Ciências Ambientais
URI permanente para esta coleçãohttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/32
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Resultados da Pesquisa
Item Avaliação da eficácia de óleos vegetais in natura e ozonizados no controle de cândida spp.(Universidade Brasil, 2022) Gimenez, Vanessa Barbosa; Kozusny-Andreani, Dora InésCandida spp, is a pathogen associated with health, causes superficial or invasive infections with a high mortality rate. The pathogenicity is attributed to virulence factors such as adhesion, proteinases, phospholipases, and hemolysins. The emergence of strains resistant to conventional treatments, highlights the need to obtain highly effective drugs that do not generate microbial resistance, among which, ozonized vegetable oils have gained prominence for their wide range of antimicrobial effect, are effective in antifungal therapies, antibacterial and antiviral. In this context, the objective of this research was to evaluate the effectiveness of ozonated and in natura vegetable oils in the in vitro inactivation of Candida albicans ATCC 25923 and Candida tropicalis ATCC 4563. To analyze the antifungal activity, vegetable oils of coconut, palm oil, sunflower, olive and pequi, all fresh and ozonized in concentrations ranging from 0.32% to 100%. The minimum inhibitory concentration was determined by the plate microdilution method, and then the minimum fungicidal concentration and the fungicidal kinetics of the oils were verified. The results showed that the vegetable oils of sunflower, coconut, palm oil, pequi and olive, in natura and ozonized, inactivated Candida tropicalis and C. albicans, however the in natura oils of palm, sunflower and coconut presented minimum inhibitory and fungicidal concentrations higher than those of ozonized oils, while those of olive and pequi did not show differences. Regarding the ability to reduce colony-forming units as a function of time, it was found that olive and pequi oils, ozonated and in natura, were more effective. Based on the results, the vegetable oils indicate the possibility of using them in the therapy of Candida spp.Item Ozonização de Castanhas de Caju e do Brasil para o controle de fungos filamentosos(Universidade Brasil, 2020) Souza, Midian Nikel Alves de; Frias, Danila Fernanda RodriguesThe contamination of chestnuts by filamentous fungi, which produce mycotoxins, is considered a serious public health problem, which makes its control essential to obtain a quality and safe product for the consumer. Thus, the objective of this research was to evaluate the fungicidal effect of ozone against filamentous fungi isolated from cashew nuts and Brazil nuts. For this, 10 samples of chestnuts commercialized in bulk were used in the municipality of Fernandópolis – SP, from october to december, 2019. The search for filamentous fungi was carried out using the serial dilution method and plating on Potato Dextrose Agar. The dishes were incubated at 25ºC for 5 days, then the colony counts and genera identification were performed by observing the macroscopic characteristics and micromorphological structures. To test the effectiveness of ozone, 25g of chestnuts were diluted in 225 mL of 1% peptone water. This material was exposed to ozone at different times (5, 10, 15, 20, 25 and 30 minutes). In each time interval, 0,1 mL aliquots were inoculated in triplicate, in Petri dishes containing Potato Dextrose Agar, to determine the minimum fungicidal concentration (MFC). After tabulation of the collected data, two statistical analysis functions were performed: descriptive and inferential. The data were replicated in an absolute and relative way. In the inferential scope, the test Paired T Test was used. The fungal microbiota was detected in all samples analyzed, and in 70% of these, more than one genus was isolated. The genera were isolated: Aspergillus, Penicillium, Cladosporium, Curvularia, Absidia and Rhizopus. With the use of ozone, an average reduction of 96.5% of fungi can be seen in up to 30 minutes of exposure. It was concluded that ozonation was efficient in inactivating filamentous fungi and, consequently, in preventing mycotoxin synthesis.Item Efeito de óleos essenciais in natura e ozonizados sobre o desenvolvimento de fungos patogênicos(Universidade Brasil, 2020) Kume, Joelma Évelin Pereira; Kozusny-Andreani, Dora InésSome species of fungi are capable of causing mycotic infections. One of these infections is dermatophytosis, caused by a group of fungi, called dermatophytes. another infection is sporotrichosis, caused by species of Prothorax schenckii. The treatment of the disease is carried out using conventional antifungals. The emergence of resistant strains has led to alternative treatments, such as natural medicines or the use of ozone gas. The objective of this research was to evaluate the antifungal activity of fresh and ozonated essential oils against Trichophyton mentagrophytes, Trichophyton rubrum, and Sporothrix schenckii. In natura and ozonized essential oils of Cinnamomum cassia, Eugenia Caryphollata, Cymbopogon winterianus, Eucalyptus globulus, Eucalyptus staigeriana and Mentha piperita were used, evaluated for antifungal activity against the microorganism lineage. The oils were ozonized in corona equipment. The microdilution technique was used to assess the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC). The fungicidal kinetics of essential oils were determined. The data obtained were evaluated by the Mann-Whitney test and by the east of Kruskal-Wallis. The fungus T. mentagrophytes was more resistant. T. rubrum showed less resistance to treatments, showing decreases in microbial count in the first moments of exposure. The Spothrix fungus showed greater resistance to cinnamon, clove and Eucalyptus staigeriana oils, showing a decrease in microbial count variation. In general, the results show the possibility of use in antifungal therapy against the studied microorganisms.