Efeitos da fotobiomodulação associada ou não ao Biopolímero de Fibrina Heterólogo no processo de reparo tecidual de lesões cutâneas de ratos diabéticos

Abstract

INTRODUCTION: Diabetes mellitus is a general heterogeneous term that classifies metabolic disorders caused by increased glucose levels (hyperglycemia). Among the therapeutic modalities researched in order to accelerate tissue repair, LED photobiomodulation therapy and heterologous fibrin biopolymer stand out. PURPOSE: To evaluate the effects of LED photobiomodulation therapy associated or not with heterologous fibrin biopolymer in the tissue repair process of skin lesions in diabetic rats. METHODOLOGY: Forty-eight adult female Wistar rats (Rattus norvegicus albinus), 60 days old, weighing 230±20 g, from the vivarium of the State University of Piauí, were used. The animals were randomly divided into 4 distinct groups (control group, heterologous fibrin biopolymer group, LED photobiomodulation therapy group, LED photobiomodulation therapy group associated with heterologous fibrin biopolymer), which were treated in two experimental periods (7 and 14 days). Diabetes was induced by the administration of an aqueous solution of 2% alloxan and the animals had their blood glucose assessed using a glucometer and clinical symptoms. The animals underwent two surgical procedures, in the dorsal and cervical regions, the first by means of a skin punch, an area of 2 cm²; and the second a linear lesion measuring 3 cm, in the cervical region. Healing analysis was performed by calculating the contraction of the wound area and evaluating the traction force. RESULTS: The treatments with LED photobiomodulation therapy and heterologous fibrin biopolymer, used alone or in combination, indicated the stimulation of the repair process. The heterologous fibrin biopolymer group showed similar results to the LED photobiomodulation therapy group in wound regression, but showed better healing resistance, suggesting that the LED group has both repair qualities. The LED photobiomodulation therapy group associated with heterologous fibrin biopolymer showed better results in resistance and regression when compared to the heterologous fibrin biopolymer and LED photobiomodulation therapy groups, statistically closer to the control group. CONCLUSION: Based on the findings, it was observed that treatments with LED, BFH or LED associated with BFH acted satisfactorily, favoring the improvement in the tissue repair process, suggesting that these treatments, isolated or associated, were effective in stimulating the healing process in diabetic rats.