Avaliação do óleo ozonizado e da fotobiomodulação no tratamento nos efeitos locais provocados pelo veneno da serpente Bothrops Jararacussu
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Data
2021
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Universidade Brasil
Resumo
The poisoning of the Bothrops jararacussu (jararacuçu) snake (B.jararacussu) causes
local effects such as pain, swelling, inflammation, hemorrhage and myonecrosis.
OBJECTIVE: The aim of this study was to evaluate the use of ozonized sunflower oil
(OZO) associated or not with photobiomodulation (FBM) in the local effects promoted
by Bothrops jararacussu venom. MATERIAL AND METHODS: Bothrops jararacussu
venom was injected into the right gastrocnemius muscle (30 µg/50 µl NaCl 0.9%; i.m.)
and the animals were euthanized 3 and 24 h later. The oil was ozonized using a corona
generator (Ozone & Life® Model R&L1.5RM) and the FBM was used with the following
parameters: Thera Laser, DMC®, red 685 nm, 4 J/cm2, 100 mW, 0.3 cm2, 1 .2 J, 12
s, spot. FBM was applied immediately after inoculation of the venom and OZO was
also applied immediately after inoculation of the venom soaked in gauze for 15
minutes. In the control groups, the animals were injected with saline solution (NaCl
0.9%) and treated with OZO or FBM. In the groups injected with the venom, the animals
were treated with OZO and FBM applied separately or together. The animals were
anesthetized, the muscles removed, rinsed with PBS (phosphate buffer solution), fixed
in 10% formaldehyde for 3 to 24 h, processed by routine histology techniques and
stained with Hematoxylin and Eosin. The histological sections were analyzed under a
light microscope and the cells counted and measured using the J imaging program.
Necrotic and normal cells were evaluated, quantitative and morphometric analysis was
performed, cellular edema was quantified by measuring the area and weight of the
muscles and determined the levels of creatine kinase (CK) release. Data were
submitted to ANOVA and Tukey-Kramer tests (p< 0.05). RESULTS: The results
showed that the venom promotes myonecrosis of muscle cells characterized by edema
and rupture of muscle cells. It promotes intracellular edema and CK release due to
disruption of cell membranes. Treatment with FBM and OZO, applied separately,
protects the muscle from the action of venom toxins, decreasing intracellular edema
and decreasing CK release. OZO applied alone provided better protection than FBM.
The association of FBM with OZO did not promote a synergistic effect in this protection.
CONCLUSIONS: We conclude that due to the beneficial effects observed in these
results, OZO can become an adjuvant treatment in poisoning. promoted by Bothrops
jararacussu together with the antivenom, reducing the local harmful effects. This
improvement is due to the therapeutic effects of OZO.
Descrição
Palavras-chave
Veneno de Bothrops jararacussu, Fotobiomodulação, Óleo Ozonizado de Girassol