Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Resultados da Pesquisa
Item Composição química e atividade antimicrobiana de óleos vegetais in natura e ozonizados em Acinetobacter baumannii(Universidade Brasil, 2021) Silva, Wagner Rafael da; Kozusny-Andreani, Dora InésThe study of infection control mechanisms caused by bacteria is a topic of interest to different professionals involved in the hospital environment. This work aimed to investigate the use of vegetable oils as an alternative treatment in the control of these infections. The bactericidal effect of canola, coconut and palm oil, in natura and ozonized oils, was evaluated under Acinetobacter baumannii, a species of bacteria that stands out as an important opportunistic pathogen and is responsible for infections related to the care of health. All the tested oil samples were characterized by the techniques of gas chromatography, I2 and peroxides index, the most altered characteristic under the effect of the ozonation process, passing from undetectable in the samples of in natura oils to the fractions 125,7 ± 0,2; 422,4 ± 0,2 and 434,8 ± 0,2 meq/Kg of Na2S2O3 for canola oil, coconut and ozonized palm oil. The reduction of microbial load in A. baumannii strains was 93,5, 71,6 and 85,9% for canola, coconut and in natura palm oils, respectively, and 96,2, 93,8 and 91,0% for the oils of canola, coconut and ozonized palm oil, respectively. In addition, the efficiency in the inhibition time of A. baumanni showed that ozonized oils present immediate bactericidal action, where their bacterial count in 10 min equates to the results of in natura oils after 30 min of incubation. These results revealed that the oils tested can be used in the control of bacterial infections and the ozonization of these oils potentiates the bactericidal action. The results showed, however, that the comparison between the microbial counts of in natura and ozonized oils did not point significant differences, since all the resulting p-values were higher than the level of significance adopted for the comparative test. A similar result was found in the comparison among ozonized oils in terms of microbial count, that is, there were no significant differences among microorganism counts when the different types of ozonized oils were compared.Item Desenvolvimento de um Hidrogel à base de Laponita com liberação controlada de oxigênio para aplicações em feridas cutâneas infectadas(Universidade Brasil, 2020) Costa Neto, Almiro Mendes da; Marciano, Fernanda Roberta; Lobo, Anderson de OliveiraSkin wounds are a worldwide problem with significant morbidity and mortality, particularly when infection occurs. The most common pathogens are Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. In tissue repair, control of bacterial colonization is essential. Biomaterials are used in this process, among them, hydrogels with nanoparticles. This work aims at the production of hydrogel based on laponite with controlled release of oxygen for application in infected skin wounds. Many clays are added to polymers to improve their properties, among them, Laponite of formula Na + 0.7 [(Si8Mg5.5Li0.3) O20 (OH) 4]. Substances have been associated with hydrogels to be released into wounds, including oxides in aqueous media. This is an experimental, descriptive and quantitative study verifying in vitro the bactericidal activity of laponite hydrogel with calcium peroxide (CPO) releasing oxygen. E. Coli and S. Aureus were grown. They were formulated in 9% laponite hydrogels with 5% CPO, 10% and 20% laponite. The bactericidal activity of the hydrogels was verified by the size of the inhibitory halo, in millimeters. The relationship between the size of the inhibitory halos and the concentrations was verified through Pearson's Correlation with a significance level of 5%. During the first 5h, the CPO hydrogels in laponite showed increased oxygen release. Considering the release of oxygen present in the hydrogel, dispersing through the medium and inhibiting microbial growth, it was possible to observe the formation of inhibition halos in all tested concentrations. The results showed that the three formulations of 9% laponite containing calcium peroxide were able to inhibit microbial growth by 100%, with halos greater than 9 mm against both bacterial strains. Against S. aureus, it was found that the correlation between the size of the inhibitory halos and the activity of the hydrogels at different concentrations showed a statistically significant difference (p <0.05). Against E. coli there is a strong correlation between the size of the inhibitory halos and the highest concentrations of hydrogel, particularly at 10%, which showed a statistically significant difference. Laponite acted as an efficient barrier to oxygen and showed antomicrobial activities and the hydrogel produced was a potential candidate for the treatment of infections and healing of skin wounds.Item Composição química e atividade antimicrobiana de óleos vegetais in natura e ozonizados em Acinetobacter baumannii(Universidade Brasil, 2021) Silva, Wagner Rafael da; Kozusny-Andreani, Dora InésThe study of infection control mechanisms caused by bacteria is a topic of interest to different professionals involved in the hospital environment. This work aimed to investigate the use of vegetable oils as an alternative treatment in the control of these infections. The bactericidal effect of canola, coconut and palm oil, in natura and ozonized oils, was evaluated under Acinetobacter baumannii, a species of bacteria that stands out as an important opportunistic pathogen and is responsible for infections related to the care of health. All the tested oil samples were characterized by the techniques of gas chromatography, I2 and peroxides index, the most altered characteristic under the effect of the ozonation process, passing from undetectable in the samples of in natura oils to the fractions 125,7 ± 0,2; 422,4 ± 0,2 and 434,8 ± 0,2 meq/Kg of Na2S2O3 for canola oil, coconut and ozonized palm oil. The reduction of microbial load in A. baumannii strains was 93,5, 71,6 and 85,9% for canola, coconut and in natura palm oils, respectively, and 96,2, 93,8 and 91,0% for the oils of canola, coconut and ozonized palm oil, respectively. In addition, the efficiency in the inhibition time of A. baumanni showed that ozonized oils present immediate bactericidal action, where their bacterial count in 10 min equates to the results of in natura oils after 30 min of incubation. These results revealed that the oils tested can be used in the control of bacterial infections and the ozonization of these oils potentiates the bactericidal action. The results showed, however, that the comparison between the microbial counts of in natura and ozonized oils did not point significant differences, since all the resulting p-values were higher than the level of significance adopted for the comparative test. A similar result was found in the comparison among ozonized oils in terms of microbial count, that is, there were no significant differences among microorganism counts when the different types of ozonized oils were compared.