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URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/30
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Resultados da Pesquisa
Item Uso da Ozonioterapia em fungos causadores de Onicomicoses: estudo in vitro(Universidade Brasil, 2024) Del Castilo, Denise Vivianni Ferreira; Assis, Lívia; Tim, Carla Roberta: Onychomycosis is a common fungal infection of the nails, predominantly caused by dermatophytes such as Trichophyton rubrum (T. rubrum) and Microsporum gypseum (M. gypseum) and is a notoriously difficult condition to treat. Thus, the objective of the present study was to evaluate and compare, in vitro, the effectiveness of different times of ozone therapy on the main fungi that cause onychomycosis. Dermatophyte fungi T. rubrum (ATCC 28188) and M. gypseum (ATCC 24102) were incubated at 28° C for 14 days. Subsequently, the fungi were divided into 9 experimental groups according to the experimental time: Control Group (CG): the fungi were cultivated and did not receive any treatment; Cultivated fungi and received ozone therapy for a time of 2 min (G2”); 4 min (G4”); 6 min (G6”); 8 min (G8”); 10 min (G10”); 12 min (G12”); 14 min (G14”) and 16 min (G16”). The ozone concentration used was 2 µg/mL, oxygen flow of 1/4 L/min and applied dosages of 157, 314, 451, 628, 785, 943, 1100 and 1257 mg/m2, respectively. The main results show that the use of topical ozone therapy was effective in reducing the germination percentage of T. rubrum and M. gypseum in all experimental periods, presenting complete eradication with a treatment time of 6 min for T. rubrum and 14 min for M. gypseum. In conclusion, ozone therapy used topically, with emphasis on the treatment time of 6 min for T. rubrum (628 mg/m2) and 14 min (1257 mg/m2) for M. gypseum, promoted antifungal action on the main dermatophytes responsible for critical complications of onychomycosis, and may be proposed as a adjuvant in dermatological treatments.Item Avaliação da eficácia de óleos vegetais in natura e ozonizados no controle de cândida spp.(Universidade Brasil, 2022) Gimenez, Vanessa Barbosa; Kozusny-Andreani, Dora InésCandida spp, is a pathogen associated with health, causes superficial or invasive infections with a high mortality rate. The pathogenicity is attributed to virulence factors such as adhesion, proteinases, phospholipases, and hemolysins. The emergence of strains resistant to conventional treatments, highlights the need to obtain highly effective drugs that do not generate microbial resistance, among which, ozonized vegetable oils have gained prominence for their wide range of antimicrobial effect, are effective in antifungal therapies, antibacterial and antiviral. In this context, the objective of this research was to evaluate the effectiveness of ozonated and in natura vegetable oils in the in vitro inactivation of Candida albicans ATCC 25923 and Candida tropicalis ATCC 4563. To analyze the antifungal activity, vegetable oils of coconut, palm oil, sunflower, olive and pequi, all fresh and ozonized in concentrations ranging from 0.32% to 100%. The minimum inhibitory concentration was determined by the plate microdilution method, and then the minimum fungicidal concentration and the fungicidal kinetics of the oils were verified. The results showed that the vegetable oils of sunflower, coconut, palm oil, pequi and olive, in natura and ozonized, inactivated Candida tropicalis and C. albicans, however the in natura oils of palm, sunflower and coconut presented minimum inhibitory and fungicidal concentrations higher than those of ozonized oils, while those of olive and pequi did not show differences. Regarding the ability to reduce colony-forming units as a function of time, it was found that olive and pequi oils, ozonated and in natura, were more effective. Based on the results, the vegetable oils indicate the possibility of using them in the therapy of Candida spp.Item Efeito antibacteriano do óleo de girassol ozonizado em diferentes concentrações(Universidade Brasil, 2021) Ficher, Ester; Nunez, Silvia CristinaThe world is currently facing a crisis regarding microorganisms and microbial resistance. The evaluation of alternative antimicrobial therapies has been a constant search in recent decades. Ozonized oil can be an alternative within this field. The present study aims to analyze the antimicrobial efficacy of commercially available ozonated sunflower oil in different concentrations. A gram-positive bacteria Staphylococcus aureus and the gram-negative bacteria Escherichia coli were used. The microorganisms were grown in BHI broth (Brain and Heart Infusion) for 24h. Plates containing BHI agar were prepared for the inhibition halo test with a 3 mm diameter central well and contaminated with 50 µL of bacterial suspension. The wells made in the plates were filled with ozonated sunflower oil in concentrations ranging from 80 mmol-Eq / kg to 1290 mmol-Eq /. The plates were taken to the incubator at 37°C for 24h and the inhibition zone was measured with a millimeter probe, two control groups one with non-ozonated sunflower oil and one with 2% chlorhexidine gluconate solution were also evaluated. The results demonstrated that the efficacy for E.coli increases linearly with increasing concentration, however, only the concentrations of 702 mmol-Eq / kg and 1290 mmol-Eq / kg were significantly different (p<0.004). For S.aureus, the results showed significant differences only in the concentration of 1290 mmol-Eq / kg, but with a similar effect between the intermediate and higher concentrations (p<0.0001). The chlorhexidine group demonstrated to have a more effective action for both microorganisms under the conditions of this study (p<0.001). According to the results ozonized sunflower oil has less antimicrobial effect than chlorhexidine, the result is dependent on the concentration and only concentrations above 702 mmol-Eq / kg showed some efficacy.Item Influência do tempo de ozonólise no processo de ozonização de óleo vegetal utilizado para o reparo de pele(Universidade Brasil, 2023) Tarocco, Juliana Carolina; Tim, Carla Roberta; Santos, Laurita dosLesões cutâneas, que são interrupções na continuidade das camadas da pele, afetam significativamente a qualidade de vida tanto de humanos quanto de animais. Essas lesões também implicam em altos custos para a rede de saúde pública e gastos consideráveis em clínicas e hospitais veterinários. Dado esse contexto, este estudo objetivou avaliar a influência do tempo de ozonólise durante o processo de ozonização do óleo de girassol com fluxo de O2 de 3L/min em uma concentração de O3 = 41,6 g/m3 no estímulo do reparo de feridas dérmicas em animais. Foram utilizados 35 ratos Wistar machos, que tiveram feridas induzidas por um punch de 10 mm. Estes foram distribuídos em grupos: controle (sem tratamento), e grupos tratados com óleo de girassol ozonizado por 30, 60, 90 e 120 minutos. Em cada lesão, foram administrados 80 uL do óleo ozonizado topicamente por 10 dias consecutivos. Na avaliação macroscópica, nenhum animal apresentou sinais de odor, necrose, fibrose ou presença de líquido na lesão. A análise do índice de reparo indicou que todos os grupos tiveram a mesma redução percentual da área lesionada. Similarmente, a análise histológica não mostrou diferenças no processo de reparo entre os grupos. A espectroscopia Raman diferenciou com sucesso a pele lesionada da pele saudável, mas não conseguiu distinguir entre o grupo controle e os grupos tratados com óleo ozonizado. Conclui-se que o tempo de ozonização do óleo, até 120 minutos, não alterou de forma significativa as estruturas químicas da pele nem estimulou seu reparo.Item Uso de um gerador de ozônio de volume controlado na adiposidade localizada abdominal: ensaio clínico controlado e randomizado(Universidade Brasil, 2024) Silva, Fabiele Chieregato Marchetti da; Garcia, Lívia Assis; Tim, Carla RobertaOzone therapy has been suggested in clinical practice as a promising and adjuvant therapy in the minimally invasive treatment of localized adiposity; however, evidence is limited for its recommendations. Thus, the aim of the study was to evaluate the safety and efficacy of a controlled-volume ozone generator at different dosages in localized abdominal adiposity. To achieve this objective, a single-center randomized controlled clinical trial was conducted. Female participants were recruited and 110 were randomized into 4 groups: Sham Group (GS): participants received treatment with the ozone equipment turned off; Intervention Groups: participants received ozone treatment at doses of 20 (G20), 30 (G30), and 40 g/ml (G40). The interventions were performed at 24 pre-established points in the abdominal region, twice a week for a period of 4 weeks, totaling 8 sessions. The initial assessment (A1) and reassessment [15 (A2) and 30 (A3) days after the last session] were performed through anthropometric analyses, bioimpedance, abdominal circumference, adipometry, global aesthetics (GAIS), adipose tissue thickness through diagnostic ultrasound, recording of patient satisfaction assessment (Linkert) and adverse effects. The main results show that groups G20, G30 and G40 presented a reduction in abdominal circumference in the periods of 15 and 30 days after treatment, lower GAIS score and improvement in the treatment satisfaction score in both periods evaluated. However, G40 presented lower adipose tissue thickness in both experimental periods. No serious adverse effects were reported. Thus, it can be suggested that the controlled volume ozone generator used in the present study proved to be a safe and potentially promising modality in the treatment of localized adiposity, with emphasis on the dose of 40 ug/ml.Item Ozonização de Castanhas de Caju e do Brasil para o controle de fungos filamentosos(Universidade Brasil, 2020) Souza, Midian Nikel Alves de; Frias, Danila Fernanda RodriguesThe contamination of chestnuts by filamentous fungi, which produce mycotoxins, is considered a serious public health problem, which makes its control essential to obtain a quality and safe product for the consumer. Thus, the objective of this research was to evaluate the fungicidal effect of ozone against filamentous fungi isolated from cashew nuts and Brazil nuts. For this, 10 samples of chestnuts commercialized in bulk were used in the municipality of Fernandópolis – SP, from october to december, 2019. The search for filamentous fungi was carried out using the serial dilution method and plating on Potato Dextrose Agar. The dishes were incubated at 25ºC for 5 days, then the colony counts and genera identification were performed by observing the macroscopic characteristics and micromorphological structures. To test the effectiveness of ozone, 25g of chestnuts were diluted in 225 mL of 1% peptone water. This material was exposed to ozone at different times (5, 10, 15, 20, 25 and 30 minutes). In each time interval, 0,1 mL aliquots were inoculated in triplicate, in Petri dishes containing Potato Dextrose Agar, to determine the minimum fungicidal concentration (MFC). After tabulation of the collected data, two statistical analysis functions were performed: descriptive and inferential. The data were replicated in an absolute and relative way. In the inferential scope, the test Paired T Test was used. The fungal microbiota was detected in all samples analyzed, and in 70% of these, more than one genus was isolated. The genera were isolated: Aspergillus, Penicillium, Cladosporium, Curvularia, Absidia and Rhizopus. With the use of ozone, an average reduction of 96.5% of fungi can be seen in up to 30 minutes of exposure. It was concluded that ozonation was efficient in inactivating filamentous fungi and, consequently, in preventing mycotoxin synthesis.Item Efeito de óleos essenciais in natura e ozonizados sobre o desenvolvimento de fungos patogênicos(Universidade Brasil, 2020) Kume, Joelma Évelin Pereira; Kozusny-Andreani, Dora InésSome species of fungi are capable of causing mycotic infections. One of these infections is dermatophytosis, caused by a group of fungi, called dermatophytes. another infection is sporotrichosis, caused by species of Prothorax schenckii. The treatment of the disease is carried out using conventional antifungals. The emergence of resistant strains has led to alternative treatments, such as natural medicines or the use of ozone gas. The objective of this research was to evaluate the antifungal activity of fresh and ozonated essential oils against Trichophyton mentagrophytes, Trichophyton rubrum, and Sporothrix schenckii. In natura and ozonized essential oils of Cinnamomum cassia, Eugenia Caryphollata, Cymbopogon winterianus, Eucalyptus globulus, Eucalyptus staigeriana and Mentha piperita were used, evaluated for antifungal activity against the microorganism lineage. The oils were ozonized in corona equipment. The microdilution technique was used to assess the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC). The fungicidal kinetics of essential oils were determined. The data obtained were evaluated by the Mann-Whitney test and by the east of Kruskal-Wallis. The fungus T. mentagrophytes was more resistant. T. rubrum showed less resistance to treatments, showing decreases in microbial count in the first moments of exposure. The Spothrix fungus showed greater resistance to cinnamon, clove and Eucalyptus staigeriana oils, showing a decrease in microbial count variation. In general, the results show the possibility of use in antifungal therapy against the studied microorganisms.Item Efeito antibacteriano do óleo de girassol ozonizado em diferentes concentrações(Universidade Brasil, 2021) Ficher, Ester; Nuñez, Silvia CristinaThe world is currently facing a crisis regarding microorganisms and microbial resistance. The evaluation of alternative antimicrobial therapies has been a constant search in recent decades. Ozonized oil can be an alternative within this field. The present study aims to analyze the antimicrobial efficacy of commercially available ozonated sunflower oil in different concentrations. A gram-positive bacteria Staphylococcus aureus and the gram-negative bacteria Escherichia coli were used. The microorganisms were grown in BHI broth (Brain and Heart Infusion) for 24h. Plates containing BHI agar were prepared for the inhibition halo test with a 3 mm diameter central well and contaminated with 50 µL of bacterial suspension. The wells made in the plates were filled with ozonated sunflower oil in concentrations ranging from 80 mmol-Eq / kg to 1290 mmol-Eq /. The plates were taken to the incubator at 37°C for 24h and the inhibition zone was measured with a millimeter probe, two control groups one with non-ozonated sunflower oil and one with 2% chlorhexidine gluconate solution were also evaluated. The results demonstrated that the efficacy for E.coli increases linearly with increasing concentration, however, only the concentrations of 702 mmol-Eq / kg and 1290 mmol-Eq / kg were significantly different (p<0.004). For S.aureus, the results showed significant differences only in the concentration of 1290 mmol-Eq / kg, but with a similar effect between the intermediate and higher concentrations (p<0.0001). The chlorhexidine group demonstrated to have a more effective action for both microorganisms under the conditions of this study (p<0.001). According to the results ozonized sunflower oil has less antimicrobial effect than chlorhexidine, the result is dependent on the concentration and only concentrations above 702 mmol-Eq / kg showed some efficacy.