Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Item Membranas de quitosana associada a cúrcuma: produção, caracterização e avaliação da biocompatibilidade e efeitos antimicrobianos(Universidade Brasil, 2024) Macedo, Daniela Bezerra; Assis, Lívia; Pavinatto, AdrianaSkin wounds cause pain, functional impairment and quality of life and are associated with significant economic impact. Chitosan and turmeric have properties that have the potential to promote the repair of skin wounds. However, the relationship between medium molar mass chitosan associated or not with turmeric, as well as the physicochemical properties and biological effects have not yet been elucidated. Thus, the objective of the present study was to produce, characterize and evaluate, in vitro, the cytotoxicity of a membrane based on medium molar mass chitosan associated or not with turmeric in a fibroblast line (L929), as well as the antimicrobial potential for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Three chitosan based membranes of medium molar mass were produced using the casting (solvent evaporation) thin film formation technique: 1% chitosan-based membrane (m/v; QM); chitosan associated with 30% glycerol (m/m in relation to the mass of chitosan; QMG); chitosan associated with 30% glycerol and 1.5% turmeric (m/m in relation to the mass of chitosan; QMGC). To characterize the physicochemical and morphological properties of the membranes, infrared absorption spectroscopy (FTIR), contact angle and thickness measurements were used. To evaluate cell viability/cytotoxicity, L929 cells were used at a concentration of 1x104 using the alamarBlue® and Picrogreen assay; To evaluate the antimicrobial potential, strains of Gram-positive bacteria S. aureus and Gram-negative bacteria E. coli were used at a concentration of 1 x 108 by evaluating the inhibition halo. The results show that all membranes were sustainable, homogeneous and flexible, with the QMG and QMGC membranes being the most flexible and most hydrophilic compared to the QM membrane. Vibrational bands were observed for chemical groups of chitosan and glycerol in all membranes. Regarding the evaluation of cytotoxicity, all membranes showed higher values of metabolic activity/viability, as well as a greater proliferation of fibroblast cells in the experimental period of 72 hours when compared to 24 hours of culture. Furthermore, all membranes presented an inhibition halo to the tested microorganisms, with the QMGC membrane promoting the greatest inhibition halo. With the results obtained in the present study, it is possible to confirm the reparative potential of chitosan-based membranes of medium molar mass, as well as their antimicrobial potential, with emphasis on the membrane associated with turmeric. However, further studies are needed to elucidate the use of these membranes as potential biomaterials for bioactive wound dressings.Item Produção e utilização de membranas à base de quitosana e cúrcuma no processo de reparação tecidual(Universidade Brasil, 2022) Pacheco, Karoline Maria Lopes; Costa, Adriana Pavinatto daTissue repair is a complex and dynamic pathophysiological process that involves distinct overlapping phases. In this context, biopolymers and natural extracts have been widely used in the development of new materials that favor the repair process. Chitosan and turmeric appear as an interesting alternative, as they have repair and antimicrobial properties, respectively. In the present work, membranes based on chitosan and turmeric were produced, characterized, and used for in vivo experiments with potential application for skin repair. The membranes were produced through the casting technique (evaporation of the solvent) of film formation, being produced membranes of chitosan 1% (m/v), chitosan mixed with glycerol 30% (m/m in relation to the mass of chitosan) and chitosan mixed with 30% glycerol + 1.5% turmeric (m/m in relation to chitosan mass). The characterizations of the physicochemical and mechanical properties of the membranes were made through measurements of absorption spectroscopy in the infrared region (FTIR), contact angle, thickness measurements and mechanical tests. Sustainable, homogeneous, and flexible membranes were obtained from all materials tested. The FTIR spectra show the main vibrational bands for chitosan and glycerol chemical groups, no band referring to turmeric was seen. Mechanical tests have shown that membranes containing glycerol are more flexible than those formed with pure chitosan. Membranes formed with glycerol and glycerol + turmeric are more hydrophilic compared to the membrane formed by pure chitosan. The results of the in vivo experiments show that the group that received the membrane of chitosan/glycerol 30%/turmeric 1.5% showed a statistically greater reduction in the injured area, as well as better results in the histological analysis, compared to the other experimental groups. The material developed here, from a natural source, low cost and easy to apply, capable of accelerating the process of repairing skin lesions, constitutes a contribution in the current literature.