Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Resultados da Pesquisa
Item Utilização de quitosana e piperina na alimentação de animais: análise da eficácia no reparo de lesões cutâneas em ratos(Universidade Brasil, 2024) Carvalho, Conceição de Maria Aguiar; Costa, Adriana Pavinatto da; Tim, Carla RobertaThe skin, known as the largest organ in the human body, stands out for its unique role in maintaining the integrity of the organism. Disruptions in the epithelial layers are called skin lesions or wounds. Wound repair consists of a mechanism consisting of the phases of hemostasis and inflammation, proliferation and remodeling, starting immediately after the injury occurs. The need for efficient therapy to care for a population affected by injuries is a growing challenge that demands innovative, efficient and affordable strategies. In this scenario, the present study aims to the evaluation of the association of the antimicrobial and reparative action of Chitosan (Chi) with the anti-inflammatory action of Piperine (PIP) in the process of repairing skin lesions. To this end, lyophilized microparticles of Chi and PIP were produced and characterized for subsequent administration to animals with lesions induced via gavage. After freeze-drying, 2.4g of QUI and 2.7g of Chi/PIP were obtained. The spectra obtained through Fourier Transform Infrared Spectroscopy (FTIR) showed vibration bands characteristic of the chemical groups that form the material and corroborate the literature. Scanning electron micrographs (SEM) of the powdered samples showed particles with relatively rough surfaces, while the freeze-dried samples presented thinner surfaces with greater surface area. Both materials do not present a thermal degradation process at temperatures below 60ºC, indicating that they remain active (not degraded) when subjected to animal body temperature. The qualitative analysis of the lesions, obtained from in vivo results, revealed no signs of necrosis, odor or liquid in the lesion cavity, nor did it indicate the presence of fibrosis. The morphometric analysis of the wound repair index showed no statistically significant difference between the experimental groups in all periods studied. Likewise, through histological analysis of the injured tissue, it was not possible to observe significant changes between the experimental groups.Item Análise qualitativa do cortisol salivar por espectroscopia no infravermelho(Universidade Brasil, 2020) Carvalho, Conceição de Maria Aguiar; Mendes, Thiago de OliveiraWidely used to diagnose and monitoring of different diseases, such as Addison's disease, Parkinson's disease and stress association, the cortisol is a glucocorticoid, belonging to the steroid family has a special interest for the medical community. The present work includes the study of infrared spectrum of the cortisol molecule and performs a qualitative analysis of the level of salivary cortisol. For this, the infrared spectrum of the cortisol molecule was obtained by computational methods, based on the Theory of Functional Density, using Avogadro, Gaussian and VEDA software. The stretching of the double CO in 1744 cm-1 e 1776 cm-1 bonds as highlights as a more intense region of the infrared spectrum of the cortisol molecule. A set of ways of using major vibrators in 1776, 1744, 1664, 3824, 3688, 3064, 3096, 1440, 1248, 1120, 1296, 928 e 888 cm-1 has been suggested. The experimental spectra of the analytical standard of cortisol, artificial saliva and artificial saliva with cortisol addition was obtained in the reflectance mode with ATR accessory. The theoretical spectral profile was compared with the experimental values of the vibratory modes. Finally, an unsupervised analysis of a set of artificial saliva with and without cortisol addition evidenced as vibrational bonds in 2912, 1706 and 1642 cm-1 as vibratory markers of salivary cortisol, showing that a infrared spectroscopy can be used for evaluation qualitative analysis of salivary cortisol levels.