Navegando por Autor "Macedo, Daniela Bezerra"

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    Influência da Terapia por Fotobiomodulação no tratamento de condições inflamatórias pulmonares e seu impacto sobre o Covid-19
    (Universidade Brasil, 2021) Macedo, Daniela Bezerra; Tim, Carla Roberta; Garcia, Lívia Assis
    Today we are facing a pandemic that continually causes high rates of deaths and catastrophic and countless economic and psychosocial consequences. Thus, this period requires a rapid search for viable procedures that can allow us to use safe and noninvasive clinical tools as prophylactic or even adjuvant methods in the treatment of COVID-19, thus constituting an important challenge to the medical clinic. Evidence shows that photobiomodulation therapy (PBMT) is shown to attenuate the inflammatory process and reduce respiratory disorders similar to acute lung injury (ALI), complications associated with infections, such as that caused by the new Coronavirus (SARS-CoV-2). Thus, the aim of this study was to evaluate the influence of laser PBMT on infrared length in the treatment of ALI, one of the main critical complications of COVID-19 infection, in an experimental model of rats. Twenty-four male Wistar rats were randomly allocated into three experimental groups (n = 8): Control (CG); LPA (LPA); LASER-treated LPA (LPAL). For the treatment, a laser equipment (808 nm; 30 mw; 1.8 J) was applied at three points (anterior region of the trachea and in the venereal regions of the thorax bilaterally) in the period of 1 and 24 hours after lpa induction. Descriptive histopathological analysis, lung injury score, analysis of the number of inflammatory cells, interleukin 1 β (IL-1β) and interleukin 10 levels (IL-10) were performed for treatment evaluation. In the results, it was possible to observe that the treatment reduced inflammatory infiltrates, there was thickening of the alveolar septum and the pulmonary injury score when compared to the LPA group. In addition, laser PBMT showed lower immunoexpression of IL-1β, however, it did not alter the levels of pulmonary IL-10. Thus, it can be concluded with this experimental study that infrared laser PBMT was efficient in attenuating ALI, one of the main complications of COVID-19, since it was able to reduce the inflammatory process, as well as increase the production of anti-inflammatory cytokines.
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    Membranas de quitosana associada a cúrcuma: produção, caracterização e avaliação da biocompatibilidade e efeitos antimicrobianos
    (Universidade Brasil, 2024) Macedo, Daniela Bezerra; Assis, Lívia; Pavinatto, Adriana
    Skin wounds cause pain, functional impairment and quality of life and are associated with significant economic impact. Chitosan and turmeric have properties that have the potential to promote the repair of skin wounds. However, the relationship between medium molar mass chitosan associated or not with turmeric, as well as the physicochemical properties and biological effects have not yet been elucidated. Thus, the objective of the present study was to produce, characterize and evaluate, in vitro, the cytotoxicity of a membrane based on medium molar mass chitosan associated or not with turmeric in a fibroblast line (L929), as well as the antimicrobial potential for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Three chitosan based membranes of medium molar mass were produced using the casting (solvent evaporation) thin film formation technique: 1% chitosan-based membrane (m/v; QM); chitosan associated with 30% glycerol (m/m in relation to the mass of chitosan; QMG); chitosan associated with 30% glycerol and 1.5% turmeric (m/m in relation to the mass of chitosan; QMGC). To characterize the physicochemical and morphological properties of the membranes, infrared absorption spectroscopy (FTIR), contact angle and thickness measurements were used. To evaluate cell viability/cytotoxicity, L929 cells were used at a concentration of 1x104 using the alamarBlue® and Picrogreen assay; To evaluate the antimicrobial potential, strains of Gram-positive bacteria S. aureus and Gram-negative bacteria E. coli were used at a concentration of 1 x 108 by evaluating the inhibition halo. The results show that all membranes were sustainable, homogeneous and flexible, with the QMG and QMGC membranes being the most flexible and most hydrophilic compared to the QM membrane. Vibrational bands were observed for chemical groups of chitosan and glycerol in all membranes. Regarding the evaluation of cytotoxicity, all membranes showed higher values of metabolic activity/viability, as well as a greater proliferation of fibroblast cells in the experimental period of 72 hours when compared to 24 hours of culture. Furthermore, all membranes presented an inhibition halo to the tested microorganisms, with the QMGC membrane promoting the greatest inhibition halo. With the results obtained in the present study, it is possible to confirm the reparative potential of chitosan-based membranes of medium molar mass, as well as their antimicrobial potential, with emphasis on the membrane associated with turmeric. However, further studies are needed to elucidate the use of these membranes as potential biomaterials for bioactive wound dressings.