Monografias, Dissertações e Teses
URI permanente desta comunidadehttps://repositorioacademico.universidadebrasil.edu.br/handle/123456789/1
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Item Variabilidade da frequência cardíaca e níveis de cortisol salivar entre policiais militares(Universidade Brasil, 2020) Araújo, Liliam Mendes de; Santos, Laurita dosThis study aims to validate the relationship between heart rate variability patterns and salivary cortisol levels in military police officers, based on measurements induced by the Trier social Stress Test (TSST). This is a cross-sectional experimental study, conducted between the months of February and November 2020, involving 30 male police officers in regular working activities for a year or more, aging over 18. The study excluded individuals with high blood pressure, diabetes, those carrying a pacemaker or a transplanted organ and teachers. The subjects were submitted to the TSST and their HRV and saliva (in order to measure cortisol levels) were taken at three moments: basal period and prior to the TSST, immediately after the test and 20 minutes after the second collection. For collecting the temporal series of RR intervals, we used the Polar®, v800 monitor. The project was approved by the Ethics and Research Committee of Universidade Brasil, CAAE: 85502418.0.0000.5494. The results showed that the TSST induced a physiological response which can be visualized in the cortisol concentrations (0.272 ± 0.221 e mean 0.220 µg/dL), p = 0.0001. With HRV analyzed by using linear time domain, frequency domain and non-linear methods, it was possible to identify that some variables show statistic difference (p< 0.05) among the distinct moments of the experimental protocol. Among the main contributions of this study, it was possible to point out the importance of cortisol, showing that it is related to HRV linear and non-linear measurements, as well as the strong positive linear correlation between the HRV variables. Thus, HRV used as an information tag of the physiological impacts caused by the experimental stress causes, in addition to being adopted as a complementary tool for police officers’ clinical evaluation, can also be an important biomarker to promote health. Promoting military police officers’ health is a big challenge, once strategies aimed at fighting stress related to police activities must be implemented so as to improve the quality of life and health of these professionals.Item Estudo dos efeitos da hipóxia e anóxia durante o crescimento micelial de Metarhizium Robertsii na tolerância a estresses de conídios e regulação de genes(Universidade Brasil, 2022) Martins e Silva, Albery; Rangel, Drauzio Eduardo NarettoLittle is known about the impact of hypoxia and anoxia during mycelial growth on tolerance to different stress conditions of fungal conidia development. Metarhizium robertsii conidia were produced on potato dextrose agar (PDA) medium under normoxic (control = normal oxygen concentrations), continuous hypoxia and transient anoxia, as well as minimal medium. The tolerance of conidia produced under these different conditions was evaluated in relation to moist heat (thermal stress), menadione (oxidative stress), potassium chloride (osmotic stress), UV radiation and 4-nitroquinoline-1-oxide (genotoxic stress 4-NQO ). Growth under hypoxic conditions induced greater tolerance of M. robertsii conidia to menadione, KCl and UV radiation. The transient anoxic condition induced greater tolerance of conidia to KCl and UV radiation. Nutritional stress (ie, minimal medium) induced greater tolerance of conidia to heat, menadione, KCl and UV radiation. The hsp30 and hsp101 gene encoding a heat shock protein was overexpressed in molecular analysis under anoxic condition. In conclusion, growth under hypoxia and anoxia produced conidia with greater stress tolerance than conidia produced under normoxic conditions. The nutritional stress generated by the minimal medium, however, induced a much higher stress tolerance. This condition also caused the highest level of gene expression in the hsp30 and hsp101 genes. Allowing concluding that there was a greater adaptation to the stress of the conidia produced under nutritional stress, hypoxia and anoxia.