Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Resultados da Pesquisa
Item Membrana à base de Quitosana com diferentes massas molares: produção, caracterização e bioatividade(Universidade Brasil, 2023) Macedo, Juliana Bezerra; Pavinatto, AdrianaChitosan is a polysaccharide widely used in biomedical applications due to its bioactivity, which is often related and can be modulated by its structural characteristics. Among these characteristics, molar mass is one that influences its action and, as cited in the literature, it may have different activity depending on the microorganism studied. In this context, this study aimed to evaluate the bioactivity of four types of membranes formed by pure high (QAMM) and low (QBMM) molar mass chitosans associated with turmeric (QAMM + turmeric and QBMM + turmeric), produced using the technique casting thin film formation (solvent evaporation). For physicochemical and morphological characterization of the membranes, thickness measurement techniques, absorption spectroscopy in the infrared region (FTIR) and contact angle were used. To carry out the microbiological tests, strains of the fungus Trychophyton rubrum, the gram-positive bacterium Staphylococcus aureus and the gram-negative bacterium Escherichia coli were used, and the bioactivity of the membranes was evaluated through the inhibition halo formed. To evaluate cell viability/cytotoxicity, fibroblast cell lines (L929) were used at a concentration of 1x104 using the alamarBlue® and Picrogreen assay. The methodology used to manufacture membranes was successful, making it possible to obtain self-supporting and homogeneous membranes, with thicknesses ranging from 35 to 56 μm. FTIR spectra show the main vibrational bands for chemical groups in chitosan. Contact angle results show that membranes containing turmeric are more hydrophilic than pure chitosan. The membranes did not show antimicrobial growth inhibition properties against the tested microorganisms. However, the presence of an inhibition halo was verified under bacterial cultures in tests using the membrane preparation solutions, with the QBMM and QBMM + turmeric solutions showing a greater inhibitory effect. The membranes produced did not present a cytotoxic effect, as they induced an increase in cell proliferation. In general, QBMM+ turmeric provided greater cell proliferation compared to that observed using other membranes. The results of the present study suggest that the membranes produced are promising materials for application in tissue engineering, favoring their manufacturing and optimal use.Item Caracterização e testes clínicos de dermocosmético antimicrobiano para coberturas de feridas cutâneas de difícil reparo(Universidade Brasil, 2022) Serra, Thallita Karollaine de Queiroz Pereira; Tim, Carla Roberta; Santos, Laurita dosThis study aimed to characterize an ozonized dermocosmetic and verify in clinical trials its antimicrobial and repairing potential in difficult-to-repair skin wounds. For the production of dermocosmetic, sunflower oil was used. The acid number, peroxide number, and iodine number were analyzed using The American Oil Chemists' Society method. The clinical test was developed with patients with difficult-to-repair wounds and approved by the ethics and research committee (nº 4, 246, 236). Then the patients were randomly allocated into Group 1, treatment with ozonized dermocosmetics, and Group 2, treatment with conventional dressing. Treatments were performed three times a week for 12 weeks. The characterization results showed that the dermocosmetic had an acid index of 17.68± 0.03 mg KOH/g, an iodine index of 86 ± 1 Cgl/g, and a peroxide index of 266.52 meq/kg. In the macroscopic evaluation after 12 weeks, an improvement in the appearance of the lesions was observed, such as an increase in granulation tissue, absence of odor, and a decrease in slough and presence of liquids. There was also complete repair in some injuries, so this group showed stimulation of the repair process with a reduction of 67 to 100% in the area of injury. Initially, 38 bacteria from 13 different species were identified in both groups. The bacteria were classified according to Gram staining. Thus, Gram-negative corresponds to 76.31%, with Pseudomonas aeruginosa being the most diagnosed. Gram-positives represent 23.69%, with Enterococcus faecalis being the most common. The identified microorganisms were evaluated using the antibiogram, divided into sensitive and resistant. Furthermore, it was observed that the use of the ozonized dermocosmetic promoted an antibacterial effect on the lesions of 13 patients. The ozonated dermocosmetic group had a higher frequency of patients without pain after treatment. However, in the conventional dressing group, after 12 weeks, there was a small change in the wounds without significant improvement. Only one patient showed the absence of microorganisms after the treatment period. Furthermore, 37.50% of the patients in the conventional dressing group had an increase in the lesion area, and the pain level was considered moderate. It is concluded that the dermocosmetic group promoted an antimicrobial effect and thus stimulated the repair process of cutaneous wounds.Item Influência do tempo de ozonólise no processo de ozonização de óleo vegetal utilizado para o reparo de pele(Universidade Brasil, 2023) Tarocco, Juliana Carolina; Tim, Carla Roberta; Santos, Laurita dosLesões cutâneas, que são interrupções na continuidade das camadas da pele, afetam significativamente a qualidade de vida tanto de humanos quanto de animais. Essas lesões também implicam em altos custos para a rede de saúde pública e gastos consideráveis em clínicas e hospitais veterinários. Dado esse contexto, este estudo objetivou avaliar a influência do tempo de ozonólise durante o processo de ozonização do óleo de girassol com fluxo de O2 de 3L/min em uma concentração de O3 = 41,6 g/m3 no estímulo do reparo de feridas dérmicas em animais. Foram utilizados 35 ratos Wistar machos, que tiveram feridas induzidas por um punch de 10 mm. Estes foram distribuídos em grupos: controle (sem tratamento), e grupos tratados com óleo de girassol ozonizado por 30, 60, 90 e 120 minutos. Em cada lesão, foram administrados 80 uL do óleo ozonizado topicamente por 10 dias consecutivos. Na avaliação macroscópica, nenhum animal apresentou sinais de odor, necrose, fibrose ou presença de líquido na lesão. A análise do índice de reparo indicou que todos os grupos tiveram a mesma redução percentual da área lesionada. Similarmente, a análise histológica não mostrou diferenças no processo de reparo entre os grupos. A espectroscopia Raman diferenciou com sucesso a pele lesionada da pele saudável, mas não conseguiu distinguir entre o grupo controle e os grupos tratados com óleo ozonizado. Conclui-se que o tempo de ozonização do óleo, até 120 minutos, não alterou de forma significativa as estruturas químicas da pele nem estimulou seu reparo.Item Aplicação subcutânea da Ozonioterapia no processo de reparação tecidual em modelo experimental de feridas cutâneas em ratos(Universidade Brasil, 2023) Muniz, Tânia Maria Quintal; Garcia, Lívia Assis