Estudo dos efeitos bioquímicos da luz azul na pele humana por Espectroscopia Raman Confocal

Abstract

Introduction: Raman spectroscopy is a recent and non-invasive technique that can be used to assess a biochemical structure of the skin under different conditions. This technique is already useful in several contexts, although additional studies are still being published. The effect of blue light on the skin and its clinical functions, such as photoaging, have been described in the literature. In addition, the light component of solar radiation is shown to be a risk factor for several diseases, such as melanoma, such as skin patches, pigmented nevi and other skin lesions. Thus, an investigation of the effects of blue light using Raman spectroscopy is useful and necessary for the current scenario. Objective: To understand biochemical changes in human skin due to the incidence of blue light, using Raman Conforcal Spectroscopy. Method: This is a monocentric, retrospective study, based on the analysis of a database with prospective collection carried out in accordance with DermoProbes' operational procedure. Results: After irradiation of the blue LED for 15 minutes (100 J / cm2), damage was observed in the stratum corneum, which corresponds to disulfide, tyrosine, phenylanine and beta-carotene bonds, and damage to Amide I in the viable epidermis. After subsequent irradiation, in the same area, for another 15 minutes (totaling 200 J / cm2), an increase in the damage to the viable epidermis was observed. For this irradiation the signatures in the bands of felilalanine, L-glutamano, valine, lipoic acid and beta-carotene stand out. When compared to irradiated skin with virgin skin for 45 minutes (corresponding to 3 incidences of 15 minutes, totaling 300 J / cm2), it was observed that the separation by cluster of virgin and irradiated skin is complete, for this irradiation, it stands out as signatures of oleic acid and Amide III. It was found that different doses act on different molecular groups of the stratum corneum and dermis. When the damage is intense as proteins are the most affected and when the damage is less, the antioxidant beta carotene and amino acids such as tyrosine and phenylalanine are activated. Conclusion: The present study, from the analysis of Raman spectroscopy, as the main biochemical changes in the skin after different intensities of blue light irradiation. From the results obtained, it was possible to verify that the damage to the biochemistry of the skin increases as the exposure to blue light increases, at a wavelength similar to the visible component of sunlight.