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URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/35
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Item Análise e otimização dos algoritmos para angiografia por tomografia por coerência óptica e desenvolvimento de Phantom por impressão 3D(Universidade Brasil, 2021) Rodrigues, Karina de Cássia; Almeida, Vilson Rosa de; Amaral, Marcello MagriSkin grafts are surgically applied to repair skin lesions such as burns and extensive necrosis. The success of this surgical procedure is associated with good blood supply in the grafted region. Thus, the assessment of angiogenesis during the tissue repair process is essential for its prognosis. The development of non-invasive evaluation techniques is extremely important for the success of this procedure. One of the promising techniques is Optical Coherence Tomography Angiography (OCT-A), a noninvasive technique that can be used to obtain images of the vascularization of biological tissues. The adoption of this technique as a clinical practice in dermatology involves reducing its cost, and the use of equipment with a low acquisition rate (low cost) is a possible path. Thus, this work aimed to implement and optimize algorithms for obtaining angiography images by optical coherence tomography (OCT-A) for applications in images acquired with low acquisition rate and cost equipment. To test those methods, it is requiring the use of phantom that simulate the behavior of the microvascular system. Thus, this work also aimed at the development of a phantom to simulate a microvascular system using 3D printing technology. Phantoms containing microchannels were designed and printed on polylactic acid (PLA) using a 3D printer by fused filament deposition. These PLA phantoms were imaged with the OCT system (OQLabScope - Lumedica, USA). Seven different OCT-A methods were implemented (HFM, STS, CM, SV, OSV, ISC and UHS-OMAG) and compared against their processing time, signal-to-noise ratio, contrast, and contrast-to-noise ratio. The OSV and CM methods showed better overall performance based on these parameters, but CM shown higher processing time. An optimization of the CM method was proposed in this work, reducing the processing time by 99.2%, a significant gain for the algorithm that presented better performances in contrastItem Desenvolvimento de processo de desinfecção por ozônio e conservação de pele de Lithobates catesbeianus para enxerto cutâneo em modelo animal(Universidade Brasil, 2021) Moraes, Dione Natureza de; Pavinatto, Adriana; Amaral, Marcello MagriSkin replacement is an important step in the treatment of various etiologies. Currently, there are several types of grafts available on the market, each with its own specificities, being used after a clinical study on the patient's health status. The use of bullfrog skin (Lithobates Catesbeianus) has shown promise as a possible skin graft, due to its cellular composition, high concentration of collagen, water and nutrients, biocompatibility, biological interaction with the substrate with the release of tissue biomodulators. In this work, a protocol for the sterilization and preservation of the bullfrog (Lithobates Catesbeianus) skins was developed, and its efficacy as a skin graft in an animal model was evaluated. Sterilization with ozone and conservation in 4 different ozonized solutions (saline solution, tea tree oil, copaiba oil and chitosan) were tested. The protocol of ozone disinfection and conservation in ozonized copaiba oil kept the skin inert to any microorganism for a period of twelve months. The evaluation in animal model was performed by histological analysis and by optical coherence tomography. These analyzes show promising results for future clinical applications, the skin proved to be excellent for use as a xenograft, as there was good graft adhesion, presence of fibroblasts and formation of a newly formed dermis. Compared to the autograft (control), this xenograft showed a greater presence of inflammatory infiltrates, which indicates the beginning of granulation tissue growth.