Efeitos da fotobiomodulação por laser de baixa intensidade associado ou não ao extrato vegetal Abelmoschus Esculentus Linn sobre a inflamação aguda do tendão calcâneo induzida por colagenase em ratos Wistar

Abstract

INTRODUCTION: In the current literature, the association between electromagnetic resources (photobiomodulation (FBM) by low intensity laser (LBI) and / or by emitting diode light (LED) in the infrared electromagnetic spectrum and plant extracts (ointments / gels) in the treatment of tendinitis are still scarce, however, it is believed that both can help in resolving the inflammatory process OBJECTIVE: To evaluate the effects of low intensity laser photobiomodulation (FBM) with or without the “Abelmoschus Esculentus Linn” extract, in treatment of calcaneus tendon tendonitis experimentally induced via collagenase in Wistar rats METHODOLOGY: 40 male Wistar rats submitted to the experimental model of tendinitis via collagenase application on the right paw.The animals were divided into 4 experimental groups: control group (GC), extract (EX), photobiomodulation (FBM) and the combined treatment group (FBM + EX), which were treated in a controlled manner during the period experimental period of 7 and 14 days. The variables studied in this study were: inflammatory cells, fibroblast count and the percentage of collagen fibers (types I and III). RESULTS: It was observed in the study in the inflammatory cell count that the FBM (0.010) and AS (0.043) groups showed a significant reduction in the number of inflammatory cells in the intragroup analysis. Regarding the findings in the fibroblast count, it was proved in the intragroup analysis that the CG (0.0035) showed a significant increase in the number of fibroblasts, while the FBM group (0.013) showed a significant reduction in the proliferation of fibroblasts. In the intergroup analysis, the experimental groups (EX, FBM and AS), showed a significant increase in the proliferation of fibroblasts when compared to the CG in the experimental times studied. Finally, in the analysis of the percentage of collagen fibers, it was found that the FBM group had the best results on collagen proliferation (types I and III). CONCLUSION: It is concluded that the extract Abelmoschus Esculentus Linn, when applied in isolation, does not optimize the proliferation of type I collagen in the expected phase of the calcaneus tendon repair process. However, it has been shown that FBM is able to assist the proliferation of fibroblasts and induce the proliferation of type I collagen, reducing the proliferation of type III collagen in the late phase (14 days).