Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Item Avaliação de hidrogel fibroso no processo de reparação de lesão cirúrgica em modelo diabéticos em ratos(Universidade Brasil, 2022) Calland, Franciléia Nogueira Albino; Lobo, Anderson de Oliveira; Oliveira, Francilio de CarvalhoIntroduction: Diabetes Mellitus is a cure for infections, as treatment is limited, treatment of this disease, consequences they are poor wound healing, as treatment is limited, treatment of this disease. GelMA is a non-toxic and biodegradable photopolymerizable hydrogel being a degraded hydrolyzed collagen, one of the most abundant components of the extracellular matrix (ECM), has been studied as an option in the treatment of wound healing. Objective: To produce a methacrylate base for diabetic foot skin modified with a methacrylate gelatin base for diabetic foot tissue skin. Materials and methods: Four types of hydrogels were tested in rats, previously defined in 5 groups of hydrogels, were defined in 5 control groups, G10% group, P1 group, PCM group, PGel group. Fibroblast counting was performed on slides stained with the HE method, from the injured areas on the 7th and 14th day, on the stained slides the number of fibroblasts was counted. The collection of Picrosirus Red (PSR) was used todetect the presence or not, as a scanning electron microscopy was used to provide information about the size, shape and arrangement of the particles. Results and discussion: Among the hydrogels tested on the 7th day of the protocol, the P12% and PCM groups obtained greater statistical significance in the number of fibroblasts found, compared to PGel and G10%, on the 14th day the hydrogel had a greater number of fibroblasts found for the PGel group. Fibroblasts are the main cells that do not present any healing process, so it is believed that dressings based on these cells can help in the treatment of wounds in patientswith DM. Conclusion: The group that performed better for PCL-modified/GEL based on the results presented the amount of fibroblasts, as well as the amount of collagen type III and type I, in this way, suggesting a beneficial effect in thematerial change for applications.Item Membrana à base de Quitosana com diferentes massas molares: produção, caracterização e bioatividade(Universidade Brasil, 2023) Macedo, Juliana Bezerra; Pavinatto, AdrianaChitosan is a polysaccharide widely used in biomedical applications due to its bioactivity, which is often related and can be modulated by its structural characteristics. Among these characteristics, molar mass is one that influences its action and, as cited in the literature, it may have different activity depending on the microorganism studied. In this context, this study aimed to evaluate the bioactivity of four types of membranes formed by pure high (QAMM) and low (QBMM) molar mass chitosans associated with turmeric (QAMM + turmeric and QBMM + turmeric), produced using the technique casting thin film formation (solvent evaporation). For physicochemical and morphological characterization of the membranes, thickness measurement techniques, absorption spectroscopy in the infrared region (FTIR) and contact angle were used. To carry out the microbiological tests, strains of the fungus Trychophyton rubrum, the gram-positive bacterium Staphylococcus aureus and the gram-negative bacterium Escherichia coli were used, and the bioactivity of the membranes was evaluated through the inhibition halo formed. To evaluate cell viability/cytotoxicity, fibroblast cell lines (L929) were used at a concentration of 1x104 using the alamarBlue® and Picrogreen assay. The methodology used to manufacture membranes was successful, making it possible to obtain self-supporting and homogeneous membranes, with thicknesses ranging from 35 to 56 μm. FTIR spectra show the main vibrational bands for chemical groups in chitosan. Contact angle results show that membranes containing turmeric are more hydrophilic than pure chitosan. The membranes did not show antimicrobial growth inhibition properties against the tested microorganisms. However, the presence of an inhibition halo was verified under bacterial cultures in tests using the membrane preparation solutions, with the QBMM and QBMM + turmeric solutions showing a greater inhibitory effect. The membranes produced did not present a cytotoxic effect, as they induced an increase in cell proliferation. In general, QBMM+ turmeric provided greater cell proliferation compared to that observed using other membranes. The results of the present study suggest that the membranes produced are promising materials for application in tissue engineering, favoring their manufacturing and optimal use.