Saúde
URI permanente para esta coleçãohttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/36
Navegar
3 resultados
Resultados da Pesquisa
Item Avaliação do efeito do extrato aquoso da casca do caule de “Ximenia Americana L.” incorporado à hidrogel à base de gelatina metacrilato (gelma) no processo de reparo ósseo induzido em ratos(Universidade Brasil, 2023) Leal, Seânia Santos; Costa, Adriana Pavinatto da; Oliveira, Rauirys Alencar deSeveral physiotherapeutic modalities have been studied with the aim of promoting bone repair. In the biomedical context, the use of Biomaterials has been studied to stimulate this process. The present work aims to produce, characterize and evaluate the effect of the stem bark extract of “Ximenia americana L.” incorporated into gelatin methacrylate hydrogel (GelMA) associated or not with photobiomodulation (LED) therapy in the bone repair process induced in rats. To this end, the materials GelMA and GelMA + stem bark extract of “Ximenia americana L.” that were used in in vivo experiments. Animal tests were carried out using 50 male Wistar rats distributed into 5 distinct groups called: control group (CG), GelMA group (GG), Gelma + LED group (GLED), GelMA group + Ximenia americana extract ( GE) and GelMA + Ximenia americana extract _ LED (GELED) group, which were subjected to the induced fracture protocol in the diaphyseal region of the right tibia and treated with light by emission diode 858 nm and energy dose 6J in an established period of 15 and 30 days, being euthanized throughout the same cycle. To verify the effects of the therapies, histological analyzes and Raman spectroscopy were carried out. The results showed that 15 days after inducing injury in the tibia of rats, in the GE group, bone neoformation was higher than in the control group and between the GG and GE groups. In 30 days of the experiment, there was also a difference in bone neoformation between the GELED group and the control group between the GE and control group. There was also a difference between the GG and GE and GELED groups. Given the results, it can be stated that the stem extract of “Ximenia americana L” incorporated into GelMA associated with photobiomodulation from LED is a potentiator of bone repair in an animal model.Item Efeitos do biopolímero de fibrina heterólogo e fotobiomodulação no reparo de feridas cutâneas em ratos(Universidade Brasil, 2021) Silva, Naldiana Cerqueira; Maia Filho, Antônio Luiz Martins; Tim, Carla RobertaCutaneous wounds are a public health problem in Brazil and worldwide with a negative impact on the lives of thousands of people, that raise treatment costs and make it difficult to maintain the sustainability of health systems. The aim of the present study was to evaluate the effect of LED-mediated-photobiomodulation therapy associated or not with the use of heterologous fibrin biopolymer (HFB) on the repair of cutaneous wounds in rats. Male Wistar rats were randomly divided into 4 groups of 21 animals as follows: control group (CG) whose wounds were treated with 0.9% saline solution; LED group (LED) whose wounds were irradiated with LED (660 nm, 166 mW, 60 s, 9.96 J); heterologous fibrin biopolymer group (HFB) whose wounds were treated exclusively with HFB; group LED + heterologous fibrin biopolymer (LED + HFB) whose wounds were irradiated with LED and HFB was applied. Two full-thickness cutaneous wounds were created on the back of each animal: a longitudinal wound measuring 3 cm and a circular wound with the diameter of 2 cm. The applications of LED and HFB were performed immediately after injury and followed an interval period of 72 hours between the posterior applications as described: with 2 applications for the 7-day groups, 4 applications for the 14-day groups and 6 applications for the 21-day groups. Animal euthanasia occurred in three different time periods: 7, 14 and 21 days after surgery. Macroscopic analysis, morphometric analysis of the wound repair index, descriptive histological and histomorphometric analysis, collagen quantification and tensile strength analysis. In the macroscopic findings, it was possible to observe the absence of necrosis, odor, presence of fibrosis or fluid in the lesion cavity in all experimental groups. Particularly In the control group, there was mild local inflammation evidenced between the fifth and sixth day after injury, that did not manifest in the other groups. The analysis of the wound repair index demonstrated that the treated groups presented greater wound reduction in the three periods of evaluation. Histopathological analysis revealed that the treatments presented a better repair process in comparison to the control group. Comparing the applied treatments, it was observed that only in the HFB group the dermis exhibited a denser extracellular matrix after 21 days. The collagen evaluation demonstrated that all treatments induced greater collagen deposition and maturation when compared to the control group. However, at 14 and 21 days the HFB group presents greater deposition of collagen type I and collagen maturation. The analysis of the traction force at 7 days of experiment showed better performance of the treated groups, while at 14 and 21 days the group that used HFB had greater resistance in the suture region. The results indicate that treatments using HFB and LED, either isolated or associated, stimulated the wound repair process in rats.Item Avaliação do óleo ozonizado e da fotobiomodulação no tratamento nos efeitos locais provocados pelo veneno da serpente Bothrops Jararacussu(Universidade Brasil, 2021) Fernandes, Jéssia Oliveira dos Santos; Navarro, Ricardo Scarparo; Cogo, José CarlosThe poisoning of the Bothrops jararacussu (jararacuçu) snake (B.jararacussu) causes local effects such as pain, swelling, inflammation, hemorrhage and myonecrosis. OBJECTIVE: The aim of this study was to evaluate the use of ozonized sunflower oil (OZO) associated or not with photobiomodulation (FBM) in the local effects promoted by Bothrops jararacussu venom. MATERIAL AND METHODS: Bothrops jararacussu venom was injected into the right gastrocnemius muscle (30 µg/50 µl NaCl 0.9%; i.m.) and the animals were euthanized 3 and 24 h later. The oil was ozonized using a corona generator (Ozone & Life® Model R&L1.5RM) and the FBM was used with the following parameters: Thera Laser, DMC®, red 685 nm, 4 J/cm2, 100 mW, 0.3 cm2, 1 .2 J, 12 s, spot. FBM was applied immediately after inoculation of the venom and OZO was also applied immediately after inoculation of the venom soaked in gauze for 15 minutes. In the control groups, the animals were injected with saline solution (NaCl 0.9%) and treated with OZO or FBM. In the groups injected with the venom, the animals were treated with OZO and FBM applied separately or together. The animals were anesthetized, the muscles removed, rinsed with PBS (phosphate buffer solution), fixed in 10% formaldehyde for 3 to 24 h, processed by routine histology techniques and stained with Hematoxylin and Eosin. The histological sections were analyzed under a light microscope and the cells counted and measured using the J imaging program. Necrotic and normal cells were evaluated, quantitative and morphometric analysis was performed, cellular edema was quantified by measuring the area and weight of the muscles and determined the levels of creatine kinase (CK) release. Data were submitted to ANOVA and Tukey-Kramer tests (p< 0.05). RESULTS: The results showed that the venom promotes myonecrosis of muscle cells characterized by edema and rupture of muscle cells. It promotes intracellular edema and CK release due to disruption of cell membranes. Treatment with FBM and OZO, applied separately, protects the muscle from the action of venom toxins, decreasing intracellular edema and decreasing CK release. OZO applied alone provided better protection than FBM. The association of FBM with OZO did not promote a synergistic effect in this protection. CONCLUSIONS: We conclude that due to the beneficial effects observed in these results, OZO can become an adjuvant treatment in poisoning. promoted by Bothrops jararacussu together with the antivenom, reducing the local harmful effects. This improvement is due to the therapeutic effects of OZO.