Monografias, Dissertações e Teses
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Resultados da Pesquisa
Item Efeito antimicrobiano de óleos essenciais sobre enterococcus faecalis(Universidade Brasil, 2024) Souto, Eliane Nogueira de Souza; Andreani Jr., Roberto; Navarrete, Acácio Aparecido; Fernandes, Karina CamaraIn the present research, the antimicrobial activity of essential oils against the standard strain of Enterococcus faecalis was evaluated. The minimum inhibitory concentration and the minimum bactericidal concentration of rosemary, lemongrass, oregano, and rosehip oils were determined. Additionally, the percentage reduction of colony-forming units in relation to different oil concentrations and exposure times was analyzed. With rosemary and oregano oils showing antibacterial activity at lower concentrations to inhibit bacterial growth (12.5% and 3.2%, respectively). It was found that these oils, along with lemongrass and rosehip oils, exhibited antibacterial activity against Enterococcus faecalis, both at a concentration of 25%. It was observed that the nullification of colony-forming unit counts was achieved in 120 minutes for rosemary oil at 12.5% and in 60 minutes for oregano oil, 180 minutes for lemongrass oil, and 210 minutes for rosehip oil. These results indicate the possibility of using essential oils in antibacterial therapy against infections caused by E. faecalis.Item Avaliação da eficácia de óleos vegetais in natura e ozonizados no controle de cândida spp.(Universidade Brasil, 2022) Gimenez, Vanessa Barbosa; Kozusny-Andreani, Dora InésCandida spp, is a pathogen associated with health, causes superficial or invasive infections with a high mortality rate. The pathogenicity is attributed to virulence factors such as adhesion, proteinases, phospholipases, and hemolysins. The emergence of strains resistant to conventional treatments, highlights the need to obtain highly effective drugs that do not generate microbial resistance, among which, ozonized vegetable oils have gained prominence for their wide range of antimicrobial effect, are effective in antifungal therapies, antibacterial and antiviral. In this context, the objective of this research was to evaluate the effectiveness of ozonated and in natura vegetable oils in the in vitro inactivation of Candida albicans ATCC 25923 and Candida tropicalis ATCC 4563. To analyze the antifungal activity, vegetable oils of coconut, palm oil, sunflower, olive and pequi, all fresh and ozonized in concentrations ranging from 0.32% to 100%. The minimum inhibitory concentration was determined by the plate microdilution method, and then the minimum fungicidal concentration and the fungicidal kinetics of the oils were verified. The results showed that the vegetable oils of sunflower, coconut, palm oil, pequi and olive, in natura and ozonized, inactivated Candida tropicalis and C. albicans, however the in natura oils of palm, sunflower and coconut presented minimum inhibitory and fungicidal concentrations higher than those of ozonized oils, while those of olive and pequi did not show differences. Regarding the ability to reduce colony-forming units as a function of time, it was found that olive and pequi oils, ozonated and in natura, were more effective. Based on the results, the vegetable oils indicate the possibility of using them in the therapy of Candida spp.Item Composição química e atividade antimicrobiana de óleos vegetais in natura e ozonizados em Acinetobacter baumannii(Universidade Brasil, 2021) Silva, Wagner Rafael da; Kozusny-Andreani, Dora InésThe study of infection control mechanisms caused by bacteria is a topic of interest to different professionals involved in the hospital environment. This work aimed to investigate the use of vegetable oils as an alternative treatment in the control of these infections. The bactericidal effect of canola, coconut and palm oil, in natura and ozonized oils, was evaluated under Acinetobacter baumannii, a species of bacteria that stands out as an important opportunistic pathogen and is responsible for infections related to the care of health. All the tested oil samples were characterized by the techniques of gas chromatography, I2 and peroxides index, the most altered characteristic under the effect of the ozonation process, passing from undetectable in the samples of in natura oils to the fractions 125,7 ± 0,2; 422,4 ± 0,2 and 434,8 ± 0,2 meq/Kg of Na2S2O3 for canola oil, coconut and ozonized palm oil. The reduction of microbial load in A. baumannii strains was 93,5, 71,6 and 85,9% for canola, coconut and in natura palm oils, respectively, and 96,2, 93,8 and 91,0% for the oils of canola, coconut and ozonized palm oil, respectively. In addition, the efficiency in the inhibition time of A. baumanni showed that ozonized oils present immediate bactericidal action, where their bacterial count in 10 min equates to the results of in natura oils after 30 min of incubation. These results revealed that the oils tested can be used in the control of bacterial infections and the ozonization of these oils potentiates the bactericidal action. The results showed, however, that the comparison between the microbial counts of in natura and ozonized oils did not point significant differences, since all the resulting p-values were higher than the level of significance adopted for the comparative test. A similar result was found in the comparison among ozonized oils in terms of microbial count, that is, there were no significant differences among microorganism counts when the different types of ozonized oils were compared.Item Efeito fungicida de óleos essenciais no controle da antracnose em pós-colheita de frutos de mamão = fungicidal effect of essential oils in controlling anthracnose in papaya fruit postharvest(Universidade Brasil, 2016) Barufi, Rafael Fontana; Kozusny-Andreani, Dora Inés; Andreani Junior, RobertoItem Eficácia de plantas medicinais no controle in vitro de Acinetobacter baumannii = medicinal plant efficiency in vitro control the Acinetobacter baumannii(Universidade Brasil, 2016) Andrade, Glaucimeire Rodrigues de; Kozusny-Andreani, Dora InêsItem Óleos essenciais no controle de Colletotrichum gloeosporioides = essential oils in control of Colletotrichum gloeosporioides(Universidade Camilo Castelo Branco, 2014) Ramos, Kelys; Kozusny-Andreani, Dora Inés; Andreani Junior, RobertoItem Efeito de óleos essenciais in natura e ozonizados sobre o desenvolvimento de fungos patogênicos(Universidade Brasil, 2020) Kume, Joelma Évelin Pereira; Kozusny-Andreani, Dora InésSome species of fungi are capable of causing mycotic infections. One of these infections is dermatophytosis, caused by a group of fungi, called dermatophytes. another infection is sporotrichosis, caused by species of Prothorax schenckii. The treatment of the disease is carried out using conventional antifungals. The emergence of resistant strains has led to alternative treatments, such as natural medicines or the use of ozone gas. The objective of this research was to evaluate the antifungal activity of fresh and ozonated essential oils against Trichophyton mentagrophytes, Trichophyton rubrum, and Sporothrix schenckii. In natura and ozonized essential oils of Cinnamomum cassia, Eugenia Caryphollata, Cymbopogon winterianus, Eucalyptus globulus, Eucalyptus staigeriana and Mentha piperita were used, evaluated for antifungal activity against the microorganism lineage. The oils were ozonized in corona equipment. The microdilution technique was used to assess the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC). The fungicidal kinetics of essential oils were determined. The data obtained were evaluated by the Mann-Whitney test and by the east of Kruskal-Wallis. The fungus T. mentagrophytes was more resistant. T. rubrum showed less resistance to treatments, showing decreases in microbial count in the first moments of exposure. The Spothrix fungus showed greater resistance to cinnamon, clove and Eucalyptus staigeriana oils, showing a decrease in microbial count variation. In general, the results show the possibility of use in antifungal therapy against the studied microorganisms.Item Eficácia de extratos de plantas medicinais no controle de micro-organismos isolados de ambientes de creche = Efficacy medicinal plants extracts in the control of isolate microorganisms from child care environments(Universidade Camilo Castelo Branco, 2013) Mendes, Elena Carla Batista; Kozusny-Andreani, Dora InêsItem Composição química e atividade antimicrobiana de óleos vegetais in natura e ozonizados em Acinetobacter baumannii(Universidade Brasil, 2021) Silva, Wagner Rafael da; Kozusny-Andreani, Dora InésThe study of infection control mechanisms caused by bacteria is a topic of interest to different professionals involved in the hospital environment. This work aimed to investigate the use of vegetable oils as an alternative treatment in the control of these infections. The bactericidal effect of canola, coconut and palm oil, in natura and ozonized oils, was evaluated under Acinetobacter baumannii, a species of bacteria that stands out as an important opportunistic pathogen and is responsible for infections related to the care of health. All the tested oil samples were characterized by the techniques of gas chromatography, I2 and peroxides index, the most altered characteristic under the effect of the ozonation process, passing from undetectable in the samples of in natura oils to the fractions 125,7 ± 0,2; 422,4 ± 0,2 and 434,8 ± 0,2 meq/Kg of Na2S2O3 for canola oil, coconut and ozonized palm oil. The reduction of microbial load in A. baumannii strains was 93,5, 71,6 and 85,9% for canola, coconut and in natura palm oils, respectively, and 96,2, 93,8 and 91,0% for the oils of canola, coconut and ozonized palm oil, respectively. In addition, the efficiency in the inhibition time of A. baumanni showed that ozonized oils present immediate bactericidal action, where their bacterial count in 10 min equates to the results of in natura oils after 30 min of incubation. These results revealed that the oils tested can be used in the control of bacterial infections and the ozonization of these oils potentiates the bactericidal action. The results showed, however, that the comparison between the microbial counts of in natura and ozonized oils did not point significant differences, since all the resulting p-values were higher than the level of significance adopted for the comparative test. A similar result was found in the comparison among ozonized oils in terms of microbial count, that is, there were no significant differences among microorganism counts when the different types of ozonized oils were compared.Item Avaliação in vitro de óleos essenciais de Copaíba, Buriti e Tucumã frente ao Sthapylococcus aureus(Universidade Brasil, 2017) Rezende, Aline Alves; Melo, Gabriel Maurício Peruca de; Bertipaglia, Liandra Maria Abaker