Navegando por Autor "Assis, Lívia"

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Avaliação da atividade antimicrobiana da terapia fotodinâmica no tratamento da microbiota bucal
(Universidade Brasil, 2022) Moreti, Lucieni Cristina Trovati; Tim, Carla Roberta; Assis, Lívia
Photodynamic Therapy (PDT) is based on the interaction of light with a photosensitizer capable of promoting microbial destruction. In the present work, the antimicrobial activity of aPDT was evaluated in vitro using two photosensitizers (phenothiazine and annatto) and different times of treatment of oral biofilm, as well as microorganisms were identified and bacterial resistance and sensitivity to certain antibiotics were evaluated. For this, antimicrobial samples of oral biofilm (tongue) were collected from patients without and with pneumonia admitted to the ICU, who were intubated for a period of at least 24 and at most 48 hours, using swabs soaked in saline solution (NaCl 0.5 %) and later cultivated in agarized media. Samples from patients without pneumonia were randomized into: non-pneumonia control group (NP - C): microorganisms without intervention; non-pneumonia chlorhexidine group (NP - CLX): used 0.12% chlorhexidine; non-pneumonia group red PDT (NP - PDTV): used aPDT using 630 nm LED and phenothiazine photosensitizer; non-pneumonia blue PDT group (NP - PDTA): used the use of aPDT using 450 nm LED and annatto photosensitizer, both using a single application. And control pneumonia group (P - C): microorganisms without intervention; group with chlorhexidine pneumonia (P ¬ CLX): used 0.12% chlorhexidine; group with red PDT pneumonia (P - PDTV): used the use of PDT using 630 nm LED and phenothiazine photosensitizer; group with blue PDT pneumonia (P - PDTA): used the use of PDT using 450 nm LED and annatto photosensitizer. They were irradiated with the following parameters: red LED (λ630 ± 10ηm) or blue LED (λ450 ± 10ηm), both at 100 mW power and treated for 150, 300 or 600 seconds. After 24 hours, colony forming units (CFU) and antibiogram were counted. The results showed that the NP – CLX and P – CLX groups promoted total CFU reduction. The PDT groups in oral biofilm without and with pneumonia showed that the CFU reduction was dependent on the treatment time, being better at 600 seconds of treatment. Through the genetic code, Gram-negative microorganisms such as Pseudomonas aeruginosa and Gram-positive microorganisms such as Bhargavaea sp., Desemzia incerta, Kocuria rosea, Micrococcus sp. ,Micrococcus luteus, Agrococcus carbonis, Microbacterium paraoxydans, Corynebacterium sp., Dietzia aurantiaca, Enterococcus faecalis and Staphylococcus saprophyticus.The antibiogram performed evaluated the multiple antimicrobial resistance index (IRMA) characterizing multiresistance to all. Conclusion: Despite being composed of multidrug-resistant microorganisms, aPDT showed potential for the treatment of oral biofilm in patients with and without pneumonia.
Avaliação dos efeitos fototérmico do laser de diodo λ1470 nm na pele em modelo experimental de ratos.
(Universidade Brasil, 2024) Godoi, Silvana Aparecida Girão de; Assis, Lívia; Tim, Carla Roberta
The subdermal high-power diode laser is a therapy that has been frequently used as a treatment for skin aging. However, there is a need for studies to verify morphological and molecular changes in the skin triggered by this light technology. Thus, the objective of the present study was to evaluate the photothermal effects of diode laser wavelength (λ) 1470 nm on the skin of rats. 20 male Wistar rats were used, randomized into 2 groups: placebo laser (LP): treatment with subdermal laser turned off; active laser (LA): subdermal laser treatment was used. For therapy, high-power diode laser equipment was used (λ = 1470 nm; P = 2 W; beam area = 240 μm; beam divergence in rad: 0.227; continuous mode), applied in 5 subdermal vectors in the region of the animal's back in an area of 24 cm2 depositing an average of energy of 85 J/cm2, in a single session and the tissue was evaluated 15 days after the intervention. To evaluate the effects of the treatment, histological, morphometric analyzes and immunoexpression of transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) were performed. The results demonstrated that active laser animals showed a better arrangement of collagen fiber bands and an increase in the thickness of the dermis and the number of vessels. Furthermore, animals treated with active laser showed an increased immunoexpression of TGF-β and VEGF compared to placebo. Thus, we can conclude that the subdermal high-power diode laser proves to be a promising option in aesthetic dermatology, as it was capable of increasing vascularization and the expression of factors that enhance skin rejuvenation.
Avaliação in vitro do potencial antimicrobiano e da viabilidade celular de diferentes concentrações de Ozonioterapia
(Universidade Brasil, 2022) Morais, Jynani Pichara; Assis, Lívia; Tim, Carla Roberta
Cutaneous wound is a clinical condition with high prevalence and represents a public health problem. Although ozone therapy (O3), ozone therapy, is widely used in clinical practice due to its antimicrobial and restorative effects, evidence on ideal dosimetries is still scarce. Thus, the aim of the present study was to evaluate and compare, in vitro, the cytotoxicity of different concentrations of ozone therapy in keratinocyte (NOK) and fibroblast (L929) lines, as well as the antimicrobial potential for Staphylococcus aureus (S.aureus) and Escherichia coli (E. coli). NOK and L929 cells were seeded at a concentration of 1x104 and strains of gram-positive bacteria S.aureus and gram negative bacteria E. coli at a concentration of 1x108 and divided into 7 experimental groups treated with ozone at doses of 0 (G0), 5 (G5), 10 (G10), 20 (G20), 30 (G30), 40 (G40) and 50 μg/mL (G50). The effect of ozone on cell viability/cytotoxicity was determined using the alamarBlue® assay, the Live/Dead® assay, and morphometric analysis of the percentage of live and dead cells. The effect of the gas on the microorganisms was evaluated by counting the colony forming unit (CFU). The results obtained show that ozone at concentrations of 5, 10 and 20 μg/mL presented the highest viability values of NOK (keratinocytes) and L929 (fibroblasts) cells, as well as a higher density and percentage of live cells in the experimental period of 72 hours when compared to other concentrations. However, it was not possible to observe the difference between the percentage of dead cells between the experimental groups. Furthermore, ozone promoted a reduction in CFUs at all concentrations tested. With the results obtained in the present study, it is possible to confirm the reparative potential of ozone therapy in a dose-dependent response, as well as its antimicrobial potential. However, studies are needed to elucidate the molecular mechanisms by which it exerts such biological effects.
Comportamentos e ações de vida diária e sua relação com a infecção pelo coronavírus
(Universidade Brasil) Carvalho, Jucilene Santos de; Assis, Lívia; Tim, Carla Roberta; Santos, Laurita dos
Efeitos da fotobiomodulação associada ou não ao Biopolímero de Fibrina Heterólogo no processo de reparo tecidual de lesões cutâneas de ratos diabéticos
(Universidade Brasil, 2021) Alexandria, Francisco Eugênio Deusdará de; Assis, Lívia; Tim, Carla Roberta
INTRODUCTION: Diabetes mellitus is a general heterogeneous term that classifies metabolic disorders caused by increased glucose levels (hyperglycemia). Among the therapeutic modalities researched in order to accelerate tissue repair, LED photobiomodulation therapy and heterologous fibrin biopolymer stand out. PURPOSE: To evaluate the effects of LED photobiomodulation therapy associated or not with heterologous fibrin biopolymer in the tissue repair process of skin lesions in diabetic rats. METHODOLOGY: Forty-eight adult female Wistar rats (Rattus norvegicus albinus), 60 days old, weighing 230±20 g, from the vivarium of the State University of Piauí, were used. The animals were randomly divided into 4 distinct groups (control group, heterologous fibrin biopolymer group, LED photobiomodulation therapy group, LED photobiomodulation therapy group associated with heterologous fibrin biopolymer), which were treated in two experimental periods (7 and 14 days). Diabetes was induced by the administration of an aqueous solution of 2% alloxan and the animals had their blood glucose assessed using a glucometer and clinical symptoms. The animals underwent two surgical procedures, in the dorsal and cervical regions, the first by means of a skin punch, an area of 2 cm²; and the second a linear lesion measuring 3 cm, in the cervical region. Healing analysis was performed by calculating the contraction of the wound area and evaluating the traction force. RESULTS: The treatments with LED photobiomodulation therapy and heterologous fibrin biopolymer, used alone or in combination, indicated the stimulation of the repair process. The heterologous fibrin biopolymer group showed similar results to the LED photobiomodulation therapy group in wound regression, but showed better healing resistance, suggesting that the LED group has both repair qualities. The LED photobiomodulation therapy group associated with heterologous fibrin biopolymer showed better results in resistance and regression when compared to the heterologous fibrin biopolymer and LED photobiomodulation therapy groups, statistically closer to the control group. CONCLUSION: Based on the findings, it was observed that treatments with LED, BFH or LED associated with BFH acted satisfactorily, favoring the improvement in the tissue repair process, suggesting that these treatments, isolated or associated, were effective in stimulating the healing process in diabetic rats.
Efeitos da fotobiomodulação associada ou não ao Biopolímero de Fibrina Heterólogo no processo de reparo tecidual de lesões cutâneas de ratos diabéticos
(Universidade Brasil, 2021) Alexandria, Francisco Eugênio Deusdará de; Tim, Carla Roberta; Assis, Lívia
Efeitos do agulhamento seco no tratamento da dor de pacientes com disfunções temporomandibulares
(Universidade Brasil, 2021) Castro, Tânia Ecí Santi; Assis, Lívia; Tim, Carla Roberta
Temporomandibular disorder (TMD) is a disorder of the masticatory system originating from the masticatory muscles and/or the temporomandibular joint and associated structures. This dysfunction is responsible for the discomfort of millions of people around the world, decreasing their quality of life and restricting social interaction. In this perspective, this study aimed to identify the potential of dry needling in the treatment of TMD through a systematic literature review and evaluation of the use of deep dry needling for the treatment of TMD. For this, articles were searched in the PubMed and Virtual Health Library (LILACS, MEDLINE and SciELO) databases with the descriptors in English "temporomandibular disorder dry needling", or "temporomandibular disorder myofascial dry needling", or "temporomandibular disorder dry needling. Using a combination of descriptors as the search strategy. Nine studies were considered. One study compared superficial and deep dry needling. Five studies used only deep dry needling, another three studies did not specify the type of needling used, and only one article compared deep with superficial dry needling. of cases with three patients with chronic TMD diagnosed through clinical examination based on the Research Diagnostic Criteria for Temporomandibular Disorders (RDC / TMD) and undergoing 4 seasons needling dry, once a week. Deep dry needling was used in the g-points tile in the masseter muscles and through temporal needles 0.20 x 13 mm. After 4 weeks of treatment, it was observed that pain intensity and hospital anxiety and depression scale scores decreased and increased mouth opening. Based on the results, it can be suggested that dry needling promoted a remission of painful symptoms and an improvement in mandibular range of motion after four weeks of treatment.
Membranas de quitosana associada a cúrcuma: produção, caracterização e avaliação da biocompatibilidade e efeitos antimicrobianos
(Universidade Brasil, 2024) Macedo, Daniela Bezerra; Assis, Lívia; Pavinatto, Adriana
Skin wounds cause pain, functional impairment and quality of life and are associated with significant economic impact. Chitosan and turmeric have properties that have the potential to promote the repair of skin wounds. However, the relationship between medium molar mass chitosan associated or not with turmeric, as well as the physicochemical properties and biological effects have not yet been elucidated. Thus, the objective of the present study was to produce, characterize and evaluate, in vitro, the cytotoxicity of a membrane based on medium molar mass chitosan associated or not with turmeric in a fibroblast line (L929), as well as the antimicrobial potential for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Three chitosan based membranes of medium molar mass were produced using the casting (solvent evaporation) thin film formation technique: 1% chitosan-based membrane (m/v; QM); chitosan associated with 30% glycerol (m/m in relation to the mass of chitosan; QMG); chitosan associated with 30% glycerol and 1.5% turmeric (m/m in relation to the mass of chitosan; QMGC). To characterize the physicochemical and morphological properties of the membranes, infrared absorption spectroscopy (FTIR), contact angle and thickness measurements were used. To evaluate cell viability/cytotoxicity, L929 cells were used at a concentration of 1x104 using the alamarBlue® and Picrogreen assay; To evaluate the antimicrobial potential, strains of Gram-positive bacteria S. aureus and Gram-negative bacteria E. coli were used at a concentration of 1 x 108 by evaluating the inhibition halo. The results show that all membranes were sustainable, homogeneous and flexible, with the QMG and QMGC membranes being the most flexible and most hydrophilic compared to the QM membrane. Vibrational bands were observed for chemical groups of chitosan and glycerol in all membranes. Regarding the evaluation of cytotoxicity, all membranes showed higher values of metabolic activity/viability, as well as a greater proliferation of fibroblast cells in the experimental period of 72 hours when compared to 24 hours of culture. Furthermore, all membranes presented an inhibition halo to the tested microorganisms, with the QMGC membrane promoting the greatest inhibition halo. With the results obtained in the present study, it is possible to confirm the reparative potential of chitosan-based membranes of medium molar mass, as well as their antimicrobial potential, with emphasis on the membrane associated with turmeric. However, further studies are needed to elucidate the use of these membranes as potential biomaterials for bioactive wound dressings.
Plasma rico em plaquetas: investigação da efetividade de diferentes protocolos para aplicação em pé diabético
(Universidade Brasil, 2020) Cruz, Cecília Penha Dall'orto; Tim, Carla Roberta; Assis, Lívia
Diabetes mellitus (DM) is characterized as a metabolic disorder of persistent hyperglycemia. Diabetic foot is one of the first complications identified in diabetic patients and causes major negative manifestations in their quality of life. These wounds are prone to infection, and when, moderate or severe, they suffer some level of limb amputation. Platelet-rich Plasma (PRP) is a biological product produced from an autologous blood centrifugation process, allowing plasma extraction with a concentration of three to five times higher than that of platelets without basal blood. In this perspective, this study aimed at a systematic review to assess the effectiveness of different PRP recovery protocols for wound treatment and to verify the feasibility of PRP recovery protocols by varying the strength / speed and the centrifugation time. For this purpose, a search was performed in the articles of the Virtual Health Library (VHL) with the descriptors in English and Portuguese “chronic ulcers, Platelet-rich plasma, Deep Soft Tissue Repair, Chronic Wounds, Wound Healing, Regenerative Medicine, Therapeutics and wound healing. Use a combination of descriptors as a search strategy. The publication period was delimited between the years 2010 to 2019. Other productions that were not included in the scientific article category were excluded, such as thesis, dissertations as well as inconclusive work, case reports, review of narrative literature and study on animals. 66 articles were selected, after exclusion, only 13 composite articles or corpus of the study. In 61.5 % of the studies, a PRP autologous gel application was used; and 38.4 % opted for injecting PRP, either subcutaneously or intradermally. Total of 13 studies analyzed, all proven that the PRP assist in the healing and tissue regeneration process. In 23 % of the studies analyzed there was an investigation of the pain variable, with improvements in it and in the patient's quality of life. Clinical trials continue to be of great importance for the case in question, as well as the analysis of systematic literature for recruiting information and increasing the occurrence of subjects. PRP has a great optional therapeutic potential in the treatment and healing process of chronic ulcers. Therefore, the second study aimed to evaluate the effectiveness of the reproduction of two PRP recovery protocols, varying strength / speed and centrifugation time. Twenty trained volunteers were recruited, with platelet count within the reference range 150 - 450.10/³ uL. Two different centrifuges were used to prepare the PRP: 1) CE1161 digital laboratory model centrifuge, adjustable speed - 0 to 5000 rpm with a radius of 14 cm and fixed rotor; with 10 patients; 2) Kasvi digital mod. Ce800, adjustable speed from 100 to 4000 rpm with a radius of 14 cm and variable angle, with 10 patients. The present study found that the preparation of the PRP using the highest force / speed and the centrifugation time, promoting the concentration of coatings statistically higher when compared to the protocol of lower force / speed. It was not possible to reproduce the protocols in different centrifuges. The results pointed out beyond the force of gravity and the centrifugation time, or the rotor angle can also influence the preparation of the PRP.
Uso da Ozonioterapia em fungos causadores de Onicomicoses: estudo in vitro
(Universidade Brasil, 2024) Del Castilo, Denise Vivianni Ferreira; Assis, Lívia; Tim, Carla Roberta
: Onychomycosis is a common fungal infection of the nails, predominantly caused by dermatophytes such as Trichophyton rubrum (T. rubrum) and Microsporum gypseum (M. gypseum) and is a notoriously difficult condition to treat. Thus, the objective of the present study was to evaluate and compare, in vitro, the effectiveness of different times of ozone therapy on the main fungi that cause onychomycosis. Dermatophyte fungi T. rubrum (ATCC 28188) and M. gypseum (ATCC 24102) were incubated at 28° C for 14 days. Subsequently, the fungi were divided into 9 experimental groups according to the experimental time: Control Group (CG): the fungi were cultivated and did not receive any treatment; Cultivated fungi and received ozone therapy for a time of 2 min (G2”); 4 min (G4”); 6 min (G6”); 8 min (G8”); 10 min (G10”); 12 min (G12”); 14 min (G14”) and 16 min (G16”). The ozone concentration used was 2 µg/mL, oxygen flow of 1/4 L/min and applied dosages of 157, 314, 451, 628, 785, 943, 1100 and 1257 mg/m2, respectively. The main results show that the use of topical ozone therapy was effective in reducing the germination percentage of T. rubrum and M. gypseum in all experimental periods, presenting complete eradication with a treatment time of 6 min for T. rubrum and 14 min for M. gypseum. In conclusion, ozone therapy used topically, with emphasis on the treatment time of 6 min for T. rubrum (628 mg/m2) and 14 min (1257 mg/m2) for M. gypseum, promoted antifungal action on the main dermatophytes responsible for critical complications of onychomycosis, and may be proposed as a adjuvant in dermatological treatments.
Utilização de gerador de alta frequência no tratamento do envelhecimento cutâneo
(Universidade Brasil, 2022) Santos, Ana Keila Ferreira dos; Tim, Carla Roberta; Assis, Lívia; Sávio, Rodrigo
Given the exposure to damage that affects the essential components of the skin, it is necessary treatment to minimize all the weakness that occurred. Today, aesthetic dermatology dispenses with several resources to improve cell revitalization, thus delaying facial aging. In this context, the present study aims to evaluate the effect of the high frequency generator in the treatment of skin aging in adult women. The study included 26 participants aged between 50 and 60 years. The candidates received high frequency generator application 3 times a week for 8 weeks. The evaluation occurred through photographs and evaluation instruments, such as self-esteem assessment, Rosenberg scale, evaluation of perception with treatment, satisfaction with treatment, overall aesthetic improvement scale, modified Fitzpatrick scale. With the results, mean age of the participants 54.4 years, marital status, 47% are married, 23% reported having completed higher education and 19% incomplete elementary school. As for the occupation exercised, 15% are from the home, and the others perform other work activities. Regarding the clinical and dermatological history, 92% are in climacteric period. Half of the participants practice physical activities, and 77% of them do not smoke. Most have mixed skin, use sunscreen sometimes and the most used sun protection factor is SPF 30 represented by 42%. Most candidates have type III skin phototypes. Regarding the perception and satisfaction with the treatment, both evaluated satisfactorily and positively by the volunteers. The overall aesthetic improvement, assessed by the Global Aesthetic Improvement Scale, was significant, in which 50% of the candidates presented a much better scale, 42% of much better and 8% of the best. The evaluation of nasolabial wrinkles by the Modified Fitzpatrick Wrinkle Scale showed that HF promoted statistical improvements after 8 weeks, this also happened for periorbital wrinkles on both sides. Thus, it is observed that the high frequency generator can be a therapeutic resource for the treatment of skin aging of the face, because it presented satisfactory results in the skin, allowing a good appearance and increasing the self-esteem of the candidates.