Engenharia Biomédica
URI permanente para esta coleçãohttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/33
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Resultados da Pesquisa
Item Uso da Ozonioterapia em fungos causadores de Onicomicoses: estudo in vitro(Universidade Brasil, 2024) Del Castilo, Denise Vivianni Ferreira; Assis, Lívia; Tim, Carla Roberta: Onychomycosis is a common fungal infection of the nails, predominantly caused by dermatophytes such as Trichophyton rubrum (T. rubrum) and Microsporum gypseum (M. gypseum) and is a notoriously difficult condition to treat. Thus, the objective of the present study was to evaluate and compare, in vitro, the effectiveness of different times of ozone therapy on the main fungi that cause onychomycosis. Dermatophyte fungi T. rubrum (ATCC 28188) and M. gypseum (ATCC 24102) were incubated at 28° C for 14 days. Subsequently, the fungi were divided into 9 experimental groups according to the experimental time: Control Group (CG): the fungi were cultivated and did not receive any treatment; Cultivated fungi and received ozone therapy for a time of 2 min (G2”); 4 min (G4”); 6 min (G6”); 8 min (G8”); 10 min (G10”); 12 min (G12”); 14 min (G14”) and 16 min (G16”). The ozone concentration used was 2 µg/mL, oxygen flow of 1/4 L/min and applied dosages of 157, 314, 451, 628, 785, 943, 1100 and 1257 mg/m2, respectively. The main results show that the use of topical ozone therapy was effective in reducing the germination percentage of T. rubrum and M. gypseum in all experimental periods, presenting complete eradication with a treatment time of 6 min for T. rubrum and 14 min for M. gypseum. In conclusion, ozone therapy used topically, with emphasis on the treatment time of 6 min for T. rubrum (628 mg/m2) and 14 min (1257 mg/m2) for M. gypseum, promoted antifungal action on the main dermatophytes responsible for critical complications of onychomycosis, and may be proposed as a adjuvant in dermatological treatments.Item Influência do tempo de ozonólise no processo de ozonização de óleo vegetal utilizado para o reparo de pele(Universidade Brasil, 2023) Tarocco, Juliana Carolina; Tim, Carla Roberta; Santos, Laurita dosLesões cutâneas, que são interrupções na continuidade das camadas da pele, afetam significativamente a qualidade de vida tanto de humanos quanto de animais. Essas lesões também implicam em altos custos para a rede de saúde pública e gastos consideráveis em clínicas e hospitais veterinários. Dado esse contexto, este estudo objetivou avaliar a influência do tempo de ozonólise durante o processo de ozonização do óleo de girassol com fluxo de O2 de 3L/min em uma concentração de O3 = 41,6 g/m3 no estímulo do reparo de feridas dérmicas em animais. Foram utilizados 35 ratos Wistar machos, que tiveram feridas induzidas por um punch de 10 mm. Estes foram distribuídos em grupos: controle (sem tratamento), e grupos tratados com óleo de girassol ozonizado por 30, 60, 90 e 120 minutos. Em cada lesão, foram administrados 80 uL do óleo ozonizado topicamente por 10 dias consecutivos. Na avaliação macroscópica, nenhum animal apresentou sinais de odor, necrose, fibrose ou presença de líquido na lesão. A análise do índice de reparo indicou que todos os grupos tiveram a mesma redução percentual da área lesionada. Similarmente, a análise histológica não mostrou diferenças no processo de reparo entre os grupos. A espectroscopia Raman diferenciou com sucesso a pele lesionada da pele saudável, mas não conseguiu distinguir entre o grupo controle e os grupos tratados com óleo ozonizado. Conclui-se que o tempo de ozonização do óleo, até 120 minutos, não alterou de forma significativa as estruturas químicas da pele nem estimulou seu reparo.Item Uso de um gerador de ozônio de volume controlado na adiposidade localizada abdominal: ensaio clínico controlado e randomizado(Universidade Brasil, 2024) Silva, Fabiele Chieregato Marchetti da; Garcia, Lívia Assis; Tim, Carla RobertaOzone therapy has been suggested in clinical practice as a promising and adjuvant therapy in the minimally invasive treatment of localized adiposity; however, evidence is limited for its recommendations. Thus, the aim of the study was to evaluate the safety and efficacy of a controlled-volume ozone generator at different dosages in localized abdominal adiposity. To achieve this objective, a single-center randomized controlled clinical trial was conducted. Female participants were recruited and 110 were randomized into 4 groups: Sham Group (GS): participants received treatment with the ozone equipment turned off; Intervention Groups: participants received ozone treatment at doses of 20 (G20), 30 (G30), and 40 g/ml (G40). The interventions were performed at 24 pre-established points in the abdominal region, twice a week for a period of 4 weeks, totaling 8 sessions. The initial assessment (A1) and reassessment [15 (A2) and 30 (A3) days after the last session] were performed through anthropometric analyses, bioimpedance, abdominal circumference, adipometry, global aesthetics (GAIS), adipose tissue thickness through diagnostic ultrasound, recording of patient satisfaction assessment (Linkert) and adverse effects. The main results show that groups G20, G30 and G40 presented a reduction in abdominal circumference in the periods of 15 and 30 days after treatment, lower GAIS score and improvement in the treatment satisfaction score in both periods evaluated. However, G40 presented lower adipose tissue thickness in both experimental periods. No serious adverse effects were reported. Thus, it can be suggested that the controlled volume ozone generator used in the present study proved to be a safe and potentially promising modality in the treatment of localized adiposity, with emphasis on the dose of 40 ug/ml.