Avaliação in vivo dos possíveis efeitos genotóxicos e antimicrobiano de scaffolds a base de poli (butileno adipato-co-tereftalato) /polipirrol com nanohidroxiapatita para regeneração óssea

Abstract

An increasingly frequent trend in tissue engineering research is the development of nanoscale nanofiber scaffolds. It is then proposed to manufacture polymeric scaffolds using electrospinning (PBAT 12%, PBAT 12% / PPy 1%), and homogeneous deposition of nHAp nanocomposites on the surface of the fibers using electrochemical electrodeposition (PBAT 12% / PPy 1 % / nHAp), for bone regeneration. The electrospinning process was carried out with 12 13 kV, 1.2 mm needle, 0.3 ml / h infusion pump and 10-12 cm distance from the counter electrode. Subsequently, nHAp was directly electrodeposited on PBAT / PPy scaffolds using a classic three-point device. The scaffolds were characterized by SEM / EDS scanning electron microscopy, FTIR by ATR, XRD, tests to evaluate possible in vivo genotoxicity effects (MN and Comet), and antibacterial activity. In XRD the size of the scaffold crystallite with deposited nHAp was 72.6 nm. In the MN test the experiment consisted of 05 animals (n = 5), with four groups: GI - PBAT / PPy / nHAp, GII-PBAT / PPy, Positive control - Cyclophosphamide at a dose of 50mg / kg intraperitoneally and the Control negative - distilled water. Blood was collected from the tail of rats for the comet test. In the micronucleus test, bone marrow cells were collected from the rat femur, showing that the scaffold did not induce DNA damage and genotoxicity in the cells. In antimicrobial tests, scaffolds are 99.8% effective in antibacterial activity (bacteria Pseudomonas aeruginosa). In conclusion, scaffolds have no genotoxic effect, and this new biomaterial presents great potential for possible use in vivo and in bone repair.