Desenvolvimento de processo de desinfecção por ozônio e conservação de pele de Lithobates catesbeianus para enxerto cutâneo em modelo animal

Abstract

Skin replacement is an important step in the treatment of various etiologies. Currently, there are several types of grafts available on the market, each with its own specificities, being used after a clinical study on the patient's health status. The use of bullfrog skin (Lithobates Catesbeianus) has shown promise as a possible skin graft, due to its cellular composition, high concentration of collagen, water and nutrients, biocompatibility, biological interaction with the substrate with the release of tissue biomodulators. In this work, a protocol for the sterilization and preservation of the bullfrog (Lithobates Catesbeianus) skins was developed, and its efficacy as a skin graft in an animal model was evaluated. Sterilization with ozone and conservation in 4 different ozonized solutions (saline solution, tea tree oil, copaiba oil and chitosan) were tested. The protocol of ozone disinfection and conservation in ozonized copaiba oil kept the skin inert to any microorganism for a period of twelve months. The evaluation in animal model was performed by histological analysis and by optical coherence tomography. These analyzes show promising results for future clinical applications, the skin proved to be excellent for use as a xenograft, as there was good graft adhesion, presence of fibroblasts and formation of a newly formed dermis. Compared to the autograft (control), this xenograft showed a greater presence of inflammatory infiltrates, which indicates the beginning of granulation tissue growth.