Pós-graduação
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/35
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2 resultados
Resultados da Pesquisa
Item Avaliação do efeito do extrato aquoso da casca do caule de “Ximenia Americana L.” incorporado à hidrogel à base de gelatina metacrilato (gelma) no processo de reparo ósseo induzido em ratos(Universidade Brasil, 2023) Leal, Seânia Santos; Costa, Adriana Pavinatto da; Oliveira, Rauirys Alencar deSeveral physiotherapeutic modalities have been studied with the aim of promoting bone repair. In the biomedical context, the use of Biomaterials has been studied to stimulate this process. The present work aims to produce, characterize and evaluate the effect of the stem bark extract of “Ximenia americana L.” incorporated into gelatin methacrylate hydrogel (GelMA) associated or not with photobiomodulation (LED) therapy in the bone repair process induced in rats. To this end, the materials GelMA and GelMA + stem bark extract of “Ximenia americana L.” that were used in in vivo experiments. Animal tests were carried out using 50 male Wistar rats distributed into 5 distinct groups called: control group (CG), GelMA group (GG), Gelma + LED group (GLED), GelMA group + Ximenia americana extract ( GE) and GelMA + Ximenia americana extract _ LED (GELED) group, which were subjected to the induced fracture protocol in the diaphyseal region of the right tibia and treated with light by emission diode 858 nm and energy dose 6J in an established period of 15 and 30 days, being euthanized throughout the same cycle. To verify the effects of the therapies, histological analyzes and Raman spectroscopy were carried out. The results showed that 15 days after inducing injury in the tibia of rats, in the GE group, bone neoformation was higher than in the control group and between the GG and GE groups. In 30 days of the experiment, there was also a difference in bone neoformation between the GELED group and the control group between the GE and control group. There was also a difference between the GG and GE and GELED groups. Given the results, it can be stated that the stem extract of “Ximenia americana L” incorporated into GelMA associated with photobiomodulation from LED is a potentiator of bone repair in an animal model.Item Nanocompósitos à base de hidroxiapatita e nanotubos de carbono como reforço em biopolimero heterólogo de fibrina derivado do veneno de serpente para regeneração óssea(Universidade Brasil, 2021) Gouveia, Daniel Mussuri de; Kido, Hueliton Wilian; Cogo, José CarlosFibrin sealants (BHF), developed from human hematological derivatives, mimic physiological clots, being widely used in general, cosmetic and postoperative surgeries due to their hemostatic and adhesive action. BHF developed from the venom of Crotalus durissus terrificus (rattlesnake) is a heterologous thrombin-like derivative that converts fibrinogen into fibrin. As it is a natural product, in gel form, biodegradable, bioabsorbent, non-toxic and non-immunogenic. On the other hand, hydroxyapatite nanocomposites (nHAp) and carbon nanotubes (NTCs), produced by chemical synthesis assisted by the ultrasound technique, present bioactivity, are not cytotoxic and have a bactericidal effect. The aim of the present work is to evaluate the mixture of hydroxyapatite-based nanocomposites and carbon nanotubes with fibrin sealant as agents in the process of accelerating bone regeneration. The production of the nanocomposites consisted, firstly, in the synthesis of NTCs with multiple walls, followed by their functionalization to the O2 plasma and purification with an acid bath. With this material ready, the composite of nHAp with NTC was produced. Then, this material was added to the BHF and the physicochemical characterization of this mixture was carried out. The pellets formed by these mixtures were analyzed and photographed under scanning electron microscopy (SEM). These mixtures were also tested in MC3T3-E1 cell cultures to verify their toxicity. For this purpose, osteoblast cells were cultured and cell viability was verified using the alamarBlue® e LIVE/DEAD® Viability/Cytotoxicity technique. Experiments were also carried out in rats, inducing bone defect in tibias and verifying bone repair. All data were analyzed using the normality test, and the parametric results were submitted to the two-way ANOVA complemented by Tukey's post-test (p≤0,05). The results showed that there was a good homogeneous distribution of the mixtures and that the most suitable mixture for in vitro tests is that of BFH+nHAp and 1% and 2% carbon nanotubes. The mixture is not cytotoxic and promoted neoformation in the fracture area, demonstrating osteogenic potential in bone fractures.