Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Resultados da Pesquisa
Item Uso da Ozonioterapia em fungos causadores de Onicomicoses: estudo in vitro(Universidade Brasil, 2024) Del Castilo, Denise Vivianni Ferreira; Assis, Lívia; Tim, Carla Roberta: Onychomycosis is a common fungal infection of the nails, predominantly caused by dermatophytes such as Trichophyton rubrum (T. rubrum) and Microsporum gypseum (M. gypseum) and is a notoriously difficult condition to treat. Thus, the objective of the present study was to evaluate and compare, in vitro, the effectiveness of different times of ozone therapy on the main fungi that cause onychomycosis. Dermatophyte fungi T. rubrum (ATCC 28188) and M. gypseum (ATCC 24102) were incubated at 28° C for 14 days. Subsequently, the fungi were divided into 9 experimental groups according to the experimental time: Control Group (CG): the fungi were cultivated and did not receive any treatment; Cultivated fungi and received ozone therapy for a time of 2 min (G2”); 4 min (G4”); 6 min (G6”); 8 min (G8”); 10 min (G10”); 12 min (G12”); 14 min (G14”) and 16 min (G16”). The ozone concentration used was 2 µg/mL, oxygen flow of 1/4 L/min and applied dosages of 157, 314, 451, 628, 785, 943, 1100 and 1257 mg/m2, respectively. The main results show that the use of topical ozone therapy was effective in reducing the germination percentage of T. rubrum and M. gypseum in all experimental periods, presenting complete eradication with a treatment time of 6 min for T. rubrum and 14 min for M. gypseum. In conclusion, ozone therapy used topically, with emphasis on the treatment time of 6 min for T. rubrum (628 mg/m2) and 14 min (1257 mg/m2) for M. gypseum, promoted antifungal action on the main dermatophytes responsible for critical complications of onychomycosis, and may be proposed as a adjuvant in dermatological treatments.Item Caracterização de óleo de girassol ozonizado em diferentes tempos usando Espectroscopia de Infravermelho com Transformada de Fourier(Universidade Brasil, 2021) Uebele, Daniela; Santos, Laurita dos; Pessoa, Rodrigo SávioThe estimated number of patients in Brazil and abroad, emphasizes the need for better monitoring and treatment of skin lesions, caused by numerous health problems, in addition to worsening by viral infections, bacteria, fungi and protozoa - the prospect is of increasing cases in developing and underdeveloped countries. This is due to the inefficient treatments offered by hospitals, with an emphasis on public hospitals. These are more than enough reasons to invest time in the analysis of an affordable therapeutic treatment compared to the current ones - such as the use of "ozone therapy". This is recognized and used on 5 continents and is even encouraged and sponsored by the governments and health plans of many countries. One of the safest ways of using ozone in treatments is through the by-products generated in the ozonolysis of vegetable oils, which can act on the skin, leading to the disinfection process, by cell lysis. In this work, the choice was for sunflower oil due to its physicochemical characteristics. The objective is to analyze the chemical structure of ozonized sunflower oil at different times over 90 minutes, without using the temperature control of the ozonolysis reactor. For that, the process of analysis by Fourier transform spectroscopy (FTIR) was used. 9 spectra were collected and analyzed by the deconvolution process, in comparison to the main bands observed by the second derivative. The results show that there was an increase in oil temperature (around 10ºC) during ozonation up to 30min. Three regions of the obtained spectra were analyzed: 3050-2800cm-1 , 1130-1070cm-1 and 1800-800cm-1 . Statistical results show a significant difference between spectral signals, with observance after the time of 20min of the sunflower oil ozonation process, with emphasis on the 1800–1500cm 1 region, which demonstrates the rupture of the double carbon bonds (C=C), breaking the lipid chain, and increasing the CO2 chain and thus reducing HC=CH, observed in the analysis of the spectra. There was a reduction in the intensity of the -CH band of the stretching of the -C=CH fragment above the 3000cm-1 region, an increase in the CH stretching bands of the CH3 groups, a reduction in the intensity of the -C=C- binding stretch and constant intensity of the band stretch -C, and all changes detected by FTIR are in accordance with the Criegee mechanisms. With these observations it can be reiterated how useful FTIR spectroscopy is for the analysis of changes in compounds. Following the studies with the support of collaborators, initial tests of the application of the samples of the ozonized sunflower oil were carried out, extending up to 120 minutes, in cutaneous lesions in ex-vivo in an animal model, using the same analysis process, observing whether there were changes between the spectra. In the statistical analysis, these changes are subtle, not enough to obtain results. For this, it is necessary to analyze in a wider region of the spectrum. It was not possible to obtain evidence of its benefits in the healing process. In need of in-depth studies, considering other factors relevant to the study.Item Avaliação de diferentes concentrações de água ozonizada na desinfecção de canais radiculares contaminados: estudo in vitro(Universidade Brasil, 2022) Fernandes, Karina Gonzalez Camara; Garcia, Lívia Assis; Tim, Carla RobertaIn the clinical practice of dentistry, it is evident that despite technological advances, microbial agents are still the main responsible for endodontic failure. Mechanical chemical preparation, a technique used in endodontics, aims to remove necrotic tissue, microbial agents and their by-products from the root canal system. However, preparation is often not sufficient for satisfactory cleaning due to the anatomical complexity of the root canal system, which may lead to the permanence of microorganisms that may remain viable and active, interfering with the recovery process of periradicular tissues. In this way, it is necessary to search for ideal irrigating solutions that can provide better results in endodontic treatment. The aim of the present study was to evaluate and compare, in vitro, the effectiveness of different concentrations of ozonated water in the disinfection of root canals inoculated with Enterococcus Faecalis (E. Faecalis). We used 75 extracted human premolars, with a single canal, approved by the Research Ethics Committee (CEP), approval number 26629619.9.0000.5494. The teeth were sectioned and contaminated with E. Faecalis and incubated for 21 days at 37°C. Initial collections were performed with absorbent paper cones to confirm contamination. subsequently, PQM mechanical chemical preparation was performed, using mechanical instrumentation with a WaveOne® Primary file and the samples were divided into 5 groups according to the irrigating solutions (n = 15): Saline Solution Group (SS): irrigating solution was saline; 2.5% sodium hypochlorite group (NaOCl): 2.5% sodium hypochlorite irrigating solution; Ozone Group 10 (O10): the irrigating solution of ozonized water at a concentration of 10 µg/mL; Ozone Group 20 (O20): irrigating solution of ozonized water at a concentrationof 20 µg/mL; Ozone Group 30 (O30): irrigating solution of ozonized water at a concentration of 30 µg/mL. After mechanical instrumentation and irrigation, a second collection was performed with a paper cone, so the variables analyzed were the collections before and after the PQM. The main results show that the ozonated water used as an irrigating solution in the mechanical chemical preparation was effective in reducing the number of CFU Colony Forming Units at all concentrations (O10, 17,48%O20, 18,87% and O30, 32,87%) and the highest percentage of reduction was observed at the concentration of 30 μg/mL. However, it was not possible to observe a higher reduction of CFU in the groups treated with ozonated water when compared to the irrigant NaOCl. In conclusion, the ozonated water used as an irrigating solution, with emphasis on the concentration of 30 μg/mL, promoted antimicrobial action against E. Faecalis, one of the main microorganisms responsible for critical complications in endodontics, and can be proposed as an adjuvant in endodontic treatments.Item Ozonioterapia associada a um protocolo de exercício físico na dor e funcionalidade de indivíduos com osteoartrite de joelho: ensaio clínico controlado e randomizado(Universidade Brasil, 2022) Rezzo, Thiago Correia da Silva; Garcia, Lívia Assis; Tim, Carla RobertaKnee osteoarthritis (KOA) is a degenerative joint disease that causes pain, impairment of function and quality of life and is associated with significant economic impact. Ozone therapy (OT) has been suggested in clinical practice as a promising adjuvant therapy to exercise, however evidence is limited for its recommendation. Thus, the aim of this study was to evaluate the effects of OT in combination with an exercise protocol on pain level in women with KOA. Forty volunteers were randomized into 2 groups: Exercise Group (EG): volunteers were submitted to a physical exercise protocol; Ozone Therapy Exercise Group (GEO): volunteers were submitted to a resistance exercise protocol associated with OT. The interventions were performed twice a week for a period of 6 weeks, totaling 12 sessions. The exercise program consisted of warm up, muscle strengthening, and stretching of the main muscle groups of the lower limbs. OT was applied to 5 acupuncture points of the affected knee, through the subcutaneous technique at a concentration of 20 g/ml and with a volume of 2 mL of ozone immediately after each exercise session. For evaluation and reevaluation, the numerical pain scale (NDS) was applied; functionality and quality of life questionnaires (Western Ontario and MacMaster Universities Osteoarthritis Index [WOMAC]); functionality (Lequesne); physical performance tests (Timed Up and Go [TUG], Short Physical Performance Battery [SPPB]), lower limb strength and endurance (30-second sit and stand test). The results show that the GE and GEO groups presented lower scores for the level of pain, increased the score of quality of life, functionality, physical performance, strength and resistance compared to the initial values. However, GEO had a lower score for the level of pain, and a higher score for quality of life and functionality when compared to GE. Thus, it can be suggested that OT in association with physical exercise was an effective method in pain control and in the functional recovery and quality of life of patients with KOA.Item Ozonioterapia associada a um programa de exercício na dor e incapacidade de indivíduos com dor lombar crônica: ensaio clínico controlado e randomizado(Universidade Brasil, 2022) Silva, Rafael Basatos; Garcia, Lívia Assis; Tim, Carla RobertaChronic low back pain (CLBP) is one of the most common musculoskeletal disorders, prevalent worldwide and one of the main causes of disability and damage to quality of life, representing a significant economic burden. Ozone therapy (OT) has been suggested in clinical practice as an adjuvant therapy to physical exercise, however the evidence is limited for its recommendation. Thus, the aim of this study was to evaluate the effects of OT in combination with a physical exercise protocol on pain level in subjects with chronic low back pain. Forty-two volunteers were randomized into 2 groups: Control group (Ex): volunteers underwent a physical exercise protocol; Ozone Therapy Exercise Group (ExOT): volunteers underwent a resistance physical exercise protocol associated with OT. Interventions were performed twice a week for a period of 6 weeks, totaling 12 sessions. The exercise program consisted of exercises that strengthened, stretched, mobilized, coordinated and stabilized the abdominal, lumbar, pelvic and lower limb muscles. OT was applied at 5 points on the paravertebral muscles, bilaterally, totaling 10 points, through the intramuscular technique at a concentration of 20 g/ml and with a volume of 2 mL of ozone right after each proposed exercise session. For assessment and reassessment, the Numerical Pain Scale (END) was applied; disability index (Oswestry Disability Index); flexibility test (Third finger to floor test); trunk flexion/extension mobility test (Gonimetry); kinesiophobia (Kinesiophobia Cap Scale); emotional state (Anxiety Numerical Scale - ENA) and assessment of the perception of the global effect (Global Effect Perception Scale - EPEG). The results showed that both the Ex and the ExOT groups had a lower pain level, lower scores in disability tests, improved flexibility, lower scores in kinesiophobia when compared to the initial values. However, the lowest scores for the level of pain, disability and the highest scores for the level of perception of recovery were evidenced in the ExOT group. Thus, it can be suggested that OT associated with the physical exercise program was a more effective method in reducing pain and functional disability in the treatment of CLBP.Item Uso de um gerador de ozônio de volume controlado na adiposidade localizada abdominal: ensaio clínico controlado e randomizado(Universidade Brasil, 2024) Silva, Fabiele Chieregato Marchetti da; Garcia, Lívia Assis; Tim, Carla RobertaOzone therapy has been suggested in clinical practice as a promising and adjuvant therapy in the minimally invasive treatment of localized adiposity; however, evidence is limited for its recommendations. Thus, the aim of the study was to evaluate the safety and efficacy of a controlled-volume ozone generator at different dosages in localized abdominal adiposity. To achieve this objective, a single-center randomized controlled clinical trial was conducted. Female participants were recruited and 110 were randomized into 4 groups: Sham Group (GS): participants received treatment with the ozone equipment turned off; Intervention Groups: participants received ozone treatment at doses of 20 (G20), 30 (G30), and 40 g/ml (G40). The interventions were performed at 24 pre-established points in the abdominal region, twice a week for a period of 4 weeks, totaling 8 sessions. The initial assessment (A1) and reassessment [15 (A2) and 30 (A3) days after the last session] were performed through anthropometric analyses, bioimpedance, abdominal circumference, adipometry, global aesthetics (GAIS), adipose tissue thickness through diagnostic ultrasound, recording of patient satisfaction assessment (Linkert) and adverse effects. The main results show that groups G20, G30 and G40 presented a reduction in abdominal circumference in the periods of 15 and 30 days after treatment, lower GAIS score and improvement in the treatment satisfaction score in both periods evaluated. However, G40 presented lower adipose tissue thickness in both experimental periods. No serious adverse effects were reported. Thus, it can be suggested that the controlled volume ozone generator used in the present study proved to be a safe and potentially promising modality in the treatment of localized adiposity, with emphasis on the dose of 40 ug/ml.Item Aplicação subcutânea da Ozonioterapia no processo de reparação tecidual em modelo experimental de feridas cutâneas em ratos(Universidade Brasil, 2023) Muniz, Tânia Maria Quintal; Garcia, Lívia AssisItem Resposta endometrial após ozônio intrauterino em éguas(Universidade Brasil, 2017) Factor, Luana; Orlandi, Cássia Maria Barroso