Efeitos da Ozonioterapia doméstica em modelo experimental Murino de vulvovaginite por Candida albicans

Abstract

In vulvovaginal candidiasis, itching, whitish plaques, leucorrhoea, erythema and edema in the vulva and vagina are commonly observed or observed. For common treatment of candidiasis, antifungals are used in the form of pills or ointments applied directly to the area. Antimycotics can have undesirable side effects and are expensive. A proposal of low cost, simple, easy to access and with few side effects for the treatment can be based on the medicinal use of ozone. The aim of the study was to evaluate the effects of medicinal domestic ozone therapy on C. albicans through in vitro and in vivo assays using the murine experimental model of vulvovaginitis. The experiment was conducted in vitro by cultivating Candida albicans strains for 24 h at 37 °C in solid medium Agar Sabouraud Dextrose. In the plates used for control, 10 µL of the cell suspension of C. albicans were seeded, with a calibrated loop, in 10 mm Petri dishes, containing solid medium Agar Sabourand Dextrose (Oxoid). These plates have not been subjected to ozone exposure. The in vivo experiment used 45-day-old female C57/BL6 mice. Diluted in 0.2 ml of distilled water at a dose of 2mg/kg/day for 7 days. After suppression of ovarian activity, 20 µL of Candida albicans suspension in sterile PBS, at a concentration of 105 yeast cells, was inoculated in a single dose and under anesthesia. The distribution took place in 4 groups of animals: Absolute Control Group: Not submitted to infection by the pathogen. Control Group: Subjected to C. albicans infection, but not treated. Ozone Therapy Group: Subjected to pathological agent infection and treated with medicinal ozone therapy. Wash Group: Submitted to infection and later treated with vaginal washing. Flagged as control group. For the Ozone Therapy Group, the vulvovaginal lavage procedure was performed once a day for three days. Promptly before the sacrifice of the rodents, the procedure for collecting the vaginal lavage was performed. 50 µL of sterile PBS was instilled into the vagina of each animal, using a micropipettor and 100 µL tip, and aspirated immediately afterwards. The data obtained from the CFU were analyzed using descriptive and analytical statistics using the ANOVA test, using the Tukey test as posts hoc, with alpha set at p>0.05. It was found that the control plate had a higher amount of CFU when compared to plates exposed to ozone in the air. Exposure for 600 s was able to reduce 98.89% the number of CFU when compared to control plates. The suspensions ozonized for 60 s and 150 s did not show cell growth, obtaining a difference of 100% when compared to the control plate. The amount of UFC counted in ozonized suspensions. The ozone gas showed great efficacy on the proliferation of C. albicans in vitro. Best results observed were with application in liquid medium. Ozone therapy was able to significantly reduce the infection in the experimental model used. In experiments conducted in vivo, the number of CFU/ml found was lower in the group treated with ozone.