaíba. A high-high cluster for mortality was verified in fillings in the region of Entre Rios and Vale do Sambito. The analyzes performed provided the visualization of spatial clusters and an identification of vulnerable areas, resulting in information that was not visualized working only with tabular data.
Análises epidemiológica e espacial da Covid-19 no Estado do Piauí
(Universidade Brasil, 2021) Macedo, Glauber Bezerra; Garcia, Lívia Assis; Tim, Carla Roberta
The emergence of a new coronavirus in humans, first diagnosed in 2019, has many deaths and serious survival and psychosocial consequences. Through statistical tools and techniques associated with geographic technologies, this ecological, analytical and exploratory study is being developed, whose general objective is to carry out epidemiological and spatial analyzes of the distribution of confirmed cases and deaths from Coronavirus 2019 disease (Coronavirus Disease 2019 - COVID-19), in the year 2020, in the state of Piauí, Brazil. The variables listed are being assigned via the IBGE and SESAPI database. All confirmed cases and deaths with COVID-19 infection that were reported in 2020 in that state were included in the study. In the epidemiological analyzes descriptive statistics were carried out and in the spatial analyses, maps are being constructed using geoprocessing techniques, through the statistical analysis of Moran Global and Local. Preliminary results showed that, in 2020, 143,179 confirmed cases and 2,840 deaths caused by COVID-19 were reported in Piauí. A higher percentage of cases was observed in relation to females, young adults, and deaths in elderly, males, with chronic diseases. The capital Piauiense led the number of cases and deaths from the disease, probably due to the high population density. However, when considering the incidence and mortality coefficients of COVID-19, the highest rates in the state were considered in the municipalities of União and Água Branca, respectively. The spatial analysis of the cases showed clusters of high-high pattern of cases of the disease in the metropolitan region of Teresina, the region between Rios and the region of Tabuleiros do Alto do Parnaíba. A high-high cluster for mortality was verified in fillings in the region of Entre Rios and Vale do Sambito. The analyzes performed provided the visualization of spatial clusters and an identification of vulnerable areas, resulting in information that was not visualized working only with tabular data.
Avaliação da atividade antimicrobiana da terapia fotodinâmica no tratamento da microbiota bucal
(Universidade Brasil, 2022) Moreti, Lucieni Cristina Trovati; Tim, Carla Roberta; Assis, Lívia
Photodynamic Therapy (PDT) is based on the interaction of light with a photosensitizer capable of promoting microbial destruction. In the present work, the antimicrobial activity of aPDT was evaluated in vitro using two photosensitizers (phenothiazine and annatto) and different times of treatment of oral biofilm, as well as microorganisms were identified and bacterial resistance and sensitivity to certain antibiotics were evaluated. For this, antimicrobial samples of oral biofilm (tongue) were collected from patients without and with pneumonia admitted to the ICU, who were intubated for a period of at least 24 and at most 48 hours, using swabs soaked in saline solution (NaCl 0.5 %) and later cultivated in agarized media. Samples from patients without pneumonia were randomized into: non-pneumonia control group (NP - C): microorganisms without intervention; non-pneumonia chlorhexidine group (NP - CLX): used 0.12% chlorhexidine; non-pneumonia group red PDT (NP - PDTV): used aPDT using 630 nm LED and phenothiazine photosensitizer; non-pneumonia blue PDT group (NP - PDTA): used the use of aPDT using 450 nm LED and annatto photosensitizer, both using a single application. And control pneumonia group (P - C): microorganisms without intervention; group with chlorhexidine pneumonia (P ¬ CLX): used 0.12% chlorhexidine; group with red PDT pneumonia (P - PDTV): used the use of PDT using 630 nm LED and phenothiazine photosensitizer; group with blue PDT pneumonia (P - PDTA): used the use of PDT using 450 nm LED and annatto photosensitizer. They were irradiated with the following parameters: red LED (λ630 ± 10ηm) or blue LED (λ450 ± 10ηm), both at 100 mW power and treated for 150, 300 or 600 seconds. After 24 hours, colony forming units (CFU) and antibiogram were counted. The results showed that the NP – CLX and P – CLX groups promoted total CFU reduction. The PDT groups in oral biofilm without and with pneumonia showed that the CFU reduction was dependent on the treatment time, being better at 600 seconds of treatment. Through the genetic code, Gram-negative microorganisms such as Pseudomonas aeruginosa and Gram-positive microorganisms such as Bhargavaea sp., Desemzia incerta, Kocuria rosea, Micrococcus sp. ,Micrococcus luteus, Agrococcus carbonis, Microbacterium paraoxydans, Corynebacterium sp., Dietzia aurantiaca, Enterococcus faecalis and Staphylococcus saprophyticus.The antibiogram performed evaluated the multiple antimicrobial resistance index (IRMA) characterizing multiresistance to all. Conclusion: Despite being composed of multidrug-resistant microorganisms, aPDT showed potential for the treatment of oral biofilm in patients with and without pneumonia.
Avaliação de diferentes concentrações de água ozonizada na desinfecção de canais radiculares contaminados: estudo in vitro
(Universidade Brasil, 2022) Fernandes, Karina Gonzalez Camara; Garcia, Lívia Assis; Tim, Carla Roberta
In the clinical practice of dentistry, it is evident that despite technological advances, microbial agents are still the main responsible for endodontic failure. Mechanical chemical preparation, a technique used in endodontics, aims to remove necrotic tissue, microbial agents and their by-products from the root canal system. However, preparation is often not sufficient for satisfactory cleaning due to the anatomical complexity of the root canal system, which may lead to the permanence of microorganisms that may remain viable and active, interfering with the recovery process of periradicular tissues. In this way, it is necessary to search for ideal irrigating solutions that can provide better results in endodontic treatment. The aim of the present study was to evaluate and compare, in vitro, the effectiveness of different concentrations of ozonated water in the disinfection of root canals inoculated with Enterococcus Faecalis (E. Faecalis). We used 75 extracted human premolars, with a single canal, approved by the Research Ethics Committee (CEP), approval number 26629619.9.0000.5494. The teeth were sectioned and contaminated with E. Faecalis and incubated for 21 days at 37°C. Initial collections were performed with absorbent paper cones to confirm contamination. subsequently, PQM mechanical chemical preparation was performed, using mechanical instrumentation with a WaveOne® Primary file and the samples were divided into 5 groups according to the irrigating solutions (n = 15): Saline Solution Group (SS): irrigating solution was saline; 2.5% sodium hypochlorite group (NaOCl): 2.5% sodium hypochlorite irrigating solution; Ozone Group 10 (O10): the irrigating solution of ozonized water at a concentration of 10 µg/mL; Ozone Group 20 (O20): irrigating solution of ozonized water at a concentrationof 20 µg/mL; Ozone Group 30 (O30): irrigating solution of ozonized water at a concentration of 30 µg/mL. After mechanical instrumentation and irrigation, a second collection was performed with a paper cone, so the variables analyzed were the collections before and after the PQM. The main results show that the ozonated water used as an irrigating solution in the mechanical chemical preparation was effective in reducing the number of CFU Colony Forming Units at all concentrations (O10, 17,48%O20, 18,87% and O30, 32,87%) and the highest percentage of reduction was observed at the concentration of 30 μg/mL. However, it was not possible to observe a higher reduction of CFU in the groups treated with ozonated water when compared to the irrigant NaOCl. In conclusion, the ozonated water used as an irrigating solution, with emphasis on the concentration of 30 μg/mL, promoted antimicrobial action against E. Faecalis, one of the main microorganisms responsible for critical complications in endodontics, and can be proposed as an adjuvant in endodontic treatments.
Avaliação dos efeitos fototérmico do laser de diodo λ1470 nm na pele em modelo experimental de ratos.
(Universidade Brasil, 2024) Godoi, Silvana Aparecida Girão de; Assis, Lívia; Tim, Carla Roberta
The subdermal high-power diode laser is a therapy that has been frequently used as a treatment for skin aging. However, there is a need for studies to verify morphological and molecular changes in the skin triggered by this light technology. Thus, the objective of the present study was to evaluate the photothermal effects of diode laser wavelength (λ) 1470 nm on the skin of rats. 20 male Wistar rats were used, randomized into 2 groups: placebo laser (LP): treatment with subdermal laser turned off; active laser (LA): subdermal laser treatment was used. For therapy, high-power diode laser equipment was used (λ = 1470 nm; P = 2 W; beam area = 240 μm; beam divergence in rad: 0.227; continuous mode), applied in 5 subdermal vectors in the region of the animal's back in an area of 24 cm2 depositing an average of energy of 85 J/cm2, in a single session and the tissue was evaluated 15 days after the intervention. To evaluate the effects of the treatment, histological, morphometric analyzes and immunoexpression of transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) were performed. The results demonstrated that active laser animals showed a better arrangement of collagen fiber bands and an increase in the thickness of the dermis and the number of vessels. Furthermore, animals treated with active laser showed an increased immunoexpression of TGF-β and VEGF compared to placebo. Thus, we can conclude that the subdermal high-power diode laser proves to be a promising option in aesthetic dermatology, as it was capable of increasing vascularization and the expression of factors that enhance skin rejuvenation.
Avaliação e comparação de diferentes comprimentos de onda (660 e 808 nm) da terapia por fotobiomodulação a laser na atrofia muscular em modelo de imobilização em ratos
(Universidade Brasil, 2021) Gonçalves, Silma Rodrigues; Garcia, Lívia Assis; Tim, Carla Roberta
Atrophy of skeletal striated muscle tissue is a complex process caused by an imbalance between the degradation and synthesis of myofibrillar proteins, leading to a reduction in muscle strength and the quality of life individuals. Considerable efforts have been devoted to establish new treatments for an effective and safe clinical for treatment of muscle atrophy. Among the available therapeutic resources, photobiomodulation therapy (PBMT) has great potential, as it is often used as a promising therapeutic strategy for the rehabilitation of skeletal striated muscle tissue, however, there is no consensus on the best parameters in the treatment of muscle atrophy. Thus, the aim of the present study was to evaluate the in vivo response of PBMT to laser at red and infrared wavelengths in muscle atrophy in an immobilization model in rats. 32 male Wistar rats were used, divided into 4 groups (n = 8): control group (C); Immobilized group (ImC); Immobilized group submitted to PBMT laser at red wavelength (ImR); Immobilized group submitted to laser PBMT at the infrared wavelength (ImIR). The left hind limb was immobilized in extension for 5 days. For laser PBMT, equipment was used (λ = 660 and 808 nm; P = 30 mW; t = 47 s; D = 50 J/cm²) applied at two points in the gastrocnemius muscle (cranial and caudal). Treatments started immediately after removal of the immobilization, with 24-hour intervals, totaling 9 sessions. To evaluate and compare the treatments, histological analysis were performed, as well as the profile area, cell density and ATPase histochemistry. Histological results showed fibers of varied shape, infiltration of inflammatory cells, and thickening of the connective tissue, reduced profile and increased density of muscle fibers in all immobilized animals. Furthermore, it was possible to verify a modulation of the inflammatory process and a lesser thickening of the intramuscular connective tissue in both ImR and ImIR groups, however, these results were more pronounced in the ImIR group, associated with morphological findings of regenerating fibers and an increase in the number of fibers oxidative (type I fibers). Thus, we can conclude that laser PBMT at both wavelengths were effective in changing the morphology of the gastrocnemius muscle subjected to atrophy in an experimental immobilization model, reducing the inflammatory infiltrate and the formation of intramuscular connective tissue. However, RI promoted a more evident positive effect by increasing regenerating muscle fibers and the number of oxidative fibers, which may be a promising resource in the clinical treatment of muscle atrophy.
Avaliação e comparação de diferentes comprimentos de onda (660 e 808 NM) da terapia por fotobiomodulação a laser na atrofia muscular em modelo de imobilização em ratos
(Universidade Brasil, 2021) Gonçalves, Silma Rodrigues; Tim, Carla Roberta; Garcia, Lívia Assis
Atrophy of skeletal striated muscle tissue is a complex process caused by an imbalance between the degradation and synthesis of myofibrillar proteins, leading to a reduction in muscle strength and the quality of life individuals. Considerable efforts have been devoted to establish new treatments for an effective and safe clinical for treatment of muscle atrophy. Among the available therapeutic resources, photobiomodulation therapy (PBMT) has great potential, as it is often used as a promising therapeutic strategy for the rehabilitation of skeletal striated muscle tissue, however, there is no consensus on the best parameters in the treatment of muscle atrophy. Thus, the aim of the present study was to evaluate the in vivo response of PBMT to laser at red and infrared wavelengths in muscle atrophy in an immobilization model in rats. 32 male Wistar rats were used, divided into 4 groups (n = 8): control group (C); Immobilized group (ImC); Immobilized group submitted to PBMT laser at red wavelength (ImR); Immobilized group submitted to laser PBMT at the infrared wavelength (ImIR). The left hind limb was immobilized in extension for 5 days. For laser PBMT, equipment was used (λ = 660 and 808 nm; P = 30 mW; t = 47 s; D = 50 J/cm²) applied at two points in the gastrocnemius muscle (cranial and caudal). Treatments started immediately after removal of the immobilization, with 24-hour intervals, totaling 9 sessions. To evaluate and compare the treatments, histological analysis were performed, as well as the profile area, cell density and ATPase histochemistry. Histological results showed fibers of varied shape, infiltration of inflammatory cells, and thickening of the connective tissue, reduced profile and increased density of muscle fibers in all immobilized animals. Furthermore, it was possible to verify a modulation of the inflammatory process and a lesser thickening of the intramuscular connective tissue in both ImR and ImIR groups, however, these results were more pronounced in the ImIR group, associated with morphological findings of regenerating fibers and an increase in the number of fibers oxidative (type I fibers). Thus, we can conclude that laser PBMT at both wavelengths were effective in changing the morphology of the gastrocnemius muscle subjected to atrophy in an experimental immobilization model, reducing the inflammatory infiltrate and the formation of intramuscular connective tissue. However, RI promoted a more evident positive effect by increasing regenerating muscle fibers and the number of oxidative fibers, which may be a promising resource in the clinical treatment of muscle atrophy.
Avaliação in vitro do potencial antimicrobiano e da viabilidade celular de diferentes concentrações de Ozonioterapia
(Universidade Brasil, 2022) Morais, Jynani Pichara; Assis, Lívia; Tim, Carla Roberta
Cutaneous wound is a clinical condition with high prevalence and represents a public health problem. Although ozone therapy (O3), ozone therapy, is widely used in clinical practice due to its antimicrobial and restorative effects, evidence on ideal dosimetries is still scarce. Thus, the aim of the present study was to evaluate and compare, in vitro, the cytotoxicity of different concentrations of ozone therapy in keratinocyte (NOK) and fibroblast (L929) lines, as well as the antimicrobial potential for Staphylococcus aureus (S.aureus) and Escherichia coli (E. coli). NOK and L929 cells were seeded at a concentration of 1x104 and strains of gram-positive bacteria S.aureus and gram negative bacteria E. coli at a concentration of 1x108 and divided into 7 experimental groups treated with ozone at doses of 0 (G0), 5 (G5), 10 (G10), 20 (G20), 30 (G30), 40 (G40) and 50 μg/mL (G50). The effect of ozone on cell viability/cytotoxicity was determined using the alamarBlue® assay, the Live/Dead® assay, and morphometric analysis of the percentage of live and dead cells. The effect of the gas on the microorganisms was evaluated by counting the colony forming unit (CFU). The results obtained show that ozone at concentrations of 5, 10 and 20 μg/mL presented the highest viability values of NOK (keratinocytes) and L929 (fibroblasts) cells, as well as a higher density and percentage of live cells in the experimental period of 72 hours when compared to other concentrations. However, it was not possible to observe the difference between the percentage of dead cells between the experimental groups. Furthermore, ozone promoted a reduction in CFUs at all concentrations tested. With the results obtained in the present study, it is possible to confirm the reparative potential of ozone therapy in a dose-dependent response, as well as its antimicrobial potential. However, studies are needed to elucidate the molecular mechanisms by which it exerts such biological effects.
Caracterização e testes clínicos de dermocosmético antimicrobiano para coberturas de feridas cutâneas de difícil reparo
(Universidade Brasil, 2022) Serra, Thallita Karollaine de Queiroz Pereira; Tim, Carla Roberta; Santos, Laurita dos
This study aimed to characterize an ozonized dermocosmetic and verify in clinical trials its antimicrobial and repairing potential in difficult-to-repair skin wounds. For the production of dermocosmetic, sunflower oil was used. The acid number, peroxide number, and iodine number were analyzed using The American Oil Chemists' Society method. The clinical test was developed with patients with difficult-to-repair wounds and approved by the ethics and research committee (nº 4, 246, 236). Then the patients were randomly allocated into Group 1, treatment with ozonized dermocosmetics, and Group 2, treatment with conventional dressing. Treatments were performed three times a week for 12 weeks. The characterization results showed that the dermocosmetic had an acid index of 17.68± 0.03 mg KOH/g, an iodine index of 86 ± 1 Cgl/g, and a peroxide index of 266.52 meq/kg. In the macroscopic evaluation after 12 weeks, an improvement in the appearance of the lesions was observed, such as an increase in granulation tissue, absence of odor, and a decrease in slough and presence of liquids. There was also complete repair in some injuries, so this group showed stimulation of the repair process with a reduction of 67 to 100% in the area of injury. Initially, 38 bacteria from 13 different species were identified in both groups. The bacteria were classified according to Gram staining. Thus, Gram-negative corresponds to 76.31%, with Pseudomonas aeruginosa being the most diagnosed. Gram-positives represent 23.69%, with Enterococcus faecalis being the most common. The identified microorganisms were evaluated using the antibiogram, divided into sensitive and resistant. Furthermore, it was observed that the use of the ozonized dermocosmetic promoted an antibacterial effect on the lesions of 13 patients. The ozonated dermocosmetic group had a higher frequency of patients without pain after treatment. However, in the conventional dressing group, after 12 weeks, there was a small change in the wounds without significant improvement. Only one patient showed the absence of microorganisms after the treatment period. Furthermore, 37.50% of the patients in the conventional dressing group had an increase in the lesion area, and the pain level was considered moderate. It is concluded that the dermocosmetic group promoted an antimicrobial effect and thus stimulated the repair process of cutaneous wounds.
Comportamentos e ações de vida diária e sua relação com a infecção pelo coronavírus
(Universidade Brasil) Carvalho, Jucilene Santos de; Assis, Lívia; Tim, Carla Roberta; Santos, Laurita dos
Efeito antibacteriano da água ozonizada sobre agentes etiológicos de doenças transmitidas por alimentos
(Universidade Brasil, 2024) Moraes, Carla Maria Zordan Geraldo de; Kozusny-Andreani, Dora Inés; Tim, Carla Roberta
Fresh vegetables have been frequently identified as a source of bacterial pathogens that can cause foodborne illnesses. Microbial pathogens can adhere and form biofilms on the surfaces of raw fruits and leafy vegetables and can be difficult to remove. Different chemical sanitizers are used for food sanitization, however ozone, due to its oxidative power, has a broad antibacterial effect on a variety of species, including spores, vegetative cells and Gram-positive and Gram-negative bacteria. Ozone is an important tool in vegetable sanitization and plays a relevant role in improving food quality and microbial safety, since the control of spoilage and pathogenic microorganisms is essential throughout the production chain of cultivation, processing, distribution and consumption. The aim of this study was to evaluate the efficacy of ozonated water in the inactivation of Salmonella Enterica serovar Typhimurium (TTC 14028) and Escherichia coli O157:H7 (ATCC 43888) in Sweet Grape tomatoes. To perform the experiments on bacterial viability in suspension and in inoculated tomatoes, the inoculum was prepared in tryptic soybean broth and incubated at 37 °C for 24 hours and adjusted to 106 CFU mL-1. The tomatoes were inoculated by placing 10 units in an autoclaved glass container containing 500 mL of the inoculum of each bacteria and gently shaken for 10 min, and deposited in a biosafety cabinet for 12 hours to facilitate bacterial fixation, subsequently they were removed and air-dried for 10 min in a biosafety cabinet. The ozonation of the water was produced by a commercial ozone generator operating with corona effect and fed by pure oxygen (1.0 L minute-1 ). The gas concentration limited by the calibrated equipment had an O3 flow rate of 17 mg. L-1 . The bacterial suspensions and the inoculated tomatoes were exposed to O3 directly by permanent bubbling at a controlled room temperature of 20ºC. Samples of 0.1 mL were collected at different time periods (0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes), and inoculated on eosin methylene blue agar and xylose lysine deoxycholate agar, incubated at 37°C for 24-48 hours when the colonies were counted. Descriptive statistics of the microbial counts were used for data analysis. The Analysis of Variance (ANOVA) test with Tukey's multiple comparison test was applied to verify possible significant differences between the microbial counts over the time of exposure of the tomatoes to ozonated water. Inactivation of Salmonella enterica serovar Typhimurium (ATCC 14028) and Escherichia coli O157:H7 (ATCC 43888) in suspension was observed in 90 minutes and 120 minutes, respectively. E. coli was more resistant to the action of ozonated water, since its microbial count was zero 15 minutes after exposure of the inoculated tomatoes. Meanwhile, tomatoes contaminated with S. typhimurium needed only 10 minutes of exposure to ozonated water to be considered free of contamination. The application of aqueous ozone in the dynamic mode showed antimicrobial effects on the inactivation rate of bacterial cells in suspension and attached (biofilm) to the surface of the tomatoes.
Efeito do selante de fibrina heterólogo associado ao exercício aquático no processo de reparo tendíneo em ratas
(Universidade Brasil, 2020) Hidd, Silvia Maria Cardoso Magalhães; Amaral, Marcelo Magri; Tim, Carla Roberta
Acute rupture of the calcaneus tendon is a very common type of injury, its increasing is generally related to sports practices. Its treatment is a controversial topic, involving several methods, conservative and surgical. These techniques can trigger problems that harms their complete regeneration. Currently, several studies have been carried out looking for new therapies to provide a low-cost fast tissue repair and reduced negative effects on population health conditions. Among different approaches, biomaterials have presented an excellent alternative for this regeneration process. Fibrin sealant derived from snake venom (SFDVS) has been used successfully in different surgical methods, both in animal’s and humans’ models, due to its hemostatic and adhesive potential. Aquatic exercise is one of the therapeutic strategies for rehabilitation, it consists of physical exercises to improve muscle properties, helping to reduce pain and edema, stimulating the repair process. Thus, the present study aimed to value the use of SFDVS associated or not with aquatic exercise as a form of treatment in the process of repair of the calcaneus tendon of rats. For this, 84 rats of the Wistar strain underwent surgical induction with partial transection of the calcaneus tendon. The animals were randomly divided into four treatment groups and subdivided into three periods (7.14 and 21 days) containing 7 animals in each: Control (L); SFDVS (LS); and aquatic exercise (LE); SFDVS associated with aquatic exercise (LSE). The volume of edema was evaluated 24 h, 7, 14 and 21 days after the surgical procedure. Histological sections of the calcaneus tendon were evaluated for quantification of collagen (Masson's Trichrome and Picrosirius Red) and pathological changes by the analysis of slides stained with HE. The Bonar score was used to evaluate cell morphology, cellularity, vascularization and accumulation of fundamental substance. The comparison between the experimental groups was performed using Kruskal-Wallis and ANOVA tests, with a significance level of 0.05. A greater reduction in animal edema was observed from the seventh day on in all treatments (p < 0.002), when compared to the control group. After 7 and 14 days of treatment, the LE presented the greatest reduction in the volume of edema (p = 0.03041) compared to the control. After 21 days of treatment (LS) it was the one that presented the greatest reduction of edema compared to the control group. By quantifying collagen, it was possible to verify an increase in its concentration for LSE not the period evaluated after 21 days of treatment. Histopathological analysis using Bonar score showed a significant improvement in histopathological characteristics in tendons of animals treated with (LSE21), presenting statistically lower score values when compared to the other groups. Thus, the SFDVS associated with aquatic exercise positively influenced the repair of the calcaneus tendon, becoming a promising treatment for future clinical applications.
Efeitos da fotobiomodulação associada ou não ao Biopolímero de Fibrina Heterólogo no processo de reparo tecidual de lesões cutâneas de ratos diabéticos
(Universidade Brasil, 2021) Alexandria, Francisco Eugênio Deusdará de; Assis, Lívia; Tim, Carla Roberta
INTRODUCTION: Diabetes mellitus is a general heterogeneous term that classifies metabolic disorders caused by increased glucose levels (hyperglycemia). Among the therapeutic modalities researched in order to accelerate tissue repair, LED photobiomodulation therapy and heterologous fibrin biopolymer stand out. PURPOSE: To evaluate the effects of LED photobiomodulation therapy associated or not with heterologous fibrin biopolymer in the tissue repair process of skin lesions in diabetic rats. METHODOLOGY: Forty-eight adult female Wistar rats (Rattus norvegicus albinus), 60 days old, weighing 230±20 g, from the vivarium of the State University of Piauí, were used. The animals were randomly divided into 4 distinct groups (control group, heterologous fibrin biopolymer group, LED photobiomodulation therapy group, LED photobiomodulation therapy group associated with heterologous fibrin biopolymer), which were treated in two experimental periods (7 and 14 days). Diabetes was induced by the administration of an aqueous solution of 2% alloxan and the animals had their blood glucose assessed using a glucometer and clinical symptoms. The animals underwent two surgical procedures, in the dorsal and cervical regions, the first by means of a skin punch, an area of 2 cm²; and the second a linear lesion measuring 3 cm, in the cervical region. Healing analysis was performed by calculating the contraction of the wound area and evaluating the traction force. RESULTS: The treatments with LED photobiomodulation therapy and heterologous fibrin biopolymer, used alone or in combination, indicated the stimulation of the repair process. The heterologous fibrin biopolymer group showed similar results to the LED photobiomodulation therapy group in wound regression, but showed better healing resistance, suggesting that the LED group has both repair qualities. The LED photobiomodulation therapy group associated with heterologous fibrin biopolymer showed better results in resistance and regression when compared to the heterologous fibrin biopolymer and LED photobiomodulation therapy groups, statistically closer to the control group. CONCLUSION: Based on the findings, it was observed that treatments with LED, BFH or LED associated with BFH acted satisfactorily, favoring the improvement in the tissue repair process, suggesting that these treatments, isolated or associated, were effective in stimulating the healing process in diabetic rats.
Efeitos da fotobiomodulação associada ou não ao Biopolímero de Fibrina Heterólogo no processo de reparo tecidual de lesões cutâneas de ratos diabéticos
(Universidade Brasil, 2021) Alexandria, Francisco Eugênio Deusdará de; Tim, Carla Roberta; Assis, Lívia
Efeitos do agulhamento seco no tratamento da dor de pacientes com disfunções temporomandibulares
(Universidade Brasil, 2021) Castro, Tânia Ecí Santi; Assis, Lívia; Tim, Carla Roberta
Temporomandibular disorder (TMD) is a disorder of the masticatory system originating from the masticatory muscles and/or the temporomandibular joint and associated structures. This dysfunction is responsible for the discomfort of millions of people around the world, decreasing their quality of life and restricting social interaction. In this perspective, this study aimed to identify the potential of dry needling in the treatment of TMD through a systematic literature review and evaluation of the use of deep dry needling for the treatment of TMD. For this, articles were searched in the PubMed and Virtual Health Library (LILACS, MEDLINE and SciELO) databases with the descriptors in English "temporomandibular disorder dry needling", or "temporomandibular disorder myofascial dry needling", or "temporomandibular disorder dry needling. Using a combination of descriptors as the search strategy. Nine studies were considered. One study compared superficial and deep dry needling. Five studies used only deep dry needling, another three studies did not specify the type of needling used, and only one article compared deep with superficial dry needling. of cases with three patients with chronic TMD diagnosed through clinical examination based on the Research Diagnostic Criteria for Temporomandibular Disorders (RDC / TMD) and undergoing 4 seasons needling dry, once a week. Deep dry needling was used in the g-points tile in the masseter muscles and through temporal needles 0.20 x 13 mm. After 4 weeks of treatment, it was observed that pain intensity and hospital anxiety and depression scale scores decreased and increased mouth opening. Based on the results, it can be suggested that dry needling promoted a remission of painful symptoms and an improvement in mandibular range of motion after four weeks of treatment.
Efeitos do agulhamento seco no tratamento da dor de pacientes com disfunções temporomandibulares
(Universidade Brasil, 2021) Castro, Tânia Ecí Santi; Tim, Carla Roberta
Temporomandibular disorder (TMD) is a disorder of the masticatory system originating from the masticatory muscles and/or the temporomandibular joint and associated structures. This dysfunction is responsible for the discomfort of millions of people around the world, decreasing their quality of life and restricting social interaction. In this perspective, this study aimed to identify the potential of dry needling in the treatment of TMD through a systematic literature review and evaluation of the use of deep dry needling for the treatment of TMD. For this, articles were searched in the PubMed and Virtual Health Library (LILACS, MEDLINE and SciELO) databases with the descriptors in English "temporomandibular disorder dry needling", or "temporomandibular disorder myofascial dry needling", or "temporomandibular disorder dry needling. Using a combination of descriptors as the search strategy. Nine studies were considered. One study compared superficial and deep dry needling. Five studies used only deep dry needling, another three studies did not specify the type of needling used, and only one article compared deep with superficial dry needling. of cases with three patients with chronic TMD diagnosed through clinical examination based on the Research Diagnostic Criteria for Temporomandibular Disorders (RDC / TMD) and undergoing 4 seasons needling dry, once a week. Deep dry needling was used in the g-points tile in the masseter muscles and through temporal needles 0.20 x 13 mm. After 4 weeks of treatment, it was observed that pain intensity and hospital anxiety and depression scale scores decreased and increased mouth opening. Based on the results, it can be suggested that dry needling promoted a remission of painful symptoms and an improvement in mandibular range of motion after four weeks of treatment.
Efeitos do biopolímero de fibrina heterólogo e fotobiomodulação no reparo de feridas cutâneas em ratos
(Universidade Brasil, 2021) Silva, Naldiana Cerqueira; Maia Filho, Antônio Luiz Martins; Tim, Carla Roberta
Cutaneous wounds are a public health problem in Brazil and worldwide with a negative impact on the lives of thousands of people, that raise treatment costs and make it difficult to maintain the sustainability of health systems. The aim of the present study was to evaluate the effect of LED-mediated-photobiomodulation therapy associated or not with the use of heterologous fibrin biopolymer (HFB) on the repair of cutaneous wounds in rats. Male Wistar rats were randomly divided into 4 groups of 21 animals as follows: control group (CG) whose wounds were treated with 0.9% saline solution; LED group (LED) whose wounds were irradiated with LED (660 nm, 166 mW, 60 s, 9.96 J); heterologous fibrin biopolymer group (HFB) whose wounds were treated exclusively with HFB; group LED + heterologous fibrin biopolymer (LED + HFB) whose wounds were irradiated with LED and HFB was applied. Two full-thickness cutaneous wounds were created on the back of each animal: a longitudinal wound measuring 3 cm and a circular wound with the diameter of 2 cm. The applications of LED and HFB were performed immediately after injury and followed an interval period of 72 hours between the posterior applications as described: with 2 applications for the 7-day groups, 4 applications for the 14-day groups and 6 applications for the 21-day groups. Animal euthanasia occurred in three different time periods: 7, 14 and 21 days after surgery. Macroscopic analysis, morphometric analysis of the wound repair index, descriptive histological and histomorphometric analysis, collagen quantification and tensile strength analysis. In the macroscopic findings, it was possible to observe the absence of necrosis, odor, presence of fibrosis or fluid in the lesion cavity in all experimental groups. Particularly In the control group, there was mild local inflammation evidenced between the fifth and sixth day after injury, that did not manifest in the other groups. The analysis of the wound repair index demonstrated that the treated groups presented greater wound reduction in the three periods of evaluation. Histopathological analysis revealed that the treatments presented a better repair process in comparison to the control group. Comparing the applied treatments, it was observed that only in the HFB group the dermis exhibited a denser extracellular matrix after 21 days. The collagen evaluation demonstrated that all treatments induced greater collagen deposition and maturation when compared to the control group. However, at 14 and 21 days the HFB group presents greater deposition of collagen type I and collagen maturation. The analysis of the traction force at 7 days of experiment showed better performance of the treated groups, while at 14 and 21 days the group that used HFB had greater resistance in the suture region. The results indicate that treatments using HFB and LED, either isolated or associated, stimulated the wound repair process in rats.
Efeitos do Biopolímero de fibrina heterólogo e fotobiomodulação no reparo de feridas cutâneas em ratos
(Universidade Brasil, 2021) Silva, Naldiana Cerqueira; Tim, Carla Roberta; Maia Filho, Antônio Luiz Martins
Cutaneous wounds are a public health problem in Brazil and worldwide with a negative impact on the lives of thousands of people, that raise treatment costs and make it difficult to maintain the sustainability of health systems. The aim of the present study was to evaluate the effect of LED-mediated-photobiomodulation therapy associated or not with the use of heterologous fibrin biopolymer (HFB) on the repair of cutaneous wounds in rats. Male Wistar rats were randomly divided into 4 groups of 21 animals as follows: control group (CG) whose wounds were treated with 0.9% saline solution; LED group (LED) whose wounds were irradiated with LED (660 nm, 166 mW, 60 s, 9.96 J); heterologous fibrin biopolymer group (HFB) whose wounds were treated exclusively with HFB; group LED + heterologous fibrin biopolymer (LED + HFB) whose wounds were irradiated with LED and HFB was applied. Two full-thickness cutaneous wounds were created on the back of each animal: a longitudinal wound measuring 3 cm and a circular wound with the diameter of 2 cm. The applications of LED and HFB were performed immediately after injury and followed an interval period of 72 hours between the posterior applications as described: with 2 applications for the 7-day groups, 4 applications for the 14-day groups and 6 applications for the 21-day groups. Animal euthanasia occurred in three different time periods: 7, 14 and 21 days after surgery. Macroscopic analysis, morphometric analysis of the wound repair index, descriptive histological and histomorphometric analysis, collagen quantification and tensile strength analysis. In the macroscopic findings, it was possible to observe the absence of necrosis, odor, presence of fibrosis or fluid in the lesion cavity in all experimental groups. Particularly In the control group, there was mild local inflammation evidenced between the fifth and sixth day after injury, that did not manifest in the other groups. The analysis of the wound repair index demonstrated that the treated groups presented greater wound reduction in the three periods of evaluation. Histopathological analysis revealed that the treatments presented a better repair process in comparison to the control group. Comparing the applied treatments, it was observed that only in the HFB group the dermis exhibited a denser extracellular matrix after 21 days. The collagen evaluation demonstrated that all treatments induced greater collagen deposition and maturation when compared to the control group. However, at 14 and 21 days the HFB group presents greater deposition of collagen type I and collagen maturation. The analysis of the traction force at 7 days of experiment showed better performance of the treated groups, while at 14 and 21 days the group that used HFB had greater resistance in the suture region. The results indicate that treatments using HFB and LED, either isolated or associated, stimulated the wound repair process in rats.
Fotobiomodulação como coadjuvante no tratamento da lesão pulmonar aguda decorrente de sepse
(Universidade Brasil, 2020) Dantas, Emanuelle; Garcia, Lívia Assis; Tim, Carla Roberta
Sepsis is a common condition associated with high mortality and, for many who survive, long-term morbidity. It is defined as infection with organ dysfunction, with the lung being one of Organs most affected organs. Acute lung injury (ALI) is a serious and serious disease, characterized by infiltrations of pulmonary inflammatory cells, diffuse alveolar damage, in addition to loss of the alveolar epithelium, together with edema and changes in gas exchange. During the rehabilitation period, there is a growing concern to modulate these processes in order to prevent complications and favor the patient's functional recovery. The therapeutic verification of the applications of photobiomodulation therapies (PBM) through lasers and LEDs) in the clinical treatment of inflammatory diseases is currently well known, however the mechanisms of action triggered by these resources and their use in clinical practice for the treatment of APL due to sepsis still remains incipient. Thus, this thesis aimed to provide an understanding of the current evidence on the importance of PBM in the treatment of respiratory disorders and its possible applicability in APL due to sepsis, as well as to evaluate and compare in vivo the PBM response through laser and LEDs on the LPA in rats. A qualitative, descriptive and exploratory narrative literature review was carried out and an experimental study using male Wistar rats, allocated in 3 (three) experimental groups: Sepsis control (CS); laser treatment (LASER); LED treatment (LED). Irradiations in the red wavelength (630 nm) were applied at three points (anterior region of the trachea and in the ventral regions of the chest, bilaterally) 1 and 24 hours after surgery. Based on all verified evidence and results observed in this study, it is inferred that PBM through laser and LED can be proposed as a support for conventional medical therapy in the treatment of APL arising from sepsis, due to the potential to mitigate the inflammatory condition, prevent the progression of the lung parenchyma lesion and, consequently, reduce the recovery time of the patient with ALI due to sepsis.