Avaliação de hidrogel fibroso no processo de reparação de lesão cirúrgica em modelo diabéticos em ratos

Abstract

Introduction: Diabetes Mellitus is a cure for infections, as treatment is limited, treatment of this disease, consequences they are poor wound healing, as treatment is limited, treatment of this disease. GelMA is a non-toxic and biodegradable photopolymerizable hydrogel being a degraded hydrolyzed collagen, one of the most abundant components of the extracellular matrix (ECM), has been studied as an option in the treatment of wound healing. Objective: To produce a methacrylate base for diabetic foot skin modified with a methacrylate gelatin base for diabetic foot tissue skin. Materials and methods: Four types of hydrogels were tested in rats, previously defined in 5 groups of hydrogels, were defined in 5 control groups, G10% group, P1 group, PCM group, PGel group. Fibroblast counting was performed on slides stained with the HE method, from the injured areas on the 7th and 14th day, on the stained slides the number of fibroblasts was counted. The collection of Picrosirus Red (PSR) was used todetect the presence or not, as a scanning electron microscopy was used to provide information about the size, shape and arrangement of the particles. Results and discussion: Among the hydrogels tested on the 7th day of the protocol, the P12% and PCM groups obtained greater statistical significance in the number of fibroblasts found, compared to PGel and G10%, on the 14th day the hydrogel had a greater number of fibroblasts found for the PGel group. Fibroblasts are the main cells that do not present any healing process, so it is believed that dressings based on these cells can help in the treatment of wounds in patientswith DM. Conclusion: The group that performed better for PCL-modified/GEL based on the results presented the amount of fibroblasts, as well as the amount of collagen type III and type I, in this way, suggesting a beneficial effect in thematerial change for applications.