Desenvolvimento de processo de desinfecção por ozônio e conservação de pele de Lithobates catesbeianus para enxerto cutâneo em modelo animal
Carregando...
Data
2021
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Brasil
Resumo
Skin replacement is an important step in the treatment of various etiologies.
Currently, there are several types of grafts available on the market, each with its own
specificities, being used after a clinical study on the patient's health status. The use
of bullfrog skin (Lithobates Catesbeianus) has shown promise as a possible skin
graft, due to its cellular composition, high concentration of collagen, water and
nutrients, biocompatibility, biological interaction with the substrate with the release of
tissue biomodulators. In this work, a protocol for the sterilization and preservation of
the bullfrog (Lithobates Catesbeianus) skins was developed, and its efficacy as a
skin graft in an animal model was evaluated. Sterilization with ozone and
conservation in 4 different ozonized solutions (saline solution, tea tree oil, copaiba oil
and chitosan) were tested. The protocol of ozone disinfection and conservation in
ozonized copaiba oil kept the skin inert to any microorganism for a period of twelve
months. The evaluation in animal model was performed by histological analysis and
by optical coherence tomography. These analyzes show promising results for future
clinical applications, the skin proved to be excellent for use as a xenograft, as there
was good graft adhesion, presence of fibroblasts and formation of a newly formed
dermis. Compared to the autograft (control), this xenograft showed a greater
presence of inflammatory infiltrates, which indicates the beginning of granulation
tissue growth.
Descrição
Palavras-chave
Xenoenxerto, Enxerto de pele, Lithobates catesbeianus, Substituição de pele, Pele de rã-touro