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URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/914
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Resultados da Pesquisa
Item Caracterização de óleo de girassol ozonizado em diferentes tempos usando Espectroscopia de Infravermelho com Transformada de Fourier(Universidade Brasil, 2021) Uebele, Daniela; Santos, Laurita dos; Pessoa, Rodrigo SávioThe estimated number of patients in Brazil and abroad, emphasizes the need for better monitoring and treatment of skin lesions, caused by numerous health problems, in addition to worsening by viral infections, bacteria, fungi and protozoa - the prospect is of increasing cases in developing and underdeveloped countries. This is due to the inefficient treatments offered by hospitals, with an emphasis on public hospitals. These are more than enough reasons to invest time in the analysis of an affordable therapeutic treatment compared to the current ones - such as the use of "ozone therapy". This is recognized and used on 5 continents and is even encouraged and sponsored by the governments and health plans of many countries. One of the safest ways of using ozone in treatments is through the by-products generated in the ozonolysis of vegetable oils, which can act on the skin, leading to the disinfection process, by cell lysis. In this work, the choice was for sunflower oil due to its physicochemical characteristics. The objective is to analyze the chemical structure of ozonized sunflower oil at different times over 90 minutes, without using the temperature control of the ozonolysis reactor. For that, the process of analysis by Fourier transform spectroscopy (FTIR) was used. 9 spectra were collected and analyzed by the deconvolution process, in comparison to the main bands observed by the second derivative. The results show that there was an increase in oil temperature (around 10ºC) during ozonation up to 30min. Three regions of the obtained spectra were analyzed: 3050-2800cm-1 , 1130-1070cm-1 and 1800-800cm-1 . Statistical results show a significant difference between spectral signals, with observance after the time of 20min of the sunflower oil ozonation process, with emphasis on the 1800–1500cm 1 region, which demonstrates the rupture of the double carbon bonds (C=C), breaking the lipid chain, and increasing the CO2 chain and thus reducing HC=CH, observed in the analysis of the spectra. There was a reduction in the intensity of the -CH band of the stretching of the -C=CH fragment above the 3000cm-1 region, an increase in the CH stretching bands of the CH3 groups, a reduction in the intensity of the -C=C- binding stretch and constant intensity of the band stretch -C, and all changes detected by FTIR are in accordance with the Criegee mechanisms. With these observations it can be reiterated how useful FTIR spectroscopy is for the analysis of changes in compounds. Following the studies with the support of collaborators, initial tests of the application of the samples of the ozonized sunflower oil were carried out, extending up to 120 minutes, in cutaneous lesions in ex-vivo in an animal model, using the same analysis process, observing whether there were changes between the spectra. In the statistical analysis, these changes are subtle, not enough to obtain results. For this, it is necessary to analyze in a wider region of the spectrum. It was not possible to obtain evidence of its benefits in the healing process. In need of in-depth studies, considering other factors relevant to the study.Item Avaliação de diferentes concentrações de água ozonizada na desinfecção de canais radiculares contaminados: estudo in vitro(Universidade Brasil, 2022) Fernandes, Karina Gonzalez Camara; Garcia, Lívia Assis; Tim, Carla RobertaIn the clinical practice of dentistry, it is evident that despite technological advances, microbial agents are still the main responsible for endodontic failure. Mechanical chemical preparation, a technique used in endodontics, aims to remove necrotic tissue, microbial agents and their by-products from the root canal system. However, preparation is often not sufficient for satisfactory cleaning due to the anatomical complexity of the root canal system, which may lead to the permanence of microorganisms that may remain viable and active, interfering with the recovery process of periradicular tissues. In this way, it is necessary to search for ideal irrigating solutions that can provide better results in endodontic treatment. The aim of the present study was to evaluate and compare, in vitro, the effectiveness of different concentrations of ozonated water in the disinfection of root canals inoculated with Enterococcus Faecalis (E. Faecalis). We used 75 extracted human premolars, with a single canal, approved by the Research Ethics Committee (CEP), approval number 26629619.9.0000.5494. The teeth were sectioned and contaminated with E. Faecalis and incubated for 21 days at 37°C. Initial collections were performed with absorbent paper cones to confirm contamination. subsequently, PQM mechanical chemical preparation was performed, using mechanical instrumentation with a WaveOne® Primary file and the samples were divided into 5 groups according to the irrigating solutions (n = 15): Saline Solution Group (SS): irrigating solution was saline; 2.5% sodium hypochlorite group (NaOCl): 2.5% sodium hypochlorite irrigating solution; Ozone Group 10 (O10): the irrigating solution of ozonized water at a concentration of 10 µg/mL; Ozone Group 20 (O20): irrigating solution of ozonized water at a concentrationof 20 µg/mL; Ozone Group 30 (O30): irrigating solution of ozonized water at a concentration of 30 µg/mL. After mechanical instrumentation and irrigation, a second collection was performed with a paper cone, so the variables analyzed were the collections before and after the PQM. The main results show that the ozonated water used as an irrigating solution in the mechanical chemical preparation was effective in reducing the number of CFU Colony Forming Units at all concentrations (O10, 17,48%O20, 18,87% and O30, 32,87%) and the highest percentage of reduction was observed at the concentration of 30 μg/mL. However, it was not possible to observe a higher reduction of CFU in the groups treated with ozonated water when compared to the irrigant NaOCl. In conclusion, the ozonated water used as an irrigating solution, with emphasis on the concentration of 30 μg/mL, promoted antimicrobial action against E. Faecalis, one of the main microorganisms responsible for critical complications in endodontics, and can be proposed as an adjuvant in endodontic treatments.