Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Item Avaliação de hidrogel fibroso no processo de reparação de lesão cirúrgica em modelo diabéticos em ratos(Universidade Brasil, 2022) Calland, Franciléia Nogueira Albino; Lobo, Anderson de Oliveira; Oliveira, Francilio de CarvalhoIntroduction: Diabetes Mellitus is a cure for infections, as treatment is limited, treatment of this disease, consequences they are poor wound healing, as treatment is limited, treatment of this disease. GelMA is a non-toxic and biodegradable photopolymerizable hydrogel being a degraded hydrolyzed collagen, one of the most abundant components of the extracellular matrix (ECM), has been studied as an option in the treatment of wound healing. Objective: To produce a methacrylate base for diabetic foot skin modified with a methacrylate gelatin base for diabetic foot tissue skin. Materials and methods: Four types of hydrogels were tested in rats, previously defined in 5 groups of hydrogels, were defined in 5 control groups, G10% group, P1 group, PCM group, PGel group. Fibroblast counting was performed on slides stained with the HE method, from the injured areas on the 7th and 14th day, on the stained slides the number of fibroblasts was counted. The collection of Picrosirus Red (PSR) was used todetect the presence or not, as a scanning electron microscopy was used to provide information about the size, shape and arrangement of the particles. Results and discussion: Among the hydrogels tested on the 7th day of the protocol, the P12% and PCM groups obtained greater statistical significance in the number of fibroblasts found, compared to PGel and G10%, on the 14th day the hydrogel had a greater number of fibroblasts found for the PGel group. Fibroblasts are the main cells that do not present any healing process, so it is believed that dressings based on these cells can help in the treatment of wounds in patientswith DM. Conclusion: The group that performed better for PCL-modified/GEL based on the results presented the amount of fibroblasts, as well as the amount of collagen type III and type I, in this way, suggesting a beneficial effect in thematerial change for applications.Item Desenvolvimento de um Hidrogel à base de Laponita com liberação controlada de oxigênio para aplicações em feridas cutâneas infectadas(Universidade Brasil, 2020) Costa Neto, Almiro Mendes da; Marciano, Fernanda Roberta; Lobo, Anderson de OliveiraSkin wounds are a worldwide problem with significant morbidity and mortality, particularly when infection occurs. The most common pathogens are Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In tissue repair, control of bacterial colonization is essential. Biomaterials are used in this process, among them, hydrogels with nanoparticles. This work aims at the production of hydrogel based on laponite with controlled release of oxygen for application in infected skin wounds. Many clays are added to polymers to improve their properties, among them, Laponite of formula Na + 0.7 [(Si8Mg5.5Li0.3) O20 (OH) 4]. Substances have been associated with hydrogels to be released into wounds, including oxides in aqueous media. This is an experimental, descriptive and quantitative study verifying in vitro the bactericidal activity of laponite hydrogel with calcium peroxide (CPO) releasing oxygen. E. Coli and S. Aureus were grown. They were formulated in 9% laponite hydrogels with 5% CPO, 10% and 20% laponite. The bactericidal activity of the hydrogels was verified by the size of the inhibitory halo, in millimeters. The relationship between the size of the inhibitory halos and the concentrations was verified through Pearson's Correlation with a significance level of 5%. During the first 5h, the CPO hydrogels in laponite showed increased oxygen release. Considering the release of oxygen present in the hydrogel, dispersing through the medium and inhibiting microbial growth, it was possible to observe the formation of inhibition halos in all tested concentrations. The results showed that the three formulations of 9% laponite containing calcium peroxide were able to inhibit microbial growth by 100%, with halos greater than 9 mm against both bacterial strains. Against S. aureus, it was found that the correlation between the size of the inhibitory halos and the activity of the hydrogels at different concentrations showed a statistically significant difference (p <0.05). Against E. coli there is a strong correlation between the size of the inhibitory halos and the highest concentrations of hydrogel, particularly at 10%, which showed a statistically significant difference. Laponite acted as an efficient barrier to oxygen and showed antomicrobial activities and the hydrogel produced was a potential candidate for the treatment of infections and healing of skin wounds.