Avaliação microbiológica da associação do biopolímero de fibrina com nitrato de prata
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Data
2021
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Universidade Brasil
Resumo
Fibrin sealants, also known as fibrin biopolymer (BPF), are biological matrixes from
human plasma, fully resorbable, that mimic the last stages of the coagulation cascade
during which fibrinogen is converted into a structured and insoluble fibrin clot. BPF
developed from the snake venom Crotalus durissus terrificus, presents an excellent
alternative for tissue engineering and cell modulation. Its use as a healer for venous
ulcers in the lower limbs proved to be safe and potentially effective in a phase II clinical
study. Objective: To investigate the antimicrobial effect of the association of fibrin
biopolymer with silver nitrate. This association will allow its use in contaminated and
colonized wounds, which currently leads to suspension of treatment with BPF.
Methods: BPF is prepared from 2 active components and a diluent. Different
concentrations of silver nitrate were mixed with the BPF components. The clots
containing silver nitrate were inserted into the wells drilled on the agar in Petri dishes
containing the culture medium of Mueller Hilton agar previously seeded with cultures
of Staphylococcus aureus and Escherichia coli. Silver nitrate concentrations were
used, forming the following groups: Control - NP1 = 1mg / mL; NP2 = 2.5mg / mL and
NP3 = 5mg / mL silver nitrate; Experimental group: the same concentrations used in
the control group associated with BPF and positive control with antibiotics. The
presence was verified and the inhibition halo present in each of the tested plates was
measured. Tests of minimum inhibitory concentration (CIM) and minimum bactericide
(CBM) were carried out to establish the lowest concentration of BPF + NP and NP
capable of inhibiting the tested strains. Mean and median values of diameters with
measures of central tendency and standard deviation and interquartile range were
considered as measures of sample dispersion and verification of antimicrobial efficacy.
Results: A significant difference was observed in the halos of inhibition of treatments
with NP when compared with BPF + NP. There was a statistical difference in the
inhibition halos of the gram-negative bacteria in relation to the gram-positive bacteria,
in the treatment with NP, NP3 showed a significant difference in relation to NP2
compared to E. coli while S. aureus did not present a statistically significant difference.
BPF alone did not form an inhibition halo. BPF + NP had an inhibitory effect on the
antimicrobial action of NP by 18.2% for E. coli and 13.5% for S. aureus when compared
to NP treatment. The CIM found for E. coli and S. aureus when tested with GMP + NP
were 7.81 and 250 µg / mL and with NP 3.9 and 7.81 µg / mL. While CBM found for
BPF + NP they were 15.62 and 1000 µg / mL and for treatment with NP 7.81 and 15.62
µg / mL respectively. Conclusion: It was possible to verify by the results that the NP
and BPF + NP showed antibacterial effect on the species of gram-negative and grampositive bacteria, demonstrating greater efficacy for E. coli and that the antibacterial
activity of the NP was more effective than the BPF + NP. He observed that GMP when
isolated has no antibacterial effect.
Descrição
Palavras-chave
Biopolímero de fibrina, Nitrato de prata, Crotalus durissus terrificus, Antimicrobiano, Selante de fibrina heterólogo