Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Item Efeito antibacteriano do óleo de girassol ozonizado em diferentes concentrações(Universidade Brasil, 2021) Ficher, Ester; Nunez, Silvia CristinaThe world is currently facing a crisis regarding microorganisms and microbial resistance. The evaluation of alternative antimicrobial therapies has been a constant search in recent decades. Ozonized oil can be an alternative within this field. The present study aims to analyze the antimicrobial efficacy of commercially available ozonated sunflower oil in different concentrations. A gram-positive bacteria Staphylococcus aureus and the gram-negative bacteria Escherichia coli were used. The microorganisms were grown in BHI broth (Brain and Heart Infusion) for 24h. Plates containing BHI agar were prepared for the inhibition halo test with a 3 mm diameter central well and contaminated with 50 µL of bacterial suspension. The wells made in the plates were filled with ozonated sunflower oil in concentrations ranging from 80 mmol-Eq / kg to 1290 mmol-Eq /. The plates were taken to the incubator at 37°C for 24h and the inhibition zone was measured with a millimeter probe, two control groups one with non-ozonated sunflower oil and one with 2% chlorhexidine gluconate solution were also evaluated. The results demonstrated that the efficacy for E.coli increases linearly with increasing concentration, however, only the concentrations of 702 mmol-Eq / kg and 1290 mmol-Eq / kg were significantly different (p<0.004). For S.aureus, the results showed significant differences only in the concentration of 1290 mmol-Eq / kg, but with a similar effect between the intermediate and higher concentrations (p<0.0001). The chlorhexidine group demonstrated to have a more effective action for both microorganisms under the conditions of this study (p<0.001). According to the results ozonized sunflower oil has less antimicrobial effect than chlorhexidine, the result is dependent on the concentration and only concentrations above 702 mmol-Eq / kg showed some efficacy.Item Avaliação in vitro do potencial antimicrobiano e da viabilidade celular de diferentes concentrações de Ozonioterapia(Universidade Brasil, 2022) Morais, Jynani Pichara; Assis, Lívia; Tim, Carla RobertaCutaneous wound is a clinical condition with high prevalence and represents a public health problem. Although ozone therapy (O3), ozone therapy, is widely used in clinical practice due to its antimicrobial and restorative effects, evidence on ideal dosimetries is still scarce. Thus, the aim of the present study was to evaluate and compare, in vitro, the cytotoxicity of different concentrations of ozone therapy in keratinocyte (NOK) and fibroblast (L929) lines, as well as the antimicrobial potential for Staphylococcus aureus (S.aureus) and Escherichia coli (E. coli). NOK and L929 cells were seeded at a concentration of 1x104 and strains of gram-positive bacteria S.aureus and gram negative bacteria E. coli at a concentration of 1x108 and divided into 7 experimental groups treated with ozone at doses of 0 (G0), 5 (G5), 10 (G10), 20 (G20), 30 (G30), 40 (G40) and 50 μg/mL (G50). The effect of ozone on cell viability/cytotoxicity was determined using the alamarBlue® assay, the Live/Dead® assay, and morphometric analysis of the percentage of live and dead cells. The effect of the gas on the microorganisms was evaluated by counting the colony forming unit (CFU). The results obtained show that ozone at concentrations of 5, 10 and 20 μg/mL presented the highest viability values of NOK (keratinocytes) and L929 (fibroblasts) cells, as well as a higher density and percentage of live cells in the experimental period of 72 hours when compared to other concentrations. However, it was not possible to observe the difference between the percentage of dead cells between the experimental groups. Furthermore, ozone promoted a reduction in CFUs at all concentrations tested. With the results obtained in the present study, it is possible to confirm the reparative potential of ozone therapy in a dose-dependent response, as well as its antimicrobial potential. However, studies are needed to elucidate the molecular mechanisms by which it exerts such biological effects.Item Ozonioterapia como um possível tratamento para dermatofitoses: estudo in vitro(Universidade Brasil, 2023) Espindola, Pâmela Petrângelo; Garcia, Lívia AssisItem Efeito antibacteriano do óleo de girassol ozonizado em diferentes concentrações(Universidade Brasil, 2021) Ficher, Ester; Nuñez, Silvia CristinaThe world is currently facing a crisis regarding microorganisms and microbial resistance. The evaluation of alternative antimicrobial therapies has been a constant search in recent decades. Ozonized oil can be an alternative within this field. The present study aims to analyze the antimicrobial efficacy of commercially available ozonated sunflower oil in different concentrations. A gram-positive bacteria Staphylococcus aureus and the gram-negative bacteria Escherichia coli were used. The microorganisms were grown in BHI broth (Brain and Heart Infusion) for 24h. Plates containing BHI agar were prepared for the inhibition halo test with a 3 mm diameter central well and contaminated with 50 µL of bacterial suspension. The wells made in the plates were filled with ozonated sunflower oil in concentrations ranging from 80 mmol-Eq / kg to 1290 mmol-Eq /. The plates were taken to the incubator at 37°C for 24h and the inhibition zone was measured with a millimeter probe, two control groups one with non-ozonated sunflower oil and one with 2% chlorhexidine gluconate solution were also evaluated. The results demonstrated that the efficacy for E.coli increases linearly with increasing concentration, however, only the concentrations of 702 mmol-Eq / kg and 1290 mmol-Eq / kg were significantly different (p<0.004). For S.aureus, the results showed significant differences only in the concentration of 1290 mmol-Eq / kg, but with a similar effect between the intermediate and higher concentrations (p<0.0001). The chlorhexidine group demonstrated to have a more effective action for both microorganisms under the conditions of this study (p<0.001). According to the results ozonized sunflower oil has less antimicrobial effect than chlorhexidine, the result is dependent on the concentration and only concentrations above 702 mmol-Eq / kg showed some efficacy.