Navegando por Autor "Santos, Laurita dos"

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Avaliação dos níveis de ansiedade em estudantes universitários
(Universidade Brasil, 2021) Meneses, Aline Martins Diolindo; Santos, Laurita dos; Amaral, Marcelo Magri
University admission is considered an anxiety generating experience to students due to the educational method transition stage from High School to University. Anxiety is a psychopathology incident in University students in which the symptomatology affects the formal learning construction, generating psychological illness. Scientific studies show a higher incidence of anxiety disorders in the health field students compared to the general population, pointing out the need for IES’ to evaluate the students’ psychological conditions, offering support services. Goal: Assess the anxiety signs and symptoms and its correlation with sociodemographic and lifestyle characteristics in University students. Methods: It is about an analytical and crosssectional study, conducted with 120 University students. The data was collectedthrough the following instruments: Sociodemographic and lifestyle questionnaire, BeckAnxiety Inventory (BAI) and saliva collection and salivary cortisol dosage. The researchwas approved by the Ethics and Research Committee of Universidade Brasil, under the opinion number 3.732.897. Results: A total of 120 students were a part of the study,106 being female and 14 being male students. The average age was 26.4 years old (Median:23.00; Standard deviation: 9.174; Min: 18; Max: 55). Out of the students, 77.5% are single and 76.7% have no kids. For the use of BAI, 16.7% of students had minimal anxiety, 13.3% had mild anxiety, 16.6% moderate anxiety and 53.3% severe anxiety. Regarding the dosage of salivary cortisol in the collected samples, 89.2% of students were within standard limits (under 0.736 µg / dL); 7.8% of students had cortisolvalues above the reference values. Conclusion: The data collected in the studyrevealeda high occurrence of students with signs and symptoms of anxiety disorders.The results reinforce the need for IES’ to include actions to identify students in psychological distress in their practices, concomitant with the adoption of pedagogicalpractices that envision their mental health.
Avaliação dos níveis de ansiedade em estudantes universitários
(Universidade Brasil, 2021) Meneses, Aline Martins Diolindo; Santos, Laurita dos; Amaral, Marcelo Magri
Introduction: University admission is considered an anxiety generating experience to students due to the educational method transition stage from High School to University. Anxiety is a psychopathology incident in University students in which the symptomatology affects the formal learning construction, generating psychological illness. Scientific studies show a higher incidence of anxiety disorders in the health field students compared to the general population, pointing out the need for IES’ to evaluate the students’ psychological conditions, offering support services. Goal: Assess the anxiety signs and symptoms and its correlation with sociodemographic and lifestyle characteristics in University students. Methods: It is about an analytical and cross sectional study, conducted with 120 University students. The data was collectedthrough the following instruments: Sociodemographic and lifestyle questionnaire, BeckAnxiety Inventory (BAI) and saliva collection and salivary cortisol dosage. The researchwas approved by the Ethics and Research Committee of Universidade Brasil, under the opinion number 3.732.897. Results: A total of 120 students were a part of the study,106 being female and 14 being male students. The average age was 26.4 years old (Median:23.00; Standard deviation: 9.174; Min: 18; Max: 55). Out of the students, 77.5% are single and 76.7% have no kids. For the use of BAI, 16.7% of students had minimal anxiety, 13.3% had mild anxiety, 16.6% moderate anxiety and 53.3% severe anxiety. Regarding the dosage of salivary cortisol in the collected samples, 89.2% of students were within standard limits (under 0.736 µg / dL); 7.8% of students had cortisolvalues above the reference values. Conclusion: The data collected in the studyrevealeda high occurrence of students with signs and symptoms of anxiety disorders.The results reinforce the need for IES’ to include actions to identify students in psychological distress in their practices, concomitant with the adoption of pedagogicalpractices that envision their mental health.
Caracterização cardíaca em serpentes da espécie Pantherophis guttatus usando variabilidade da frequência cardíaca
(Universidade Brasil, 2021) Silva Neto, Antonio Gomes da; Santos, Laurita dos; Cogo, José Carlos
The use of electrocardiogram in reptiles can be an tool to monitor cardiac function in reptiles, however this technique is not broadly used, due to a limited understanding of its interpretation. This study analyzed the Heart Rate Variability (HRV) in Pantherophis guttatus snakes; animals that are endemic to the United States but distributed globally as a pet. Several cardiac diseases have been described for reptiles, however, for the studied snakes, these diseases are rarely diagnostic and in most cases the information is only based on the postmortem analysis. The use of linear HRV methods can contribute to the understanding of cardiac physiology, providing information for the diagnosis and prognosis of heart disease in snakes. Twenty-one snakes were used for the study (female=8, male=13) the snakes were not sedated. HRV parameters were made based on electrocardiographic records (ECG) and the RR intervals record were analyzed by non-linear methods, in the time and frequency domains. Significant differences were observed in the RR intervals for female and male snakes, varying by 18.8% and 15.3%, frequency. For the frequency of domain, the values varied between high and low. The changes observed in the time domain can be explained for the control of the autonomic nervous system by the action of the parasympathetic system, which is confirmed by the variations in the frequency domain. Even though significant differences were observed for cardiological variables between males and females, there is no way to infer the existence of pathological processes in any of the groups of snakes. To the best of our knowledge, this is the first time that the values established for SD1 and SD2, as well as the values of the frequency domain, with emphasis on the LF widely used in study in other species, were used to evaluate the cardiac function in Pantherophis.guttatus snakes.
Caracterização cardíaca em serpentes da espécie Pantherophis Guttatus usando variabilidade da frequência cardíaca
(Universidade Brasil, 2021) Silva Neto, Antonio Gomes da; Cogo, José Carlos; Santos, Laurita dos
The use of electrocardiogram in reptiles can be an tool to monitor cardiac function in reptiles, however this technique is not broadly used, due to a limited understanding of its interpretation. This study analyzed the Heart Rate Variability (HRV) in Pantherophis guttatus snakes; animals that are endemic to the United States but distributed globally as a pet. Several cardiac diseases have been described for reptiles, however, for the studied snakes, these diseases are rarely diagnostic and in most cases the information is only based on the postmortem analysis. The use of linear HRV methods can contribute to the understanding of cardiac physiology, providing information for the diagnosis and prognosis of heart disease in snakes. Twenty-one snakes were used for the study (female=8, male=13) the snakes were not sedated. HRV parameters were made based on electrocardiographic records (ECG) and the RR intervals record were analyzed by non-linear methods, in the time and frequency domains. Significant differences were observed in the RR intervals for female and male snakes, varying by 18.8% and 15.3%, frequency. For the frequency of domain, the values varied between high and low. The changes observed in the time domain can be explained for the control of the autonomic nervous system by the action of the parasympathetic system, which is confirmed by the variations in the frequency domain. Even though significant differences were observed for cardiological variables between males and females, there is no way to infer the existence of pathological processes in any of the groups of snakes. To the best of our knowledge, this is the first time that the values established for SD1 and SD2, as well as the values of the frequency domain, with emphasis on the LF widely used in study in other species, were used to evaluate the cardiac function in Pantherophis.guttatus snakes.
Caracterização de Eletroencefalograma utilizando análise de quantificação da recorrência
(Universidade Brasil, 2020) Mendes, Antônio de Pádua; Santos, Laurita dos; Rybarczyk Filho, José Luiz
The application of techniques for processing, analyzing and extracting features of electroencephalographic signals collaborate in understanding brain activities. Based on this, we can also mention the creation of diagnostic and monitoring tools allowing the development of brain-computer interface devices. Among the diagnostic tools there are several mathematical methods that can be considered, such as, the spectral analysis of the signal using the Fast Fourier Transform (FFT). In this study we investigated the applicability of a non-linear method already known for the analysis of biomedical signals and its comparison with a conventional analysis method, the FFT. The recurrence quantification analysis (RQA) was used as a method to characterize the alpha rhythm of electroencephalogram signals during the Berger effect (eyes closed). Electroencephalogram signals were obtained from 60 study participants, of both sexes, were analyzed using the electrodes of the occipital region (O1 and O2). The experimental protocol involved two distinct moments: rest (eyes open) and activity (eyes closed). For the application of the non-linear method, the optimized value for the parameters (time delay, embedding dimension and threshold) were obtained for classification. After parameterization, 9 measurements were obtained: recurrence rate, determinism, average of diagonal line, Shannon entropy, laminarity, trapping time, clustering coefficient, transitivity and maximum size of vertical lines. The results found were compared with results of the FFT, which considered as the gold standard of analysis. This combination was performed by multivariate analysis of principal components analysis. The results suggested that the use of RQA is capable of detecting significant statistical differences between the moments studied and that some RQA features may contribute to the analysis provided by the FFT. Thus, the RQA method can be considered for the analysis of alpha rhythm during the Berger effect. For future work, other brain regions can be studied and the methodology extended to analyze other brain waves during the performance of more complex activities.
Caracterização de óleo de girassol ozonizado em diferentes tempos usando Espectroscopia de Infravermelho com Transformada de Fourier
(Universidade Brasil, 2021) Uebele, Daniela; Santos, Laurita dos; Pessoa, Rodrigo Sávio
The estimated number of patients in Brazil and abroad, emphasizes the need for better monitoring and treatment of skin lesions, caused by numerous health problems, in addition to worsening by viral infections, bacteria, fungi and protozoa - the prospect is of increasing cases in developing and underdeveloped countries. This is due to the inefficient treatments offered by hospitals, with an emphasis on public hospitals. These are more than enough reasons to invest time in the analysis of an affordable therapeutic treatment compared to the current ones - such as the use of "ozone therapy". This is recognized and used on 5 continents and is even encouraged and sponsored by the governments and health plans of many countries. One of the safest ways of using ozone in treatments is through the by-products generated in the ozonolysis of vegetable oils, which can act on the skin, leading to the disinfection process, by cell lysis. In this work, the choice was for sunflower oil due to its physicochemical characteristics. The objective is to analyze the chemical structure of ozonized sunflower oil at different times over 90 minutes, without using the temperature control of the ozonolysis reactor. For that, the process of analysis by Fourier transform spectroscopy (FTIR) was used. 9 spectra were collected and analyzed by the deconvolution process, in comparison to the main bands observed by the second derivative. The results show that there was an increase in oil temperature (around 10ºC) during ozonation up to 30min. Three regions of the obtained spectra were analyzed: 3050-2800cm-1 , 1130-1070cm-1 and 1800-800cm-1 . Statistical results show a significant difference between spectral signals, with observance after the time of 20min of the sunflower oil ozonation process, with emphasis on the 1800–1500cm 1 region, which demonstrates the rupture of the double carbon bonds (C=C), breaking the lipid chain, and increasing the CO2 chain and thus reducing HC=CH, observed in the analysis of the spectra. There was a reduction in the intensity of the -CH band of the stretching of the -C=CH fragment above the 3000cm-1 region, an increase in the CH stretching bands of the CH3 groups, a reduction in the intensity of the -C=C- binding stretch and constant intensity of the band stretch -C, and all changes detected by FTIR are in accordance with the Criegee mechanisms. With these observations it can be reiterated how useful FTIR spectroscopy is for the analysis of changes in compounds. Following the studies with the support of collaborators, initial tests of the application of the samples of the ozonized sunflower oil were carried out, extending up to 120 minutes, in cutaneous lesions in ex-vivo in an animal model, using the same analysis process, observing whether there were changes between the spectra. In the statistical analysis, these changes are subtle, not enough to obtain results. For this, it is necessary to analyze in a wider region of the spectrum. It was not possible to obtain evidence of its benefits in the healing process. In need of in-depth studies, considering other factors relevant to the study.
Caracterização e testes clínicos de dermocosmético antimicrobiano para coberturas de feridas cutâneas de difícil reparo
(Universidade Brasil, 2022) Serra, Thallita Karollaine de Queiroz Pereira; Tim, Carla Roberta; Santos, Laurita dos
This study aimed to characterize an ozonized dermocosmetic and verify in clinical trials its antimicrobial and repairing potential in difficult-to-repair skin wounds. For the production of dermocosmetic, sunflower oil was used. The acid number, peroxide number, and iodine number were analyzed using The American Oil Chemists' Society method. The clinical test was developed with patients with difficult-to-repair wounds and approved by the ethics and research committee (nº 4, 246, 236). Then the patients were randomly allocated into Group 1, treatment with ozonized dermocosmetics, and Group 2, treatment with conventional dressing. Treatments were performed three times a week for 12 weeks. The characterization results showed that the dermocosmetic had an acid index of 17.68± 0.03 mg KOH/g, an iodine index of 86 ± 1 Cgl/g, and a peroxide index of 266.52 meq/kg. In the macroscopic evaluation after 12 weeks, an improvement in the appearance of the lesions was observed, such as an increase in granulation tissue, absence of odor, and a decrease in slough and presence of liquids. There was also complete repair in some injuries, so this group showed stimulation of the repair process with a reduction of 67 to 100% in the area of injury. Initially, 38 bacteria from 13 different species were identified in both groups. The bacteria were classified according to Gram staining. Thus, Gram-negative corresponds to 76.31%, with Pseudomonas aeruginosa being the most diagnosed. Gram-positives represent 23.69%, with Enterococcus faecalis being the most common. The identified microorganisms were evaluated using the antibiogram, divided into sensitive and resistant. Furthermore, it was observed that the use of the ozonized dermocosmetic promoted an antibacterial effect on the lesions of 13 patients. The ozonated dermocosmetic group had a higher frequency of patients without pain after treatment. However, in the conventional dressing group, after 12 weeks, there was a small change in the wounds without significant improvement. Only one patient showed the absence of microorganisms after the treatment period. Furthermore, 37.50% of the patients in the conventional dressing group had an increase in the lesion area, and the pain level was considered moderate. It is concluded that the dermocosmetic group promoted an antimicrobial effect and thus stimulated the repair process of cutaneous wounds.
Comportamentos e ações de vida diária e sua relação com a infecção pelo coronavírus
(Universidade Brasil) Carvalho, Jucilene Santos de; Assis, Lívia; Tim, Carla Roberta; Santos, Laurita dos
COVID-19 e os mecanismos de infecção relacionados aos hábitos e estilo de vida
(Universidade Brasil, 2022) Carvalho, Jucilene Santos de; Santos, Laurita dos
The COVID-19 pandemic presented itself as a major public health problem for Brazil and the world. It had an impact on health indicators such as morbidity, mortality and health expenditures, and important economic impacts related to the closure of establishments and social isolation. Given this scenario, this research was proposed for the municipality of São Caetano de Sul with the objective of analyzing the relationship between behaviors and life habits of people with coronavirus infection, and comparing the data between two specific moments of circulation of different variants. – Delta and Omicrôn. The data presented in this research are the result of 496 valid interviews. In all, there were 218 positive tests for COVID-19 and 278 negative ones. Among the positives, 44 during the circulation of the Delta variant and 176 during the circulation of the Omicrôn variant. The data analyzed and presented demonstrate how the universe of coronavirus infection is multifactorial and dependent on many conditions. This characteristic is even accentuated when the scenarios of two circulations of different variants are compared, as presented in the study. Some data are similar to what the pandemic presented at a global level (such as data related to gender), but others are well circumscribed to the Brazilian reality, or even regional, considering the region of residence where the participants lived (such as data related to race/color). Some behaviors associated with coronavirus control in national and international literature did not have similar results in this research, such as the number of vaccine doses, social isolation and working conditions. There was no significant variation between respondents who tested positive or negative. This situation can be explained by the profile of the respondents of this survey: they were symptomatic people who sought a diagnostic support service to find out whether or not they were contaminated by the coronavirus.
Desenvolvimento de detector de baixo custo para captação e análise de séries temporais de intervalos RR
(Universidade Brasil, 2024) Rizzato, Fernando Kendy Aoki.; Santos, Laurita dos
Digital health (e-Health) improves quality and life expectancy, with accessible devices like smartphones monitoring vital signs. In cardiology, due to the high mortality of cardiovascular diseases, the electrocardiogram (ECG) is crucial for assessing the heart's electrical activity and determining treatments. The prototype also applies linear and nonlinear analysis methods to characterize Heart Rate Variability (HRV), calculating indices such as RMSSD, standard deviation of heart rate, and geometric parameters of the Poincaré Plot. The system's validation involved comparing the measurements obtained by the prototype with a conventional ECG device in a population of individuals with different cardiovascular conditions. The results showed that for most time-domain and frequency-domain parameters, there were no statistically significant differences between the data collected by the prototype and the conventional ECG. However, parameters such as mean RR and mean HR showed significant differences. Linear regression analysis indicated a strong correlation between the HRV indices obtained by the prototype and the conventional method. The developed prototype proved to be an effective tool for capturing and analyzing RR intervals and HRV, providing results comparable to traditional methods and showing promise for clinical use in cardiovascular health assessment. To ensure the robustness of the data, adaptive analyses and statistical measures were conducted, utilizing Student's t-test, Mann-Whitney test, and linear regression, with a significance level of p < 0.05. This approach ensured that the conclusions drawn were based on solid and reliable evidence.
DEXA e Bioimpedância: comparação avaliativa em pacientes obesos na cidade de Cáceres, MT - Brasil
(Universidade Brasil, 2020) Dalbem, Cassiano Alfredo Garcia; Santos, Laurita dos
Obesity is a health problem known to increase the morbidity and mortality of individuals. Although widely used, BMI is not considered a good parameter to assess harmful levels of body fat, as it is not a good predictor of mortality. Several assessment methods have been proposed as a substitute for BMI over the years, with emphasis on the waist-to-hip ratio. Despite several articles evaluating it in a positive way, the predictive capacity of this method is still inconclusive. This study evaluates data on body components and correlates with central obesity and percentage of abdominal fat collected by BIA and DEXA equipment in a population in Cáceres - MT. The evaluated devices had similar performance, with statistical difference only between the measurements of the waist-hip ratio (p = 0.026). A non-parametric analysis of the data collected shows that waist circumference may be related to an increased risk of hypertension.
Diagnóstico clínico automatizado a partir do uso de métodos de análise multivariada aplicados a sinais de eletrocardiograma
(Universidade Brasil, 2020) Zena, Antonio Chavez; Mendes, Thiago de Oliveira; Santos, Laurita dos
This work presents a study of methods applied to pattern recognition of heart rate variability parameters obtained from electrocardiogram (ECG) for the aid of automated clinical diagnosis of various diseases associated with the heart, using multivariate statistical methods and computational machine learning. For this purpose, the electrocardiograms signs of 137 volunteers clinically diagnosed with normal sinus rhythm (NSR), with n = 54 individuals, which will represent the control group, and two clinical conditions formed by individuals with congestive heart failure (CHF), with n = 29 individuals or suppression of cardiac arrhythmia (CAST), with n = 54 individuals, considering these two clinical conditions, such as the case groups. All these signals were obtained from the PhysioNet, which covers a set of real biomedical signals, open source software and from studies consolidated in the literature. A procedure for obtaining characteristic variables of ECG tachograms was described, these variables were modeled by classification approaches of Discriminant Analysis data by Partial Least Squares regression (PLS-DA) and Artificial Neural Networks (ANN), aiming at the diagnosis of two clinical conditions when compared with a control group. Data matrices of variables associated with the time domain, frequency domain and obtained by non-linear methods were considered separately, each one, and all of these in a single data matrix, of statistical parameters associated with heart rate variability. The figures of merit showed that there is a pattern in the behavior of the tachogram parameters that may be used for clinical diagnostic aid. Both congestive heart failure and the classification and prediction of samples belonging to the cardiac arrhythmia suppression were satisfactorily obtained, with an area under the ROC curve close to 0.9. The PLS-DA model demonstrated the best data classification results, where congestive heart failure was diagnosed with rates of 90.9% of sensitivity and selectivity of 85.7% and suppression of cardiac arrhythmia was predicted with rates of 75.0% of sensitivity and 100.0 % of selectivity, suggesting that clinical diagnosis assisting real time and a personalized prognosis can become a reality that will contribute positively to medical practice.
Estudo da variáveis e variabilidade da frequência cardíaca nos nascidos a termo em um hospital público de Santarém - PA
(Universidade Brasil, 2022) Cardoso, Marina Chahini; Santos, Laurita dos
This study aimed to evaluate the findings of anthropometric variables and heart rate variability (HRV) in full-term newborns (NBs), correlating them with the socioeconomic factors of the parents of the NBs. Santarém, in the state of Pará. This is a descriptiveanalytical study, with a quantitative, transversal, documentary, comparative and prospective approach, using information collected from the medical records of 49 NBs, as well as the follow-up during the period of 4 months of these NBs. Data on the life habits of the parents of the participating NBs were obtained from a questionnaire applied to the mothers and the use of the Polar V800 in the NBs, to obtain information about HRV. The project was approved by the Research Ethics Committee of Universidade Brasil. The parents of the newborns in the study are adults between the ages of 20 and 29, brown, of normal weight, with a monthly income of one to two minimum wages. Most mothers had a slightly elevated BMI during pregnancy, with a tendency to normalize over the postpartum months. The dietary profile of parents is characterized by the consumption of proteins, carbohydrates, fruits and various vegetables. The evolution of anthropometric measurements: weight, length, BMI (body mass index) and HC (head circumference) of the newborns, followed what was considered normal by the WHO. The HRV metrics in the time domain, frequency domain and non-linear analyzed, showed a lower activity of the autonomic nervous system in the neonatal period in relation to the subsequent periods, within the study range, which can be attributed to the stressful events during the neonatal period. adaptation to the external environment. The HRV was used as a tool to determine a healthy evolution of the homeostatic system of NBs. Correlations of HRV variables with the parents' anthropometric and socioeconomic parameters did not show statistical significance. Even so, it was possible to demonstrate the evolutionary importance of the autonomic nervous system, relating it to linear and non-linear measures of HRV, with HRV being used as a marker of the adaptive physiological impact of NBs. As a benefit of the evaluation method, we must think that the knowledge of the HRV variables of a population of NBs should be used as a strategic marker in the promotion of health and quality of life of a population.
Influência do tempo de ozonólise no processo de ozonização de óleo vegetal utilizado para o reparo de pele
(Universidade Brasil, 2023) Tarocco, Juliana Carolina; Tim, Carla Roberta; Santos, Laurita dos
Lesões cutâneas, que são interrupções na continuidade das camadas da pele, afetam significativamente a qualidade de vida tanto de humanos quanto de animais. Essas lesões também implicam em altos custos para a rede de saúde pública e gastos consideráveis em clínicas e hospitais veterinários. Dado esse contexto, este estudo objetivou avaliar a influência do tempo de ozonólise durante o processo de ozonização do óleo de girassol com fluxo de O2 de 3L/min em uma concentração de O3 = 41,6 g/m3 no estímulo do reparo de feridas dérmicas em animais. Foram utilizados 35 ratos Wistar machos, que tiveram feridas induzidas por um punch de 10 mm. Estes foram distribuídos em grupos: controle (sem tratamento), e grupos tratados com óleo de girassol ozonizado por 30, 60, 90 e 120 minutos. Em cada lesão, foram administrados 80 uL do óleo ozonizado topicamente por 10 dias consecutivos. Na avaliação macroscópica, nenhum animal apresentou sinais de odor, necrose, fibrose ou presença de líquido na lesão. A análise do índice de reparo indicou que todos os grupos tiveram a mesma redução percentual da área lesionada. Similarmente, a análise histológica não mostrou diferenças no processo de reparo entre os grupos. A espectroscopia Raman diferenciou com sucesso a pele lesionada da pele saudável, mas não conseguiu distinguir entre o grupo controle e os grupos tratados com óleo ozonizado. Conclui-se que o tempo de ozonização do óleo, até 120 minutos, não alterou de forma significativa as estruturas químicas da pele nem estimulou seu reparo.
Laser Speckle Contrast Analysis (LASCA) aplicada à avaliação de estresse
(Universidade Brasil, 2024) Carvalho, Claudia Maria Sousa de; Amaral, Marcelo Magri; Santos, Laurita dos
Despite being physiologically normal, exaggerated and continuous exposure to stress, which exceeds the individual's tolerance capacity, can result in harm to health. The objective of this study was to apply the optical technique Laser Speckle Contrast Analysis (LASCA) to assess stress. This is a cross-sectional, descriptive, experimental study, with a quantitative approach, developed with undergraduate and postgraduate students, at a Higher Education Institution, in São Paulo (SP). The sample consisted of 27 (twenty-seven) participants, randomly distributed. The inclusion criteria were: undergraduate and postgraduate students, of both sexes, aged 18 or over, regularly enrolled. Those who self-reported having cardiovascular complications or using medication that altered cardiovascular functioning, suspected or confirmed pregnancy, physical limitations and women in their menstrual period were excluded. Data collection took place in June and August 2021 and all protective measures were adopted in relation to the coronavirus pandemic. Participants underwent cardiac signal acquisition to analyze heart rate variability, obtained using an electrocardiogram and a Polar V800 heart rate monitor, for 15 minutes. Simultaneously, the LASCA application was developed to obtain images using the MoorFLPI (Full-Field Laser Perfusion Imager) equipment. Next, participants underwent a physical effort test using an electric treadmill for 15 minutes, following the Bruce protocol. After resting for 5 minutes, cardiac signals and LASCA images were collected again, following the same protocols as in the initial phase. The project was approved by the Ethics and Research Committee of Universidade Brasil, under Opinion No. 4.724.843. Equivalent results were found regarding HRV measurements when comparing the LASCA technique and conventional methods, enabling the capture of signs of physiological changes present in the individual's state of stress. Thus, the results point to the feasibility of the LASCA technique to obtain images compatible with the heart rate pulsation pattern from the stress generated in situations of physical effort.
Modelo preditivo do nível glicêmico por monitoramento em tempo real em indivíduos portadores de diabetes mellitus tipo II
(Universidade Brasil, 2022) Mourão, Marcelo Henrique de Vasconcelos; Amaral, Marcelo Magri; Santos, Laurita dos
This research deals with a prediction of the glycemic levels of people with Diabetes Mellitus II, collected through a continuous glycemic monitoring system, based on the architecture of LSTM neural networks. Diabetes, one of the non-communicable chronic diseases, is characterized by hyperglycemia in the bloodstream generated by insulin resistance. The control of this disease can occur through carbohydrate counting according to the glycemic level, which according to the anthropometric evaluation is quantified by the physician. However, this approach is not always well accepted by diabetics, who end up adhering to medication for their control. Despite this, some diabetics end up using continuous blood glucose monitoring sensors, which favored verifying whether the glycemic data collected every 15 minutes could be predicted. The glycemia of 20 patients was measured over a period of 14 days using real-time monitoring. During this period, eating habits were recorded to count ingested carbohydrates, using the carbohydrate counting app created by SBD. Using an artificial intelligence model (LSTM) a blood glucose prediction model was created. With this model, it was verified that the predicted values followed the real glycemic movement, anticipating 5 hours with glycemic data of 12 continuous hours, that is, 20 predicted observations and 48 observations collected by the glycemia sensor for each individual. A general predictive model was performed with 20 volunteers and two personalized ones. The glycemic data of the collected diabetics had a positive performance, as the predicted values followed the glycemic movement, with a glycemic peak of 170 mg/dL at 9 am and 180 mg/dL at 1 pm, converging with the data obtained from the blood count. of carbohydrates, physical and anthropometric evaluation, observed with the peaks of glycemia, lifestyles of the volunteers and the total carbohydrates consumed daily. The glycemic data of non-diabetics had a positive performance, given that the predicted data followed the actual glycemic movement. This model, therefore, can predict several applications directly in rehabilitation, contributing as one of the important instruments for improving the patient's quality of life.
Ozonioterapia: regulamentação jurídica
(Universidade Brasil, 2020) Paez, Thalita Toffoli; Tim, Carla Roberta; Santos, Laurita dos
The present work aims to address the use of ozone therapy for treatment in humans, combined with the need to provide specific and complete legal guidelines. Ozone is a highly toxic gas, but it has an important antioxidant, bactericidal, anti-inflammatory characteristic and helps in the repair process. For this reason, the artificial production of this gas was started, for its therapeutic use, whose technique is called ozonotherapy. However, it is questioned whether the technique is a safe and effective treatment, since application in inadequate doses or routes of application can bring serious results to the patient. Currently, when the chosen route is auto-hemotherapy, it is possible that it is administered in a judicious and personal way, making the analysis of the individual's biomarkers and their tolerance to ozone, given the fact that the levels of gas tolerance are different in each subject. It turns out that, there is no specific legislation that ensures which professionals can prescribe and use ozone therapy, having several class councils already positioned themselves favorably. In 2018, the Ministry of Health incorporated ozone therapy as an integrative and complementary practice of the Unified Health System, provided it is applied in precise therapeutic doses, without, however, establishing criteria for the definition of the dose to be applied. Health is a social right that must be safeguarded in order to preserve a healthy and dignified life, which is why any health treatment can only be prescribed and carried out under appropriate conditions of safety and inspection, supported by scientific research and its own legislation. It is necessary to have specific and complete legislation on the subject, able to bring parameters and application protocols, in order to provide guarantees to professionals and patients who intend to treat. Thus, a research of the literature was carried out on electronic bases in the health and legal areas, there was no restriction on languages or publication data. Several articles were found, eliminating a duplication of titles and after reading abstracts, selected those that best fit the theme of this work. In a next step, perform a complete reading of all articles and the theoretical framework of this thesis was created. After reading and selecting the few and incomplete regulations on ozone therapy, he made a published work, which is a proposal for a complete review of the legislative content on ozone therapy. The proposed regulation brings the main parameters for the application of the technique, as well as the contraindicated routes, training of the professional who will manage the therapy, the qualified professionals, cautionary exams when due to auto-hemotherapy, care and responsibilities. In this sense, the present proposal of legislative content, was delivered to members of the Legislative Power, so that, observing the constitutional requirements, it can be used as a basis for the project, and eventually a future law, which will contain adequate and complete content regarding the theme.
Protocolo de exercício físico de curta duração na redução de riscos cardiovasculares em adolescentes
(Universidade Brasil, 2023) Batista, Mikael Henrique de Jesus; Santos, Laurita dos
The practice of physical activity in school is an important strategy to promote cardiovascular health in adolescents. Studies indicate that regular physical activity improves cardiorespiratory fitness and reduces cardiovascular risk factors in this population. Therefore, it is essential to encourage physical activity among adolescents, especially in school. Objective: To analyze the effect of a physical training protocol on cardiovascular risk factors among high school adolescents. Methods: This is a quantitative, experimental, prospective intervention study with an epidemiological approach. It was conducted at IFTO/Campus Colinas do Tocantins with 48 adolescents between 15 and 18 years old, divided into group 1, which did not participate in the intervention, and group 2, which underwent the intervention, consisting of 5 stages: 1st Socioclinical Data and Anthropometric Measurements Collection, 2nd Cardiac Signal Processing and Analysis, 3rd Blood Collection and Biochemical Analyses, 4th Léger Shuttle Run Test (20m), and 5th Intervention. Except for socioclinical data, all other stages were performed before and after the intervention. Results: A significant improvement was observed in group 2, which underwent the intervention with the application of a physical training protocol before extracurricular physical activity, presenting an improvement of 11.1% in adolescents with regular cardiorespiratory fitness compared to 6.7% in group 1, which participated in physical education classes. It should be noted that after physical education classes, it was possible to observe that the highest mean BMI and body fat were still associated with inadequate cardiorespiratory fitness (p=0.016, p=0.164), which did not occur in group 2, demonstrating positive effects of the intervention on the cardiorespiratory fitness of adolescents. In group 1, physical education classes showed an effect on the cardiovascular risk factor related to the percentage of body fat, with a reduction in the median of (-23.85mm) (p=<0.001). Conclusion: The intervention demonstrated efficacy in significantly improving cardiorespiratory fitness, and the moderate correlation between HRV and VO2max demonstrates the benefits of the intervention for improving autonomic nervous system adaptation and enhancing the cardiorespiratory capacity of this population.
Shampoo ozonizado: uma abordagem dermatocosmética no controle da dermatofitose
(Universidade Brasil, 2024) Peral, Alline Vasconcellos Alves; Tim, Carla Roberta; Santos, Laurita dos
Dermatophytosis are skin infections caused by dermatophytes, a group of keratinophilic fungi. Ozonated oil has better stability, making it easier to handle, improving storage, to avoid rapid degradation, allowing for out-of-hospital treatment. This therapeutic approach has been presented as an effective and low-cost alternative that should be considered for implementation in the public health system. The purpose of this research was to investigate the antifungal activity of ozonized shampoo against the Microsporum canis fungus that causes tinea capitis. Microsporum canis was used as a standard fungus strain. A direct contact test was carried out to evaluate the fungicidal effect of the ozonized shampoo. For this, the fungus M. canis was cultivated in Sabouraud Dextrose liquid medium and used at a concentration of 106/mL and added with ozonized shampoo, homogenized, and kept for 1 minute. Then, 50 µl were plated in glass Petri dishes in SDA culture medium (Sabouraud Dextrose Agar) and incubated for 15 days at 28°C. The fungicidal effect of the ozonized shampoo was also evaluated on human hair samples contaminated with M. canis, an ex vivo assay. In the laminar flow chamber, a volume of 50 μl, at a concentration of 106 CFU/mL of fungus was distributed in the center of each plate. Then, samples of natural human hair were placed on the plates to be contaminated with the fungus. The inoculated plates were incubated at 28°C for 14 days. It can be seen using light microscopy that the fungus M. canis has contaminated the hair, making it possible to see the presence of hyphae and conidia around the entire hair. In the direct contact test, the fungicidal effect of the ozonized shampoo was observed in the two concentrations evaluated for the fungus M. canis. After washing the hair samples, it was observed that the ozonized shampoo also had a fungicidal effect on hair contaminated with M. canis.
Uso de redes complexas ordinais para análise de variabilidade da frequência cardíaca
(Universidade Brasil, 2022) Costa, Gilberto de Araújo; Santos, Laurita dos; Azevedo, Francisco Honeidy Carvalho
Introduction: Efforts to apply the analysis of complex ordinal networks are being expanded and recognized as effective in the evaluation and interpretation of non-linear biological signals, including those obtained from electrocardiograms (ECGs) and electroencephalograms (EEGs). Ordinal symbolic analysis, that is, the analysis of ordinal representations, has some practical advantages. First of all, it is generally recognized as being conceptually simple and computationally fast. Furthermore, ordinal patterns, being defined by inequalities, are relatively robust against observational noise. For this reason, ordinal symbolic analysis has remained a popular method in biology and medicine, especially when it comes to distinguishing normal and abnormal health conditions in real time. Objective: To analyze the quantification measures of complex networks to apply them in the differentiation of time series of RR intervals of a group of healthy individuals and those with coronary artery disease. Material and Methods: This is a computational study that involves analysis of time series of RR intervals of healthy young people with coronary artery disease from a database. The time series was mapped for transformation into complex networks. From the complex networks, several parameters were obtained, such as entropy calculation, number of network edges, average degree of network, network density, and global clustering coefficient of the network. In the implementation of the mathematical analyses, Python and SPSS version 20.0 were used for the statistical analyses. In the first part of the work, the impact of dimension and delay parameters on entropy measurements was analyzed. In the second part, the total sample corresponding to 40time series of RR intervals, divided into two groups, were compared by applying the network quantification measures. Results and Discussion: The parameters had a directly proportional effect on entropy measurements up to a certain limit. Findings in the literature showed that the limitation in the analysis of ordinal networks is caused by the dissipation in the mapping process or by the lack of evidence of some types of signals. It was observed that to reduce the degenerations, the dimensions need to be large enough without, however, derail the computational analysis or excessively increasing the noise in the signals. In the quantifiers of the network, it was possible to evaluate the impact of the parameters on the efficacy, for the classification of healthy and coronary patients. Conclusion: Shannon entropy stood out in all dimensions for delay 6, showing better results in dimensions 5 and 6, with special emphasis on dimension 6 in several of them. Enabling the differentiation of time series of RR intervals of a group of healthy and coronary diseased individuals.