Avaliação in vitro dos efeitos de diferentes terapias antimicrobianas em canais uniradiculares infectados com Candida Albican

Abstract

Contamination of the root canal system is one of the main complications in endodontic treatment, where the necrotic pulp does not have blood vessels that can transport defense cells and antibiotics to the site. The aim of this study was to evaluate, in vitro, the fungal reduction of root canals treated with different antimicrobial therapies. Fifty-four bovine teeth were previously prepared for the manufacture of standardized single-root canals, with previous instrumentation with Hedströen files (Denstsplay®) #35, pre-enlarged with Gattes Gliden #4 drills (Denstsplay®) and instrumentation throughout its length with Reciproc R 50–25 mm files, using mechanized instrumentation with reciprocating movements. Then the root canals were contaminated with Candida albicans and submitted to different antimicrobial therapies: irrigation with 1% sodium hypochlorite for 5 min (GH); irrigation with 0.2% chlorhexidine for 5 min (GCHX); photodynamic therapy (aPDT antimicrobial Photodynamic Therapy), mediated by methylene blue (0.005%) and low power laser (Laser Duo, MMO, São Carlos, Brazil,