Shampoo ozonizado: uma abordagem dermatocosmética no controle da dermatofitose

Abstract

Dermatophytosis are skin infections caused by dermatophytes, a group of keratinophilic fungi. Ozonated oil has better stability, making it easier to handle, improving storage, to avoid rapid degradation, allowing for out-of-hospital treatment. This therapeutic approach has been presented as an effective and low-cost alternative that should be considered for implementation in the public health system. The purpose of this research was to investigate the antifungal activity of ozonized shampoo against the Microsporum canis fungus that causes tinea capitis. Microsporum canis was used as a standard fungus strain. A direct contact test was carried out to evaluate the fungicidal effect of the ozonized shampoo. For this, the fungus M. canis was cultivated in Sabouraud Dextrose liquid medium and used at a concentration of 106/mL and added with ozonized shampoo, homogenized, and kept for 1 minute. Then, 50 µl were plated in glass Petri dishes in SDA culture medium (Sabouraud Dextrose Agar) and incubated for 15 days at 28°C. The fungicidal effect of the ozonized shampoo was also evaluated on human hair samples contaminated with M. canis, an ex vivo assay. In the laminar flow chamber, a volume of 50 μl, at a concentration of 106 CFU/mL of fungus was distributed in the center of each plate. Then, samples of natural human hair were placed on the plates to be contaminated with the fungus. The inoculated plates were incubated at 28°C for 14 days. It can be seen using light microscopy that the fungus M. canis has contaminated the hair, making it possible to see the presence of hyphae and conidia around the entire hair. In the direct contact test, the fungicidal effect of the ozonized shampoo was observed in the two concentrations evaluated for the fungus M. canis. After washing the hair samples, it was observed that the ozonized shampoo also had a fungicidal effect on hair contaminated with M. canis.