Efeito antibacteriano do óleo de girassol ozonizado em diferentes concentrações
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Data
2021
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Universidade Brasil
Resumo
The world is currently facing a crisis regarding microorganisms and microbial
resistance. The evaluation of alternative antimicrobial therapies has been a constant
search in recent decades. Ozonized oil can be an alternative within this field. The
present study aims to analyze the antimicrobial efficacy of commercially available
ozonated sunflower oil in different concentrations. A gram-positive bacteria
Staphylococcus aureus and the gram-negative bacteria Escherichia coli were used.
The microorganisms were grown in BHI broth (Brain and Heart Infusion) for 24h.
Plates containing BHI agar were prepared for the inhibition halo test with a 3 mm
diameter central well and contaminated with 50 µL of bacterial suspension. The wells
made in the plates were filled with ozonated sunflower oil in concentrations ranging
from 80 mmol-Eq / kg to 1290 mmol-Eq /. The plates were taken to the incubator at
37°C for 24h and the inhibition zone was measured with a millimeter probe, two
control groups one with non-ozonated sunflower oil and one with 2% chlorhexidine
gluconate solution were also evaluated. The results demonstrated that the efficacy
for E.coli increases linearly with increasing concentration, however, only the
concentrations of 702 mmol-Eq / kg and 1290 mmol-Eq / kg were significantly
different (p<0.004). For S.aureus, the results showed significant differences only in
the concentration of 1290 mmol-Eq / kg, but with a similar effect between the
intermediate and higher concentrations (p<0.0001). The chlorhexidine group
demonstrated to have a more effective action for both microorganisms under the
conditions of this study (p<0.001). According to the results ozonized sunflower oil has
less antimicrobial effect than chlorhexidine, the result is dependent on the
concentration and only concentrations above 702 mmol-Eq / kg showed some
efficacy.
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Palavras-chave
Antimicrobianos, Resistência microbiana, Ozonoterapia, Ozônio, Bioengenharia